Phytochemical Analysis and Cytotoxic Activities of Hantap Leaves (Sterculia coccinea Jack) Extract

https://doi.org/10.22146/ijc.79362

Yuliet Yuliet(1*), Agustinus Widodo(2), Khildah Khaerati(3), Joni Tandi(4)

(1) Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Tadulako University, Jl. Sukarno-Hatta Km 9, Palu 94148, Indonesia
(2) Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Tadulako University, Jl. Sukarno-Hatta Km 9, Palu 94148, Indonesia
(3) Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Tadulako University, Jl. Sukarno-Hatta Km 9, Palu 94148, Indonesia
(4) Department of Pharmacy, College of Pharmacy and Natural Sciences Pelita Mas, Jl. Wolter Monginsidi No. 106 A, Palu 94111, Indonesia
(*) Corresponding Author

Abstract


Hantap (Sterculia coccinea Jack) has been used traditionally for various health issues, including cancer treatment. The therapeutic effects of natural ingredients are often attributed to their chemical constituents. This study aimed to analyze the phytochemical contents and cytotoxic activities of S. coccinea leaves on HeLa and MCF-7 cancer cell lines. The quantitative phytochemical analysis was carried out following standard laboratory procedures. Phytochemical compounds were identified using LC-MS/MS QTOF. The MTT assay PrestoBlue™ Cell Viability Reagent test method was used to test cytotoxic activity in the cell culture. Extraction was carried out by the maceration method using 96% ethanol as solvent. The quantitative analysis revealed that tannins were the major phytochemical constituent in the highest percentage of 72.16%, followed by alkaloids, flavonoids, and steroids, with values of 30.80, 28.66, and 2.85%, respectively. Saponins were present in the lowest percentage of 1.15%. The ethanolic extract exhibited moderate cytotoxicity on HeLa and MCF-7 cells with IC50 values of 591.00 and 578.10 µg/mL, respectively. Identification using LC-MS/MS showed the suspected compounds 5,7-dihydroxy-3-(4'-hydroxybenzyl)chromone as homoisoflavanones and kaempferide-3-O-α-L-rhamnosyl-7-O-α-L-rhamnoside from flavonol triglycosides. These results may contribute to the study on the use of leaves extract of S. coccinea for developing a chemoprevention agent.


Keywords


cytotoxicity; hantap leaves; HeLa; MTT assay; MCF-7

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References

[1] Kristina, S.A., Endarti, D., and Aditama, H., 2022, Prediction of productivity costs related to cervical cancer mortality in Indonesia 2018, Malays. J. Med. Sci., 29 (1), 138–144.

[2] The Global Cancer Observatory, 2020, Cancer Incident in Indonesia, Vol. 858, International Agency for Research on Cancer, Lyon, France.

[3] Islam, S.M.S., Purnat, T.D., Phuong, N.T.A., Mwingira, U., Schacht, K., and Fröschl, G., 2014, Non Communicable Diseases (NCDs) in developing countries: A symposium report, Global. Health, 10 (1), 81.

[4] Ministry of Health of Republic Indonesia, 2018, Laporan Nasional Riskesdas 2018, Ministry of Health of Republic Indonesia, Jakarta.

[5] Sung, H., Ferlay, J., Siegel, R.L., Laversanne, M., Soerjomataram, I., Jemal, A., and Bray, F., 2021, Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries, Ca-Cancer J. Clin., 71 (3), 209–249.

[6] American Cancer Society, 2018, Global Cancer-Facts & Figures, 4th Ed., American Cancer Society, Atlanta.

[7] Kurtin, S., 2012, Myeloid toxicity of cancer treatment, J. Adv. Pract. Oncol., 3 (4), 209–224.

[8] Lameire, N., 2014, Nephrotoxicity of recent anti-cancer agents, Clin. Kidney J., 7 (1), 11–22.

[9] Chen, Z., and Ai, D., 2016, Cardiotoxicity associated with targeted cancer therapies, Mol. Clin. Oncol., 4 (5), 675–681.

[10] Greenwell, M., and Rahman, P.K.S.M., 2015, Medicinal plants: Their use in anticancer treatment, Int. J. Pharm. Sci. Res., 6 (10), 4103–4112.

