Evaluation of Phytochemical Properties and Biological Activities of Leaf Extracts and Oil of Petroselinum sativum Collected from Algeria


Lotfi Benmekhbi(1*), Salima Mosbah(2), Hanane Laamraoui(3), Ikram Hamlaoui(4), Serhouda Bencheriet(5), Demirtas Ibrahim(6)

(1) Laboratory of Molecular Chemistry, University of Frères Mentouri Constantine 1, Route de Ain El-Bey, 25000 Constantine, Algeria; Department Process Engineering, Salah Boubnider Constantine 3 University, Route de Ain El-Bey, 25000 Constantine, Algeria
(2) Laboratory of Molecular Chemistry, University of Frères Mentouri Constantine 1, Route de Ain El-Bey, 25000 Constantine, Algeria
(3) Department of Chemistry, University Larbi Ben M’hidi Oum El Bouaghi, Route de Constantine, 04000 Oum El Bouaghi, Algeria
(4) Laboratory of Molecular Chemistry, University of Frères Mentouri Constantine 1, Route de Ain El-Bey, 25000 Constantine, Algeria
(5) Laboratory of Molecular Chemistry, University of Frères Mentouri Constantine 1, Route de Ain El-Bey, 25000 Constantine, Algeria
(6) Department of Chemistry, Faculty of Science, Cankiri Karatekin University, 18100 Cankiri, Turkey
(*) Corresponding Author


Indigenous medical professionals prescribe plant-based therapeutics for the handling of a range of maladies and use typical medical care at the preventive level. Oxidative stress, obesity, and multidrug resistance to pathogenic microorganisms are major challenges in the healthcare systems and pharmaceutical industries. This study aimed to screen phytoconstituents, estimate total phenol and flavonoid contents, and evaluate the antioxidant, and antimicrobial activities of Petroselinum sativum extracts and essential oil growing in Algeria. The antioxidant capacity of n-butanol, dichloromethane extracts and essential oil was determined by using DPPH radical scavenging method. All extracts were also evaluated against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacterial species using the disk diffusion method. The leaves are pharmacologically active and related to the detected phytochemical components. In the proximate analysis, plant nutrients that are important for diverse pharmacological activities are found in the leaves. The ethyl acetate extract of P. sativum showed the highest phenol content while the n-butanol and ethyl acetate extracts gave high flavonoid contents at 144.44 ± 0.07 and 100.97 ± 0.04 mg/g, respectively. The antioxidant results demonstrate powerful free radical scavenging activity for three extracts. The activity reduced in the following order as concentration increased n-BuOH > AE > DCM.


Petroselinum sativum; phytochemical screening; antimicrobial properties; antifungal activity

Full Text:

Full Text PDF


[1] Altemimi, A., Lakhssassi, N., Baharlouei, A., Watson, D.G., and Lightfoot, D.A., 2017, Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts, Plants, 6 (4), 42.

[2] Jamshidi-Kia, F., Lorigooini, Z., and Amini-Khoei, H., 2018, Medicinal plants: Past history and future perspective, J. Herbmed Pharmacol., 7 (1), 1–7.

[3] Lim, T.K., 2013, Edible Medicinal and Non-Medicinal Plants, Vol. 6. Fruits, Springer, Dordrecht.

[4] Agyare, C., Appiah, T., Boakye, Y.D., and Apenteng, J.A., 2017, “Chapter 25 - Petroselinum crispum: A Review” in Medicinal Spices and Vegetables from Africa, Eds. Kuete, V., Academic Press, Cambridge, US, 527–547.

[5] Awe, E.O., and Banjoko, S.O., 2013, Biochemical and haematological assessment of toxic effects of the leaf ethanol extract of Petroselinum crispum (Mill) Nyman Ex A.W. Hill (Parsley) in rats, BMC Complementary Altern. Med., 13 (1), 75.

[6] Charles, D., 2012, “24 – Parsley” in Handbook of Herbs and Spices, 2nd Ed., Eds. Peter, K.V., Elsevier, Woodhead Publishing, Sawston, Cambridge, 430–451.

[7] Farzaei, M.H., Abbasabadi, Z., Ardekani, M.R.S., Rahimi, R., and Farzaei, F., 2013, Parsley: A review of ethnopharmacology, phytochemistry and biological activities, J. Tradit. Chin. Med., 33 (6), 815–826.

[8] Chaves, D.S.A. Frattani, F.S., Assafim, M., de Almeida, A.P., Zingali, R.B., and Costa, S.S., 2011, Phenolic chemical composition of Petroselinum crispum extract and its effect on haemostasis, Nat. Prod. Commun., 6 (7), 961–964.

