Bioactive Secondary Metabolites from the Endophytic Fungi  Alternaria  sp.

Antonius Rolling Basa Ola; Dodi Darmakusuma; Luther Kadang; Amor Tresna Karyawati; Sherly Monitha Febriani Ledoh; Imanuel Gauru; Pius Dore Ola; Suwari Suwari; Henderiana Laura Loiusa Belli

doi:10.22146/ijc.68922

Bioactive Secondary Metabolites from the Endophytic Fungi Alternaria sp.

https://doi.org/10.22146/ijc.68922

Antonius Rolling Basa Ola^(1*), Dodi Darmakusuma⁽²⁾, Luther Kadang⁽³⁾, Amor Tresna Karyawati⁽⁴⁾, Sherly Monitha Febriani Ledoh⁽⁵⁾, Imanuel Gauru⁽⁶⁾, Pius Dore Ola⁽⁷⁾, Suwari Suwari⁽⁸⁾, Henderiana Laura Loiusa Belli⁽⁹⁾

(1) Department of Chemistry, Faculty of Science and Engineering, Universitas Nusa Cendana, Jl. Adisucipto, Penfui-Kupang 85118, NTT, Indonesia; Integrated Research Center (Bioscience) Laboratory, Nusa Cendana University, Jl. Adisucipto, Penfui-Kupang 85118, NTT, Indonesia
(2) Department of Chemistry, Faculty of Science and Engineering, Universitas Nusa Cendana, Jl. Adisucipto, Penfui-Kupang 85118, NTT, Indonesia
(3) Department of Chemistry, Faculty of Science and Engineering, Universitas Nusa Cendana, Jl. Adisucipto, Penfui-Kupang 85118, NTT, Indonesia
(4) Department of Biology, Faculty of Science and Engineering, Nusa Cendana University, Jl. Adisucipto, Penfui-Kupang 85118, NTT, Indonesia
(5) Department of Chemistry, Faculty of Science and Engineering, Universitas Nusa Cendana, Jl. Adisucipto, Penfui-Kupang 85118, NTT, Indonesia
(6) Department of Chemistry, Faculty of Science and Engineering, Universitas Nusa Cendana, Jl. Adisucipto, Penfui-Kupang 85118, NTT, Indonesia
(7) Department of Chemistry, Faculty of Science and Engineering, Universitas Nusa Cendana, Jl. Adisucipto, Penfui-Kupang 85118, NTT, Indonesia
(8) Department of Chemistry, Faculty of Science and Engineering, Universitas Nusa Cendana, Jl. Adisucipto, Penfui-Kupang 85118, NTT, Indonesia
(9) Integrated Research Center (Bioscience) Laboratory, Nusa Cendana University, Jl. Adisucipto, Penfui-Kupang 85118, NTT, Indonesia
(*) Corresponding Author

Abstract

Shifting the drug discovery from plants to microorganisms due to minimizing the ecological effects has led to the increasing work on finding bioactive metabolites from endophytic fungi, especially those isolated from medicinal plants growing in arid and semi-arid land. An endophytic fungi Alternaria sp. isolated from dry land was cultivated on solid rice media and then extracted with ethyl acetate. From the fungal ethyl acetate crude extract of Alternaria sp., compounds altertoxin I (1) and II (2), and stemphyltoxin III (3), were isolated based on the bioactivity assay. The structures of compounds 1-3 were identified using UV, NMR, and mass spectral data and compared to those spectral data reported in the literature. The absolute configuration was determined to be the same as the previous report based on the comparison with the optical rotation value. The three compounds showed strong cytotoxic activity, with altertoxin II being the most active ones having the inhibition of growth observed at 94.4% against murine lymphoma L5178Y cell line at 10 µg/mL. The activity of altertoxin II (2) was probably contributed by the epoxytetralone moiety. The finding of this study still confirms the importance of endophytic fungi as sources of bioactive molecules.

Keywords

endophytic fungi; cytotoxic; altertoxin I and II; stemphyltoxin III

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DOI: https://doi.org/10.22146/ijc.68922