[11] Meiyanto, E., and Larasati, Y.A., 2019, The chemopreventive activity of Indonesia medicinal plants targeting on hallmarks of cancer, Adv. Pharm. Bull., 9 (2), 219–230.

[12] George, B.P, Chandran, R., and Abrahamse, H., 2021, Role of phytochemicals in cancer chemoprevention: Insights, Antioxidants, 10 (9), 1455.

[13] Radji, M., Aldrat, H., Harahap, Y., and Irawan, C., 2010, Penggunaan obat herbal pada pasien kanker serviks, JIFI, 8 (1), 33–39.

[14] Cragg, G.M., and Pezzuto, J.M., 2016, Natural products as a vital source for the discovery of cancer chemotherapeutic and chemopreventive agents, Med. Princ. Pract., 25 (Suppl. 2), 41–59.

[15] Dave, A., Parande, F., Park, E.J., and Pezzuto, J.M., 2020, Phytochemicals and cancer chemoprevention, J. Cancer Metastasis Treat., 6, 46.

[16] Effendi, N., 2012, Standarisasi simplisia daun hantap (Sterculia coccinea Jack) asal kabupaten Donggala propinsi Sulawesi Tengah sebagai bahan baku sediaan fitofarmaka, J. Sainsmat, 1 (1), 23–32.

[17] Rabbi, F., Zada, A., Adhikari, A., Nisar, A., and Khalil, S.K., 2021, Chemical constituent with cytotoxicity from Sterculia diversifolia, J. Asian Nat. Prod. Res., 23 (9), 899–905.

[18] Pham, N.K.T., Nguyen, T.D., Doan, T.D.C., Ha, T.D., Tran, N.M.A., Tran, T.D., and Nguyen, T.P., 2021, Stercufoetin A, new oleanane-type triterpenoid from the leaves of Sterculia foetida L., Nat. Prod. Res., 35 (7), 1226–1231.

[19] Liu, K, Abdullah, A.A., Huang, M., Nishioka, T., Altaf-Ul-Amin, M., and Kanaya, S., 2017, Novel approach to classify plants based on metabolite-content similarity, BioMed Res. Int., 2017, 5296729.

[20] Pourahmad, J., Salimi, A., and Seydi, E., 2016, "Role of oxygen free radicals in cancer development and treatment" in Free Radicals and Diseases, Eds. Ahmad, R., IntechOpen, Rijeka, Croatia.

[21] Lichota, A., and Gwozdzinski, K., 2018, Anticancer activity of natural compounds from plant and marine environment, Int. J. Mol. Sci., 19 (11), 3533.

[22] Lianah, L., Nur Khasanah, R.A., Pranatami, D.A., and Krisantini, K., 2021, Phytochemical screening and cytotoxic evaluation of Bauhinia scandens leaf extracts using HeLa and T47D cell lines, Biodiversitas, 22 (2), 913–919.

[23] Fakhrudin, N., Khairunnisa, S.Y., Azzahra, A., and Ajiningtyas, R.J., 2016, Study of radical scavenger activity, total phenol and flavonoid contents of Artocarpus altilis leaves extracts, Int. J. Pharm. Clin. Res., 8 (5), 352–356.

[24] Anbukkarasi, M., Dhamotharan, R., and Janarthanam, B., Studies on phytochemical screening, tannins content and antibacterial activity from leaf and callus extracts of Memecylon umbellatum, Asian J. Pharm. Clin. Res., 10 (5), 265–269.

[25] Hardianto, M.A., and Widiyanto, S., 2022, In vitro anthelmintic activity of Limonia acidissima, L. leaves aqueous extract on Haemonchus contortus (Rudolphi, 1803), Proceedings of the 7th International Conference on Biological Science (ICBS 2021), Atlantis Press, 73–77.

[26] Sutar, N.G., and Patil, P.P., 2020, A HPTLC densitometric method for the determination of β-sitosterol in Perguleria daemia leaf and stem extract, Int. J. Pharm. Sci. Res.,11 (4),1698–1704.

[27] Aldossary, S.A., 2019, Review on pharmacology of cisplatin: Clinical use, toxicity and mechanism of resistance of cisplatin, Biomed Pharmacol. J., 12 (1), 7–15.

