Enhancing the Amino Acid and Reducing the Metal Ions Contents in the Hydrolysate Resulting from Hydrothermal Carbonization of Chicken Feather Waste by Chemical Phosphorylation


Agus Kuncaka(1*), Wahyu Tri Supardi(2), Winarto Haryadi(3), Adhitasari Suratman(4), Priatmoko Priatmoko(5)

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
(2) Department of Computer Science and Electronics, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
(3) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
(4) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
(5) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
(*) Corresponding Author


Chemical phosphorylation of hydrolysate resulting from hydrothermal carbonization of chicken feather waste was performed to enhance the amino acids and reduce the metal ions content. The aim of this research is to improve the functional properties of chicken feathers hydrolysate without impairing the nutritional availability thereof with the cheapest chemical method by phosphorylation. Phosphorylated hydrolysate can function as animal feed and fertilizer. The hydrolysate of chicken feathers was obtained by hydrothermal carbonization in an alkaline condition using a CaO and KOH catalyst, by the ratio of water:dry matter of chicken feathers is 5:1, at 9–10 atm pressure, and in a temperature of 190–200 °C during 3 h. Phosphorylation has been carried out by reacting the hydrolysate with H3PO4 85% in pH of 5, 6, 7 and using the original hydrolysate as control. The sample that has been prepared was characterized and semi-quantitative analyzed by HPLC and AAS. The phosphorylation results showed that the total maximum protein of soluble protein, their minimum metal ions, and anion in soluble protein was obtained at pH 7, while the higher the pH, the lower the liquid protein that was obtained.


hydrothermal carbonization; phosphorylation; liquid protein

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[1] Ayilara, M.S., Olanrewaju, O.S., Babalola, O.O., and Odeyemi, O., 2020, Waste management through composting: Challenges and potentials, Sustainability, 12 (11), 4456.

[2] da Silva, R.R., 2018, Keratinases as an alternative method designed to solve keratin disposal on the environment: Its relevance on agricultural and environmental chemistry, J. Agric. Food Chem., 66 (28), 7219–7221.

[3] Said, M.I., 2020, Potential development of poultry feather waste resources as raw material in industry: A review, IOP Conf. Ser.: Earth Environ. Sci., 492, 012089.

[4] Azizah, A., Nurhayati, N., Hendalia, E., and Maghfiro, S.R., 2021, Solubility of dry matter and protein of hydrolyzed feather meal fermented by Lactobacillus casei Shirota, J. Hunan Univ. Nat. Sci., 48 (11), 174–179.

[5] Lange, L., Huang, Y., and Busk, P.K., 2016, Microbial decomposition of keratin in nature—a new hypothesis of industrial relevance, Appl. Microbiol. Biotechnol., 100 (5), 2083–2096.

[6] Endo, R., Kamei, K., Iida, I., and Kawahara, Y., 2008, Dimensional stability of waterlogged wood treated with hydrolyzed feather keratin, J. Archaeol. Sci., 35 (5), 1240–1246.

[7] Poole, A.J., and Church, J.S., 2015, The effects of physical and chemical treatments on Na2S produced feather keratin films, Int. J. Biol. Macromol., 73, 99–108.

[8] Arifin, T., 2008, Pemanfaatan Limbah Bulu Ayam Potong Metode Pengukusan Untuk Bahan Ransum Ayam Potong, Thesis, Pengelolaan Sumber Daya Alam dan Lingkungan, Universitas Sumatera Utara, Medan, Indonesia.

[9] Joardar, J.C., and Rahman, M.M., 2018, Poultry feather waste management and effects on plant growth, Int. J. Recyl. Org. Waste Agric., 7 (3), 183–188.

[10] Jeong, J.H., Lee, O.M., Jeon, Y.D., Kim, J.D., Lee, N.R., Lee, C.Y., and Son, H.J., 2010, Production of keratinolytic enzyme by a newly isolated feather-degrading Stenotrophomonas maltophilia that produces plant growth-promoting activity, Process Biochem., 45 (10), 1738–1745.

[11] Joshi, S.G., Tejashwini, M.M., Revati, N., Sridevi, R., and Roma, D., 2007, Isolation, identification, and characterization of a feather degrading bacterium, Int. J. Poult. Sci., 6 (9), 689–693.

[12] Parry, D.A.D., and North, A.C.T., 1998, Hard α-keratin intermediate filament chains: Substructure of the N and C terminal domains and the predicted structure and function of the C-terminal domains of type I and type II chains, J. Struct. Biol., 122 (1), 67–75.

[13] Zhao, W., Yang, R., Zhang, Y., and Wu, L., 2012, Sustainable and practical utilization of feather keratin by an innovative physicochemical pretreatment: High density steam flash-explosion, Green Chem., 14 (12), 3352–3360.

[14] Vidmar, B., and Vodovnik, M., 2018, Microbial keratinases: Enzymes with promising biotechnological applications, Food Technol. Biotechnol., 56 (3), 312–328.

