Antioxidant Flavonoid Glycoside from Leaves of Cacao Mistletoe (Scurrula ferruginea (Jack) Danser)

Mai Efdi(1*), Dara Pratama(2), Afrizal Itam(3), Tia Okselni(4)

(1) Department of Chemistry, Universitas Andalas, Kampus Limau Manis, Padang 25163, West Sumatra, Indonesia
(2) Department of Chemistry, Universitas Andalas, Kampus Limau Manis, Padang 25163, West Sumatra, Indonesia
(3) Department of Chemistry, Universitas Andalas, Kampus Limau Manis, Padang 25163, West Sumatra, Indonesia
(4) Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency, Jl. Raya Bogor Km 46, Cibinong, Bogor 16911, West Java, Indonesia
(*) Corresponding Author


Scurrula ferruginea (Jack) Danser is a folk medicine to treat several diseases. S. ferruginea is the mistletoe that lives by deriving nutrients from the host. Hence, the host has a significant effect on the biological activities and bioactive components of S. ferruginea. In this study, the leaves of S. ferruginea were macerated by methanol solvent to extract the chemical components and fractionated by hexane and ethyl acetate solvent, respectively, to separate organic compounds. The biological activity of crude extracts as the antioxidant was investigated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The result showed that the highest antioxidant activity was obtained from the ethyl acetate fraction with the IC50 of 10.88 ± 0.39 µg/mL. Furthermore, the trituration method used several solvents to isolate secondary metabolites from the initial crude methanol extract. This purification process produced a flavonoid compound of quercitrin. The antioxidant activity of quercitrin was also determined by the DPPH method. The result showed that this compound has stronger activity than the crude extracts with the IC50 of 5.95 ± 0.11 µg/mL. This study demonstrated that cacao mistletoe of S. ferruginea may have the potential activity to treat the diseases caused by the presence of free radicals.


Scurrula ferruginea leaves; antioxidant; quercitrin

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[1] Lim, Y.C., Rajabalaya, R., Lee, S.H.F., Tennakoon, K.U., Le, Q.V., Idris, A., Zulkipli, I.N., Keasberry, N., and David, S.R., 2016, Parasitic mistletoes of the Genera Scurrula and Viscum: From bench to bedside, Molecules, 21 (8), 1048.

[2] Moghadamtousi, S.Z., Kamarudin, M.N.A., Chan, C.K., Goh, B.H., and Kadir, H.A., 2014, Phytochemistry and biology of Loranthus parasiticus Merr, a commonly used herbal medicine, Am. J. Chin. Med., 42 (1), 23–35.

[3] Le, Q.V., Tennakoon, K.U., Metali, F., Lim, L.B.L., and Bolin, J.F., 2016, Ecophysiological responses of mistletoe Dendrophthoe curvata (Loranthaceae) to varying environmental parameters, J. Trop. For. Sci., 28 (1), 59–67.

[4] Le, Q.V., Tennakoon, K.U., Metali, F., Lim, L.B.L., and Bolin, J.F., 2016, Host specific variation in photosynthesis of an obligate xylem-tapping mistletoe Dendrophthoe curvata in a Bornean heath forest, Nord. J. Bot., 34 (2), 235–243.

[5] Hong, X., Mat Isa, N., Fakurazi, S., and Ismail, I.S., 2020, Phytochemical and anti-inflammatory properties of Scurrula ferruginea (Jack) Danser parasitising on three different host plants elucidated by NMR-based metabolomics, Phytochem. Anal., 31 (1), 15–27.

[6] Rahmad, Z.B., Addo-Fordjour, P., Asyraf, M., and Nik Rosely, N.F., 2014, Mistletoe abundance, distribution and associations with trees along roadsides in Penang, Malaysia, Trop. Ecol., 55 (2), 255–262.

[7] Lohézic-Le Dévéhat, F., Bakhtiar, A., Bézivin, C., Amoros, M., and Boustie, J., 2002, Antiviral and cytotoxic activities of some Indonesian plants, Fitoterapia, 73 (5), 400–405.

[8] Marvibaigi, M., Amini, N., Supriyanto, E., Abdul Majid, F.A., Jaganathan, S.K., Jamil, S., Almaki, J.H., and Nasiri, R., 2016, Antioxidant activity and ROS-dependent apoptotic effect of Scurrula ferruginea (Jack) Danser methanol extract in human breast cancer cell MDA-MB-231, PLoS One, 11 (7), e0158942.

[9] Ameer, O.Z., Salman, I.M., Quek, K.J., and Asmawi, M.Z., 2015, Loranthus ferrugineus: A mistletoe from traditional uses to laboratory bench, J. Pharmacopuncture, 18 (1), 7–18.

[10] Hong, X., Ajat, M., Fakurazi, S., Noor, A.M., and Ismail, I.S., 2021, Anti-inflammatory evaluation of Scurrula ferruginea (Jack) Danser parasitizing on Tecoma stans (L.) H.B.K. in LPS/IFN-γ-induced RAW 264.7 macrophages, J. Ethnopharmacol., 268, 113647.

[11] Justine, V.T., Mustafa, M., and Go, R., 2018, Effect of antimicrobial activities on the various solvents extracts of leaves of Scurrula ferruginea (Jack) Danser (Loranthaceae), Pertanika J. Trop. Agric. Sci., 41 (2), 677–686.

[12] Lohézic-Le Dévéhat, F., Tomasi, S., Fontanel, D., and Boustie, J., 2002, Flavonols from Scurrula ferruginea Danser (Loranthaceae), Z. Naturforsch., C: Biosci., 57 (11-12), 1092–1095.

[13] Harborne, J.B., 1984, Phytochemical Methods - A Guide to Modern Techniques of Plant Analysis, 2nd Ed., Chapman & Hall, London, UK.

