Effect of Ascorbic Acid Concentration on the Stability of Tartrate-Capped Silver Nanoparticles


Indah Miftakhul Janah(1), Roto Roto(2), Dwi Siswanta(3*)

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
(3) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
(*) Corresponding Author


In this work, tartrate-capped silver nanoparticles (AgNPs) by reducing Ag+ ions into Ag0 using L-ascorbic acid and capping disodium tartrate have been prepared. The reaction was carried out at room temperature in an alkaline medium of pH 11 to obtain a rapid and one-step green synthesis method. The effect of L-ascorbic acid concentration on the synthesis preparation was studied to investigate their impact on the particle size, morphology, and stability of the AgNPs. The obtained tartrate capped AgNPs have SPR absorbance in 390–410 nm. They have a spherical shape, as confirmed by TEM. Increasing L-ascorbic acid concentrations from 25 mM to 100 and 200 mM leads to the 27, 17, and 11 nm particle size distributions. They give the zeta potential of –33.5, –20.8, and –21.3, respectively. After a week, the decreasing absorbance peaks were 0.151, 0.0105, and 0.336 a.u. The optimum L-ascorbic acid concentration was obtained at 100 mM, indicated by the smallest FWHM point. Thus, we may conclude that lower or higher levels of reducing agents resulted in low stability. Therefore, controlling L-ascorbic acid concentration is an important parameter. A sufficient concentration and an appropriate capping agent can produce good nanoparticle stability essential for further application.


silver nanoparticles; L-ascorbic acid; disodium tartrate; green synthesis; stability

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[1] Xiong, J., Wang, Y., Xue, Q., and Wu, X., 2011, Synthesis of highly stable dispersions of nanosized copper particles using L-ascorbic acid, Green Chem., 13 (4), 900–904.

[2] Martinez-Andrade, J.M., Avalos-Borja, M., Vilchis-Nestor, A.R., Sanchez-Vargas, L.O., and Castro-Longoria, E., 2018, Dual function of EDTA with silver nanoparticles for root canal treatment–A novel modification, PLoS One, 13 (1), 1–19.

[3] Guimarães, M.L., da Silva, F.A.G., da Costa, M.M., and de Oliveira, H.P., 2020, Green synthesis of silver nanoparticles using Ziziphus joazeiro leaf extract for production of antibacterial agents, Appl. Nanosci., 10 (4), 1073–1081.

[4] Xia, Y., Zhu, C., Bian, J., Li, Y., Liu, X., and Liu, Y., 2019, Highly sensitive and selective colorimetric detection of creatinine based on synergistic effect of PEG/Hg2+–AuNPs, Nanomaterials, 9 (10), 1424.

[5] Li, J., Hou, C., Huo, D., Shen, C., Luo, X., Fa, H., Yang, M., and Zhou, J., 2017, Detection of trace nickel ions with a colorimetric sensor based on indicator displacement mechanism, Sens. Actuators, B, 241, 1294–1302.

[6] Yakoh, A., Rattanarat, P., Siangproh, W., and Chailapakul, O., 2018, Simple and selective paper-based colorimetric sensor for determination of chloride ion in environmental samples using label-free silver nanoprisms, Talanta, 178, 134–140.

[7] Ma, Y., Pang, Y., Liu, F., Xu, H., and Shen, X., 2016, Microwave-assisted ultrafast synthesis of silver nanoparticles for detection of Hg2+, Spectrochim. Acta, Part A, 153, 206–211.

[8] Caro, C., Castillo, P.M., Klippstein, R., Pozo, D., and Zaderenko, A.P., 2010, "Silver Nanoparticles: Sensing and Imaging Applications" in Silver Nanoparticles, Eds. Perez, D.P., IntechOpen, Rijeka, Croatia, 201–225.

[9] Phan, H.T., and Haes, A.J., 2019, What does nanoparticle stability mean?, J. Phys. Chem. C, 123 (27), 16495–16507.

[10] Riaz Ahmed, K.B., Nagy, A.M., Brown, R.P., Zhang, Q., Malghan, S.G., and Goering, P.L., 2017, Silver nanoparticles: Significance of physicochemical properties and assay interference on the interpretation of in vitro cytotoxicity studies, Toxicol. in Vitro, 38, 179–192.

[11] Elahi, N., Kamali, M., and Baghersad, M.H., 2018, Recent biomedical applications of gold nanoparticles: A review, Talanta, 184, 537–556.

[12] Beyene, H.D., Werkneh, A.A., Bezabh, H.K., and Ambaye, T.G., 2017, Synthesis paradigm and applications of silver nanoparticles (AgNPs), a review, Sustainable Mater. Technol., 13, 18–23.