[15] Mazotto, A.M., de-Melo, A.C.N., Macrae, A., Rosado, A.S., Peixoto, R., Cedrola, S., Couri, S., Zingali, R.B., Villa, A.L.V., Rabinovitch, L., Chaves, J.Q., and Vermelho, A.B., 2011, Biodegradation of feather waste by extracellular keratinases and gelatinases from Bacillus spp., World J. Microbiol. Biotechnol., 27 (6), 1355–1365.

[16] Li, Q., 2022, Perspectives on converting keratin-containing wastes into biofertilizers for sustainable agriculture, Front. Microbiol., 13, 918262.

[17] Al Mousa, A.A., Moubayed, N.M.S., Al Jaloud, A.M., Al Khattaf, F.S., and Dahmasha, N.D., 2021, Chicken feathers waste management by microbial as a sustainable and tool environmental friendly, J. Environ. Prot., 12 (9), 639–653.

[18] Ramnani, P., Singh, R., and Gupta, R., 2005, Keratinolytic potential of Bacillus licheniformis RG1: Structural and biochemical mechanism of feather degradation, Can. J. Microbiol., 51 (3), 191–196.

[19] Adiati, U., Puastuti, W., Mathius, I., 2004, Peluang pemanfaatan tepung bulu ayam sebagai bahan pakan ternak ruminansia, Wartazoa, 14 (1), 39–44.

[20] Aragón-Briceño, C.I., Pozarlik, A.K., Bramer, E.A., Niedzwiecki, L., Pawlak-Kruczek, H., and Brem, G., 2021, Hydrothermal carbonization of wet biomass from nitrogen and phosphorus approach: A review, Renewable Energy, 171, 401–415.

[21] Ischia, G., and Fiori, L., 2021, Hydrothermal carbonization of organic waste and biomass: A review on process, reactor, and plant modeling, Waste Biomass Valorization, 12 (6), 2797–2824.

[22] Chaiammart, N., Eiad-ua, A., and Panomsuwan, G., 2020, Hydrothermal carbonization synthesis and KOH activation of porous carbons from waste marigold flowers, IOP Conf. Ser.: Mater. Sci. Eng., 773, 012048.

[23] Sengottian, M., Venkatachalam, C.D., and Ravichandran, S.R., 2022, Optimization of alkali catalyzed hydrothermal carbonization of Prosopis juliflora woody biomass to biochar for copper and zinc adsorption and its application in supercapacitor, Int. J. Electrochem. Sci., 17, 220938.

[24] Tronina, P., and Bubel, F., 2008, Production of organic fertilizer from poultry feather wastes excluding the composting process, Pol. J. Chem. Technol., 10 (2), 33–36.

[25] Onifade, A.A., Al-Sane, N.A., Al-Musallam, A.A., and Al-Zarban, S., 1998, A review: Potentials for biotechnological applications of keratin-degrading microorganisms and their enzymes for nutritional improvement of feathers and other keratins as livestock feed resources, Bioresour. Technol., 66 (1), 1–11.

[26] Yang, F., and Antonietti, M., 2020, Artificial humic acids: Sustainable materials against climate change, Adv. Sci., 7 (5), 1902992.

[27] Alhnidi, M.J., Körner, P., Wüst, D., Pfersich, J., and Kruse, A., 2020, Nitrogen-containing hydrochar: The influence of nitrogen-containing compounds on the hydrochar formation, ChemistryOpen, 9 (8), 864–873.

[28] Kruse, A., and Dahmen, N., 2015, Water – A magic solvent for biomass conversion, J. Supercrit. Fluids, 96, 36–45.

[29] Neal, J.F., Zhao, W., Grooms, A.J., Smeltzer, M.A., Shook, B.M., Flood, A.H., and Allen, H.C., 2019, Interfacial supramolecular structures of amphiphilic receptors drive aqueous phosphate recognition, J. Am. Chem. Soc., 141 (19), 7876–7886.

[30] Hargrove, A.E., Nieto, S., Zhang, T., Sessler, J.L., and Anslyn, E.V., 2011, Artificial receptors for the recognition of phosphorylated molecules, Chem. Rev., 111 (11), 6603−6782.

[31] Jastrzab, R., Nowak, M., Zabiszak, M., Odani, A., and Kaczmarek, M.T., 2021, Significance and properties of the complex formation of phosphate and polyphosphate groups in particles present in living cells, Coord. Chem. Rev., 435, 213810.

[32] Gowri, S., Uma Devi, T., Alen, S., Sajan, D., Surendra Dilip, C., and Vinitha, G., 2018, Synthesis, crystal structure, optical and third order nonlinear optical properties of phosphoric acid pyridine-1-ium-2-carboxylate, J. Mater. Sci.: Mater. Electron., 29 (23), 19710–19723.

DOI: https://doi.org/10.22146/ijc.73725

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