[28] Alnuqaydan, A.M., Almutary, A.G., Alshehri, O.Y., Henidi, H.A., Alajlan, A.M., Al Tamim, A., Rather, M.Y., and Rah, B., 2022, Evaluation of the cytotoxic activity of Tamarix articulata and its anticancer potential in prostate cancer cells, J. Appl. Pharm. Sci., 12 (2), 89–108.

[29] Cahyani, R., Susanto, Y., and Khumaidi, A., 2017, Aktivitas antioksidan dan sitotoksik ekstrak etanol daun hantap (Sterculia coccinea Jack.), Nat. Sci.: J. Sci. Technol., 6 (1), 11–21.

[30] Mohamad, M., Ali, M.W., Ripin, A., and Ahmad, A., 2013, Effect of extraction process parameters on the yield of bioactive compounds from the roots of Eurycoma longifolia, J. Teknol., 60 (1), 51–57.

[31] Bibi, T., Ahmad, M., Bakhsh Tareen, R., Mohammad Tareen, N., Jabeen, R., Rehman, S.U., Sultana, S., Zafar, M., and Yaseen, G., 2014, Ethnobotany of medicinal plants in district Mastung of Balochistan province-Pakistan, J. Ethnopharmacol., 157, 79–89.

[32] Prastiwi, R., Dewanti, E., Fadliani, I.N., Aqilla, N., Salsabila, S., and Ladeska, V., 2020, The nephroprotective and antioxidant activity of Sterculia rubiginosa Zoll. Ex Miq. leaves, Pharmacogn. J., 12 (4), 843–849.

[33] El-Sherei, M.M., Ragheb, A.Y., Kassem, M.E.S., Marzouk, M.M., Mosharrafa, S.A., and Saleh, N.A.M., 2016, Phytochemistry, biological activities and economical uses of the genus Sterculia and the related genera: A review, Asian Pac. J. Trop. Dis., 6 (6), 492–501.

[34] Gad, S.C., 2015, "Alternatives to In vivo Studies in Toxicology" in General, Applied and Systems Toxicology, Eds. Ballantyne, B., Marrs, T.C., Syversen, T., Casciano, D.A., and Sahu, S.C., Wiley, Chichester, 15–47.

[35] Nath, L.R., Gorantla, J.N., Joseph, S.M., Antony, J., Thankachan, S., Menon, D.B., Sankar, S., Lankalapalli, R.S., and Anto, R.J., 2015, Kaempferide, the most active among the four flavonoids isolated and characterized from Chromolaena odorata, induces apoptosis in cervical cancer cells while being pharmacologically safe, RSC Adv., 5 (122), 100912–100922.

[36] Abotaleb, M., Samuel, S.M., Varghese, E., Varghese, S., Kubatka, P., Liskova, A., and Büsselberg, D., 2019, Flavonoids in cancer and apoptosis, Cancers, 11 (1), 28.

[37] Gupta, D., and Guliani, E., 2022, Flavonoids: Molecular mechanism behind natural chemoprotective behavior-A mini review, Biointerface Res. Appl. Chem., 12 (5), 5983–5995.

[38] Baer-Dubowska, W., Szaefer, H., Majchrzak-Celińska, A., and Krajka-Kuźniak, V., 2020, Tannic acid: Specific form of tannins in cancer chemoprevention and therapy-old and new applications, Curr. Pharmacol. Rep., 6 (2), 28–37.

[39] Ballout, F., Habli, Z., Monzer, A., Rahal, O.N., Gali-Muhtasib, H., and Fatfat, M., 2019, "Anticancer Alkaloids: Molecular Mechanisms and Clinical Manifestations" in Bioactive Natural Products for the Management of Cancer: From Bench to Bedside, Eds. Sharma, A.K., Springer, Singapore, 1–35.

[40] Tilaoui, M., Ait, Mouse, H., and Zyad, A., 2021, Update and new insights on future cancer drug candidates from plant-based alkaloids, Front. Pharmacol., 12, 719694.

[41] Elekofehinti, O.O., Iwaloye, O., Olawale, F., and Ariyo, E.O., 2021, Saponins in cancer treatment: Current progress and future prospects, Pathophysiology, 28 (2), 250–272.

[42] Hou, Y., Shang, C., Meng, T., and Lou, W., 2021, Anticancer potential of cardiac glycosides and steroid-azole hybrids, Steroids, 171, 108852.



DOI: https://doi.org/10.22146/ijc.79362

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