Metabolite Profiling of Ebony ( Diospyros celebica  Bakh) Leaves and Wood Extracts Using LC-MS/MS

Dien Atin Boritnaban; Alfi Hudatul Karomah; Dewi Anggraini Septaningsih; Muhammad Majiidu; Fifi Gus Dwiyanti; Iskandar Zulkarnaen Siregar; Mohamad Rafi

doi:10.22146/ijc.68529

Metabolite Profiling of Ebony (Diospyros celebica Bakh) Leaves and Wood Extracts Using LC-MS/MS

https://doi.org/10.22146/ijc.68529

Dien Atin Boritnaban⁽¹⁾, Alfi Hudatul Karomah⁽²⁾, Dewi Anggraini Septaningsih⁽³⁾, Muhammad Majiidu⁽⁴⁾, Fifi Gus Dwiyanti⁽⁵⁾, Iskandar Zulkarnaen Siregar⁽⁶⁾, Mohamad Rafi^(7*)

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Jl. Tanjung Kampus IPB Dramaga, Bogor 16680, Indonesia
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Jl. Tanjung Kampus IPB Dramaga, Bogor 16680, Indonesia
(3) Advanced Research Laboratory, Institute of Research and Community Services, IPB University, Jl. Palem Raya Kampus IPB Dramaga, Bogor 16680, Indonesia
(4) Advanced Research Laboratory, Institute of Research and Community Services, IPB University, Jl. Palem Raya Kampus IPB Dramaga, Bogor 16680, Indonesia
(5) Advanced Research Laboratory, Institute of Research and Community Services, IPB University, Jl. Palem Raya Kampus IPB Dramaga, Bogor 16680, Indonesia Department of Silviculture, Faculty of Forestry and Environment, IPB University, Jl. Lingkar Akademik Kampus IPB Dramaga, Bogor 16680, Indonesia
(6) Advanced Research Laboratory, Institute of Research and Community Services, IPB University, Jl. Palem Raya Kampus IPB Dramaga, Bogor 16680, Indonesia Department of Silviculture, Faculty of Forestry and Environment, IPB University, Jl. Lingkar Akademik Kampus IPB Dramaga, Bogor 16680, Indonesia
(7) Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Jl. Tanjung Kampus IPB Dramaga, Bogor 16680, Indonesia Advanced Research Laboratory, Institute of Research and Community Services, IPB University, Jl. Palem Raya Kampus IPB Dramaga, Bogor 16680, Indonesia Tropical Biopharmaca Research Center, Institute of Research and Community Services, IPB University, Jl. Taman Kencana No. 3, Bogor 16128, Indonesia
(*) Corresponding Author

Abstract

Ebony (Diospyros celebica Bakh) is an endemic forest tree species found in Sulawesi whose basic information on its metabolite profile is still lacking. The objective of this research was to separate and identify putatively metabolite present in the leaves and wood of ebony across Sulawesi. Separation and identification of ebony metabolites were carried out using UHPLC-Q-Orbitrap HRMS analysis. Using ultrasonication with ethanol as the extracting solvent, we used powdered ebony leaves and wood. The results showed that the metabolites contained in the leaves and wood of ebony were 59 metabolites. About 14 compounds were found in the leaves and wood, 21 compounds in the wood, and 24 compounds in the leaves. The identified metabolites are flavonoids, terpenoids, amino aldehydes, alkaloids, quinones, steroids, amino acids, fatty acids, and saccharides. Clustering of ebony using principal component analysis obtained leaves and wood groups using peak area of known compounds as the variable.

Keywords

Diospyros celebica Bakh; ebony; metabolomics; UHPLC-Q-Orbitrap HRMS

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References

[1] Karlinasari, L., Noviyanti, N., Purwanto, Y.A., Majiidu, M., Dwiyanti, F.G., Rafi, M., Damayanti, R., Harnelly, E., and Siregar, I.Z., 2021, Discrimination and determination of extractive content of ebony (Diospyros celebica Bakh.) from Celebes sland by near-infrared spectroscopy, Forests, 12 (1), 6.

[2] Yuniarti, N., Syamsuwida, D., and Aminah, A., 2012, Dampak perubahan fisiologi dan biokimia benih eboni (Diospyros celebica Bakh) selama penyimpanan, Jurnal Penelitian Hutan Tanaman, 10 (2), 65–71.

[3] Arianti, A., Budiarso, E., Budi, A.S., and Kusuma, I.W., 2016, Analisis fitokimia ekstrak kayu eboni (Diospyros celebica Bakh), Warta Rimba, 4 (2), 61–68.

[4] Kartini, K., Khumaidi, A., Khaerati, K., and Ihwan, I., 2018, Ekstrak etanol daun eboni menurunkan kadar glukosa darah tikus jantan yang diinduksi aloksan, Jurnal Veteriner., 19 (3), 329–334.