[13] Shnoudeh, A.J., Hamad, I., Abdo, R.W., Qadumii, L., Jaber, A.Y., Salim, H., and Alkelany, S.Z., 2019, "Synthesis, Characterization, and Applications of Metal Nanoparticles" in Biomaterials and Bionanotechnology, Eds. Tekade, R.K., Academic Press, Cambridge, US, 527–612.

[14] Jang, K.I., and Lee, H.G., 2008, Stability of stability of chitosan nanoparticles for L-ascorbic acid during heat treatment in aqueous solution, J. Agric. Food Chem., 56 (6), 1936–1941.

[15] Qin, Y., Ji, X., Jing, J., Liu, H., Wu, H., and Yang, W., 2010, Size control over spherical silver nanoparticles by ascorbic acid reduction, Colloids Surf., A, 372 (1-3), 172–176.

[16] Roto, R., Rasydta, H.P., Suratman, A., and Aprilita, N.H., 2018, Effect of reducing agents on physical and chemical properties of silver nanoparticles, Indones. J. Chem., 18 (4), 614–620.

[17] Annur, S., Santosa, S.J., and Aprilita, N.H., 2018, PH dependence of size control in gold nanoparticles synthesized at room temperature, Orient. J. Chem., 34 (5), 2305–2312.

[18] Chakraborty, I., Rakshit, R., and Mandal, K., 2017, Synthesis and functionalization of MnFe2O4 nano−hollow spheres for novel optical and catalytic properties, Surf. Interfaces, 7, 106–112.

[19] Abdel-Kader, M.M., El-Kabbany, F., and Taha, S., 1990, Physical properties and phase transitions in sodium tartrate dihydrate, J. Mater. Sci.: Mater. Electron., 1 (4), 201–203.

[20] Jain, S., Jain, A., Kachhawah, P., and Devra, V., 2015, Synthesis and size control of copper nanoparticles and their catalytic application, Trans. Nonferrous Met. Soc. China, 25 (12), 3995–4000.

[21] Wang, X., Yang, D.P., Huang, P., Li, M., Li, C., Chen, D., and Cui, D., 2012, Hierarchically assembled Au microspheres and sea urchin-like architectures: Formation mechanism and SERS study, Nanoscale, 4 (24), 7766–7772.

[22] González-Mendoza, A.L., and Cabrera-Lara, L.I., 2015, Reaction parameters for controlled sonosynthesis of gold nanoparticles, J. Mex. Chem. Soc., 59 (2), 119–129.

[23] Shitu, I.G., Talib, Z.A., Chi, J.L.Y., Kechick, M.M.A., and Baqiah, H., 2020, Influence of tartaric acid concentration on structural and optical properties of CuSe nanoparticles synthesized via microwave assisted method, Results Phys., 17, 103041.

[24] Wu, X., Xu, Y., Dong, Y., Jiang, X., and Zhu, N., 2013, Colorimetric determination of hexavalent chromium with ascorbic acid capped silver nanoparticles, Anal. Methods, 5 (2), 560–565.

[25] Xu, Y., Dong, Y., Jiang, X., and Zhu, N., 2013, Colorimetric detection of trivalent chromium in aqueous solution using tartrate-capped silver nanoparticles as probe, J. Nanosci. Nanotechnol., 13 (10), 6820–6825.

[26] Rycenga, M., Cobley, C.M., Zeng, J., Li, W., Moran, C.H., Zhang, Q., Qin, D., and Xia, Y., 2011, Controlling the synthesis and assembly of silver nanostructures for plasmonic applications, Chem. Rev., 111 (6), 3669–3712.

[27] Sharma, R., Dhillon, A., and Kumar, D., 2018, Mentha-stabilized silver nanoparticles for high-performance colorimetric detection of Al(III) in aqueous system, Sci. Rep., 8 (1), 5189.

[28] Kim, H., Seo, Y.S., Kim, K., Han, J.W., Park, Y., and Seonho, C., 2016, Concentration effect of reducing agents on green synthesis of gold nanoparticles: Size, morphology, and growth mechanism, Nanoscale Res. Lett., 11, 230.

[29] Du, H., Chen, R., Du, J., Fan, J., and Peng, X., 2016, Gold nanoparticle-based colorimetric recognition of creatinine with good selectivity and sensitivity, Ind. Eng. Chem. Res., 55 (48), 12334–12340.

[30] Zain, N.M., Stapley, A.G.F., and Shama, G., 2014, Green synthesis of silver and copper nanoparticles using ascorbic acid and chitosan for antimicrobial applications, Carbohydr. Polym., 112, 195–202.

DOI: https://doi.org/10.22146/ijc.73196

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