Selective Identification for Glucose in the Presence of Fructose Using Imprinted Polyeugenol Modified Graphite Paste Electrode

https://doi.org/10.22146/ijc.71013

Muhammad Cholid Djunaidi(1*), Gunawan Gunawan(2), Lutfia Cahyaningrum(3), Retno Ariadi Lusiana(4), Miratul Khasanah(5)

(1) Department of Chemistry, Faculty of Science and Mathematics, Diponegoro University, Jl. Prof. Soedharto SH, Tembalang, Semarang 50275, Indonesia
(2) Department of Chemistry, Faculty of Science and Mathematics, Diponegoro University, Jl. Prof. Soedharto SH, Tembalang, Semarang 50275, Indonesia
(3) Department of Chemistry, Faculty of Science and Mathematics, Diponegoro University, Jl. Prof. Soedharto SH, Tembalang, Semarang 50275, Indonesia
(4) Department of Chemistry, Faculty of Science and Mathematics, Diponegoro University, Jl. Prof. Soedharto SH, Tembalang, Semarang 50275, Indonesia
(5) Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Campus C, Jl. Dr. Ir. H. Soekarno (MERR), Surabaya 60115, Indonesia
(*) Corresponding Author

Abstract


High blood glucose levels indicate diabetes mellitus. The conventional way was carried out to determine glucose levels, but this method just shows the total reducing sugar levels. An electrode was developed to analyze glucose in the presence of fructose by potentiometry. The optimum graphite paste electrodes-Imprinted based on polyeugenol and Ethylene Glycol Dimethacrylate (EGDMA) as crosslinkers, had been used as a glucose sensor. Polyeugenol was made by the polymerization reaction with a BF3 catalyst with a molecular weight of 6451.338 g/mol. A total of 2135.99 glucose was contacted with polyeugenol, and the result was crosslinked with EGDMA and 2,2'-Azobis(2-methylpropionitrile) (AIBN) initiator, then Molecularly Imprinted Polymer (MIP) was generated. The optimization of electrodes showed that the best composition of MIP: paraffin: graphite is 20:35:45 with the 19.16 mV/decade Nernstian Factor, which can measure samples at the range 10–5–10–1 M with a detection limit of 1.0334 × 10–5 M, a response time of 6–15 s, a lifetime (use) of 19 times and has good selectivity on fructose sample with Kij less than 1. Measurements using this electrode showed that honey contains 28.78% of glucose, not much different from the UV-Vis spectrophotometry, which is 29.68%, and HPLC is 30.42%.


Keywords


polyeugenol; imprinted; glucose; fructose; selective electrode

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References

[1] Ridwan, A., Astrian, R.T., and Barlian, A., 2012, Pengukuran efek antidiabetes polifenol (Polyphenon 60) berdasarkan kadar glukosa darah dan histologi pankreas mencit (Mus musculus L.) SW jantan yang dikondisikan diabetes mellitus, JMS, 17 (2), 78–82.

[2] McCuistion, L.E., DiMaggio, K.V., Winton, M.B., and Yeager, J.J., 2021, Pharmacology-E-Book: A Patient-Centered Nursing Process Approach, 10th Ed., Elsevier Health Sciences, St. Louis, US.

[3] Djunaidi, M.C., Afriani, M.D.R., Gunawan, G., and Khasanah, M., 2021, Synthesis of graphite paste/molecularly imprinted polymer (MIP) electrodes based on polyeugenol as a glucose sensor with potentiometric method, Indones. J. Chem., 21 (4), 816–824.

[4] Djunaidi, M.C., Khabibi, K., and Ulumudin, I., 2017, Separation of Cu2+, Cd2+ and Cr3+ in a mixture solution using a novel carrier poly(methyl thiazoleethyl eugenoxy acetate) with BLM (Bulk Liquid Membrane), IOP Conf. Ser.: Mater. Sci. Eng., 172, 012032.

[5] BelBruno, J.J., 2018, Molecularly imprinted polymers, Chem. Rev., 119 (1), 94–119.