[14] Okselni, T., Santoni, A., Dharma, A., and Efdi, M., 2018, Determination of antioxidant activity, total phenolic content, and total flavonoid content of roots, stem bark, and leaves of Elaeocarpus mastersii King, Rasayan J. Chem., 11 (3), 1211–1216.

[15] Truong, D.H., Nguyen, D.H., Ta, N.T.A., Bui, A.V., Do, T.H., and Nguyen, H.C., 2019, Evaluation of the use of different solvents for phytochemical constituents, antioxidants, and in vitro anti-inflammatory activities of Severinia buxifolia, J. Food Qual., 2019, 8178294.

[16] Ezez, D., and Tefera, M., 2021, Effects of solvents on total phenolic content and antioxidant activity of ginger extracts, J. Chem., 2021, 6635199.

[17] Qasim, M., Aziz, I., Rasheed, M., Gul, B., and Ajmal Khan, M., 2016, Effect of extraction solvents on polyphenols and antioxidant activity of medicinal halophytes, Pak. J. Bot., 48 (2), 621–627.

[18] Salamatullah, A.M., Hayat, K., Husain, F.M., Ahmed, M.A., Arzoo, S., Althbiti, M.M., Alzahrani, A., Al-Zaied, B.A.M., Alyahya, H.K., Albader, N., Nafidi, H.A., and Bourhia, M., 2022, Effects of different solvents extractions on total polyphenol content, HPLC analysis, antioxidant capacity, and antimicrobial properties of peppers (red, yellow, and green (Capsicum annum L.)), Evidence-Based Complementary Altern. Med., 2022, 7372101.

[19] Jiang, Z., Kempinski, C., and Chappell, J., 2016, Extraction and analysis of terpenes/terpenoids, Curr. Protoc. Plant Biol., 1 (2), 345–358.

[20] Ahmed, Y., Rahman, S., Akhtar, P., Islam, F., Rahman, M., and Yaakob, Z., 2013, Isolation of steroids from n-hexane extract of the leaves of Saurauia roxburghii, Int. Food Res. J., 20 (5), 2939–2943.

[21] Zubair, M.S., Al-Footy, K.O., Ayyad, S.E.N., Al-Lihaibi, S.S., and Alarif, W.M., 2016, A review of steroids from Sarcophyton species, Nat. Prod. Res., 30 (8), 869–879.

[22] Gutiérrez-Grijalva, E., Picos-Salas, M., Leyva-López, N., Criollo-Mendoza, M., Vazquez-Olivo, G., and Heredia, J., 2017, Flavonoids and phenolic acids from oregano: Occurrence, biological activity and health benefits, Plants, 7 (1), 2.

[23] Markham, K.R., 1989, "Flavones, Flavonols and Their Glycosides" in Methods in Plant Biochemistry: Volume 1: Plant Phenolics, 1st Ed., Eds. Harborne J.B., Academic Press, London, UK, 197–235.

[24] Mabry, T.J., Markham, K.R., and Thomas, M.B., 1970, "The Ultraviolet Spectra of Flavones and Flavonols" in The Systematic Identification of Flavonoids, Springer-Verlag, Berlin, Heidelberg, 41–164.

[25] Aisyah, L.S., Yun, Y.F., Herlina, T., Julaeha, E., Zainuddin, A., Nurfarida, I., Hidayat, A.T., Supratman, U., and Shiono, Y., 2017, Flavonoid compounds from the leaves of Kalanchoe prolifera and their cytotoxic activity against P-388 murine leukimia cells, Nat. Prod. Sci., 23 (2), 139–145.

[26] Zhang, Y., Wang, D., Yang, L., Zhou, D., and Zhang, J., 2014, Purification and characterization of flavonoids from the leaves of Zanthoxylum bungeanum and correlation between their structure and antioxidant activity, PLoS One, 9 (8), e105725.

[27] Gopi, K., Anbarasu, K., Renu, K., Jayanthi, S., Vishwanath, B.S., and Jayaraman, G., 2016, Quercetin-3-O-rhamnoside from Euphorbia hirta protects against snake venom induced toxicity, Biochim. Biophys. Acta, Gen. Subj., 1860 (7), 1528–1540.

[28] Elloumi, W., Mahmoudi, A., Ortiz, S., Boutefnouchet, S., Chamkha, M., and Sayadi, S., 2022, Wound healing potential of quercetin-3-O-rhamnoside and myricetin-3-O-rhamnoside isolated from Pistacia lentiscus distilled leaves in rats model, Biomed. Pharmacother., 146, 112574.

[29] Özbilgin, S., Acıkara, Ö.B., Akkol, E.K., Süntar, I., Keleş, H., and İşcan, G.S., 2018, In vivo wound-healing activity of Euphorbia characias subsp. wulfenii: Isolation and quantification of quercetin glycosides as bioactive compounds, J. Ethnopharmacol., 224, 400–408.

[30] Kaurinovic, B., and Vastag, D., 2019, "Flavonoids and Phenolic Acids as Potential Natural Antioxidants" in Antioxidants, Eds. Shalaby, E., IntechOpen, Rijeka, Croatia.

[31] Marvibaigi, M., Amini, N., Supriyanto, E., Jamil, S., Abdul Majid, F.A., and Khangholi, S., 2014, Total phenolic content, antioxidant and antibacterial properties of Scurrula ferruginea extracts, J. Teknol., 70 (5), 65–72.

[32] Velo-Gala, I., López-Peñalver, J.J., Sánchez-Polo, M., and Rivera-Utrilla, J., 2015, Role of activated carbon on micropollutants degradation by different radiation processes, Mediterr. J. Chem., 4 (2), 68–80.


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