Identification of Sida rhombifolia from Its Related Plants Using Thin-Layer Chromatographic Analysis

Mohamad Rafi(1*), Sefni Reza Yolanda(2), Dewi Anggraini Septaningsih(3), Maria Bintang(4), Nanik Siti Aminah(5), Muhamad Insanu(6), Abdul Rohman(7)

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Jl. Tanjung Kampus IPB Dramaga, Bogor 16680, Indonesia; Tropical Biopharmaca Research Center - Institute of Research and Community Services, IPB University, Jl. Taman Kencana No. 3 Kampus IPB Taman Kencana, Bogor 16128, Indonesia
(2) Department of Biochemistry, Faculty of Mathematics and Natural Sciences, IPB University, Jl. Tanjung Kampus IPB Dramaga, Bogor 16680, Indonesia
(3) Tropical Biopharmaca Research Center - Institute of Research and Community Services, IPB University, Jl. Taman Kencana No. 3 Kampus IPB Taman Kencana, Bogor 16128, Indonesia; Advanced Research Laboratory - Institute of Research and Community Services, IPB University, Jl. Palem Raya Kampus IPB Dramaga, Bogor 16680, Indonesia
(4) Department of Biochemistry, Faculty of Mathematics and Natural Sciences, IPB University, Jl. Tanjung Kampus IPB Dramaga, Bogor 16680, Indonesia
(5) Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Jl. Mulyorejo Kampus C Unair, Surabaya 60286, Indonesia
(6) School of Pharmacy, Institut Teknologi Bandung, Jl. Ganesha No. 10, Bandung 40132, Indonesia
(7) Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
(*) Corresponding Author


Sida rhombifolia belongs to the Malvaceae family and is often used to treat gout in Indonesia. S. rhombifolia has many efficacies and contains many different chemical components. The abundance and variation of chemical content and chemical compounds in this medicinal plant are challenging factors in ensuring medicinal plants' safety and quality control. Thin-layer chromatography (TLC) fingerprint analysis derived from S. rhombifolia extract can also be used for the medicinal plant's quality control. This research aimed to develop the optimum condition for the chemical fingerprint analysis of S. rhombifolia using a TLC fingerprint analysis. A total of 11 bands were produced with optimum separation using silica gel 60 F254 TLC plate, a mixed mobile phase condition with chloroform, ethyl acetate, and methanol (6.5:2:1.5). This fingerprint analysis performed an excellent separation in the TLC plate at 366 nm with sulfuric acid as reagent derivatization. In general, the results of the analysis validation, including stability, specificity, precision, and robustness of TLC fingerprints, met the acceptance criteria. The TLC fingerprint of S. rhombifolia can be distinguished from 2 related plants with similar leaf shapes, Turnera ulmifolia L. and Hibiscus rosa-sinensis. The developed method was validated, so it could be used to control S. rhombifolia quality.


fingerprint analysis; identification; Sida rhombifolia; thin-layer chromatography

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