[6] Saylan, Y., Akgönüllü, S., Yavuz, H., Ünal, S., and Denizli, A., 2019, Molecularly imprinted polymer based sensors for medical applications, Sensors, 19 (6), 1279.

[7] Djunaidi, M.C., 2019, Synthesis, caracterization and selectivity of moleculary imprinted polymer (MIP) glucose using polyeugenol as a functional polymer, Rasayan J. Chem., 12 (2), 809–821.

[8] Djunaidi, M.C., Lusiana, R.A., and Kartikawati, N.G., 2010, Sintesis polieugenol dengan katalis BF3 dietil eter dan H2SO4 untuk ekstraktan logam berat, Prosiding Seminar Nasional Kimia dan Pendidikan Kimia 2010, Badan Penerbit Universitas Diponegoro, 139–145.

[9] Djunaidi, M.C., Prasetya, N.B.A., Khoiriyah, A., Pardoyo, P., Haris, A., and Febriola, N.A., 2020, Polysulfone influence on Au selective adsorbent imprinted membrane synthesis with sulfonated polyeugenol as functional polymer, Membranes, 10 (12), 390.

[10] Shaipulizan, N.S., Md Jamil, S.N.A., Kamaruzaman, S., Subri, N.N.S., Adeyi, A.A., Abdullah, A.H., and Abdullah, L.C., 2020, Preparation of ethylene glycol dimethacrylate (EGDMA)-based terpolymer as potential sorbents for pharmaceuticals adsorption, Polymers, 12(2), 423.

[11] Smith, B.C., 2017, The CO bond, Part I: Introduction and the infrared spectroscopy of alcohols, Spectroscopy, 32 (1), 14–21.

[12] Molina-Fernández, Í., Leuermann, J., Ortega-Moñux, A., Wangüemert-Pérez, J.G., and Halir, R., 2019, Fundamental limit of detection of photonic biosensors with coherent phase read-out, Opt. Express, 27 (9), 12616–12629.

[13] Khasanah, M., Widati, A.A., Handajani, U.S., Harsini, M., Ilmiah, B., and Oktavia, I.D., 2020, Imprinted zeolite modified carbon paste electrode as a selective sensor for blood glucose analysis by potentiometry, Indones. J. Chem., 20 (6), 1301–1310.

[14] Park, S., Boo, H., and Chung, T.D., 2006, Electrochemical non-enzymatic glucose sensors, Anal. Chim. Acta, 556 (1), 46–57.

[15] Ahmed, J., Rashed, M.A., Faisal, M., Harraz, F.A., Jalalah, M., and Alsareii, S.A., 2021, Novel SWCNTs-mesoporous silicon nanocomposite as efficient non-enzymatic glucose biosensor, Appl. Surf. Sci., 552, 149477.

[16] Kim, D.M., Cho, S.J., Cho, C.H., Kim, K.B., Kim, M.Y., and Shim, Y.B., 2016, Disposable all-solid-state pH and glucose sensors based on conductive polymer covered hierarchical AuZn oxide, Biosens. Bioelectron., 79, 165–172.

[17] Alhans, R., Singh, A., Singhal, C., Narang, J., Wadhwa, S., and Mathur, A., 2018, Comparative analysis of single-walled and multi-walled carbon nanotubes for electrochemical sensing of glucose on gold printed circuit boards, Mater. Sci. Eng., C, 90, 273–279.

[18] Son, S.G., Park, H.J., Kim, Y.K., Cho, H.S., and Choi, B.G., 2019, Fabrication of low-cost and flexible potassium ion sensors based on screen printing and their electrochemical characteristics, Appl. Chem. Eng., 30 (6), 737–741.

[19] Tohda, K., Dragoe, D., Shibata, M., and Umezawa, Y., 2001, Studies on the matched potential method for determining the selectivity coefficients of ion-selective electrodes based on neutral ionophores: Experimental and theoretical verification, Anal. Sci., 17 (6), 733–743.

[20] Catrall, R.W., 1997, Chemical Sensors, Oxford University Press, United Kingdom.



DOI: https://doi.org/10.22146/ijc.71013

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