[9] Nurcholis, W., Khumaida, N., Syukurs, M., Bintang, M., and Ardyani, I.D.A.A.C., 2015, Phytochemical screening, antioxidant and cytotoxic activities in extracts of different rhizome parts from Curcuma aeruginosa Roxb, Int. J. Res. Ayurveda Pharm., 6 (5), 634–637.

[10] Liu, P.Y., Liu, D., Li, W.H., Zhao, T., Sauriol, F., Gu, Y.C., Shi, Q.W., and Zhang, M.L., 2015, Chemical constituents of plants from the genus Eupatorium (1904–2014), Chem. Biodivers., 12 (10), 1481–515.

[11] Harborne, J.B., 1973, Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis, 3rd Ed., Chapman and Hall, New York.

[12] Savithramma, N., Rao, M.L., and Ankanna, S., 2012, Preliminary phytochemical screening of some important medicinal plants, Int. J. Ayurveda Res., 2 (1), 139–145.

[13] Trifa, W., Akkal, S., Lefahal, M., Benmekhebi, L., and Khennouf, S., 2020, Preliminary screening of Nicotiana glauca extracts for determination of antioxidant activity by different method, Curr. Issues Pharm. Med. Sci., 33 (1), 32–37.

[14] Fitriana, W.D., Ersam, T., Shimizu, K., and Fatmawati, S., 2016, Antioxidant activity of Moringa oleifera extracts, Indones. J. Chem., 16 (3), 297–301.

[15] Ghout, A., Zellagui, A., Gherraf, N., Demirtas, I., Sahin, Y.A., Boukhenaf, M., Lahouel, M., Nieto, G., and Akkal, S., 2018, Antiproliferative and antioxidant activities of two extracts of the plant species Euphorbia dendroides L., Medicines, 5 (2), 36.

[16] Charoensin, S., 2014, Antioxidant and anticancer activities of Moringa oleifera leaves, J. Med. Plant Res., 8 (7), 318–325.

[17] Zeghad, F., Djilani, S.E., Djilani, A., and Dicko, A., 2016, Antimicrobial and antioxidant activities of three Euphorbia species, Turk. J. Pharm. Sci., 13 (1), 47–56.

[18] Getahun, T, Sharma, V., and Gupta, N., 2020, Chemical composition and biological activity of essential oils from Aloe debrana roots, J. Essent. Oil-Bear. Plants, 23 (3), 493–502.

[19] Puttarak, P., Charoonratana, T., and Panichayupakaranant, P., 2010, Antimicrobial activity and stability of rhinacanthins-rich Rhinacanthus nasutus extract, Phytomedicine, 17 (5), 323–327.

[20] Derakhshan, Z., Ferrante, M., Tadi, M., Ansari, F., Heydari, A., Hosseini, M.S., Conti, G.O., and Sadrabad, E.K., 2018, Antioxidant activity and total phenolic content of ethanolic extract of pomegranate peels, juice, and seeds, Food Chem. Toxicol., 114, 108–111.

[21] Talla, E., Tamfu, A.N., Gade, I.S., Yanda, L., Mbafor, J.T., Laurent, S., Elst, L.V., Popova, M., and Bankova, V., 2016, New mono-ether of glycerol and triterpenes with DPPH radical scavenging activity from Cameroonian propolis, Nat. Prod. Res., 31 (12), 1379–1389.

[22] Aktumsek, A., Zengin, G., Guler, G.O., Cakmak, Y.S., and Duran, A., 2013, Antioxidant potentials and anticholinesterase activities of methanolic and aqueous extracts of three endemic Centaurea L. species, Food Chem. Toxicol., 55, 290–296.

[23] Habibou, H.H., Idrissa, M., Khalid, I., Ouedraogo, B., and Rabani, A., 2019, Antioxidant activity of methanolic extracts from various organs of Detarium microcarpum Guill. & Perr, Eur. Sci. J., 15 (12), 1857–7881.

[24] Sliguoua, M., Mahdi, I., Amrati, F.E.Z., Di Cristo, F., Amaghnouje, A., Grafov, A., Boucetta, N., Bari, A., and Bousta, D., 2021, Assessment of in vivo estrogenic and anti-inflammatory activities of the hydro-ethanolic extract and polyphenolic fraction of parsley (Petroselinum sativum Hoffm.), J. Ethnopharmacol., 265, 113290.

DOI: https://doi.org/10.22146/ijc.74370

Article Metrics

Abstract views : 1835 | views : 1256

Copyright (c) 2022 Indonesian Journal of Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.


Indonesian Journal of Chemistry (ISSN 1411-9420 /e-ISSN 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

Analytics View The Statistics of Indones. J. Chem.