[5] Nugrahaini, A.W., Maulida, M.F., Khumaidi, A., 2020, Aktivitas antibakteri fraksi serbuk kayu eboni (Diospyros celebica Bakh.) terhadap Escherichia coli dan Staphylococcus aureus, J. Sains Farm. Klin., 7 (3), 194–201.

[6] Chen, J., Ni, C., Lou, J., and Peng, W., 2018, Molecules and functions of rosewood: Diospyros celebica, Arabian J. Chem., 11 (6), 756–762.

[7] Yang, Q., Zhang, A., Miao, J., Sun, H., Han, Y., Yan, G., Wu, F., and Wang, X., 2019, Metabolomics biotechnology, applications, and future trends: A systematic review, RSC Adv., 9 (64), 37245–37257.

[8] Zeki, Ö.C., Eylem, C.C., Reçber, T., Kır, S., and Nemutlu, E., 2020, Integration of GC-MS and LC-MS for untargeted metabolomics profiling, J. Pharm. Biomed. Anal., 190, 113509.

[9] Fan, M., Lian, W., Li, T., Fan, Y., Rao, Z., Li, Y., Qian, H., Zhang, H., Wu, G., Qi, X., and Wang, L., 2020, Metabolomics approach reveals discriminatory metabolites associating with the blue pigments from Vaccinium bracteatum thunb. leaves at different growth stages, Ind. Crops Prod., 147, 112252.

[10] Rafi, M., Karomah, A.H., Heryanto, R., Septaningsih, D.A., Kusuma, W.A., Amran, M.B., Rohman, A., and Prajogo, B., 2022, Metabolite profiling of Andrographis paniculata leaves and stem extract using UHPLC-Orbitrap-MS/MS, Nat. Prod. Res., 36 (2), 625–629.

[11] Baloglu, M.C., Llorent-Martínez, E.J., Aumeeruddy, M.Z., Mahomoodally, M.F., Altunoglu, Y.C., Ustaoglu, B., Ocal, M., Gürel, S., Bene, K., Sinan, K.I., and Zengin, G., 2019, Multidirectional insights on Chrysophyllum perpulchrum leaves and stem bark extracts: HPLC-ESI-MSⁿ profiles, antioxidant, enzyme inhibitory, antimicrobial and cytotoxic properties, Ind. Crops Prod., 134, 33–42.

[12] Llorent-Martinez, E.J., Ruiz-Riaguas, A., Sinan, K.I., Bene, K., Cordova, M.L.F., Picot-Allain, C., Mahomoodally, F., Saleem, H., and Zengin, G., 2020, Exploring chemical profiles and bioactivities of Harungana madagascariensis Lam. ex Poir. leaves and stem bark extracts: A new source of procyanidins, Anal. Lett., 53 (3), 399–412.

[13] Sun, K., Su, C., Li, W., Gong, Z., Sha, C., and Liu, R., 2021, Quality markers based on phytochemical analysis and anti-inflammatory screening: An integrated strategy for the quality control of Dalbergia odorifera by UHPLC-Q-Orbitrap HRMS, Phytomedicine, 84, 153511.

[14] Chin, S., Behm, C., and Mathesius, U., 2018, Functions of flavonoids in plant-nematode interactions, Plants, 7 (4), 85.

[15] Tholl, D., 2015, Biosynthesis and biological functions of terpenoids in plants, Adv. Biochem. Eng./Biotechnol., 148, 63–106.

[16] Bjerrum, J.T., Wang, Y., Hao, F., Coskun, M., Ludwig, C., Günther, U., and Nielsen, O.H., 2015, Metabonomics of human fecal extracts characterize ulcerative colitis, Crohn's disease and healthy individuals, Metabolomics, 11 (1), 122–133.

[17] Ponnusamy, K., Choi, J.N., Kim, J., Lee, S.Y., and Lee, C.H., 2011, Microbial community and metabolomic comparison of irritable bowel syndrome faeces, J. Med. Microbiol., 60 (6), 817–827.

[18] Eschen-Lippold, L., Rothe, G., Stumpe, M., Göbel, C., Feussner, I., and Rosahl, S., 2007, Reduction of divinyl ether-containing polyunsaturated fatty acids in transgenic potato plants, Phytochemistry, 68 (6), 797–801.

[19] Ďuriš, V., Bartková, R., and Tirpáková, A. 2021, Principal component analysis and factor analysis for an Atanassov IF data set, Mathematics, 9 (17), 2067.

[20] International Council for Harmonisation, 1995, Validation of Analytical Procedures: Text and methodology Q2(R1), ICH Harmonised Tripartite Guideline, CPMP/ICH/381/95, Geneva.

DOI: https://doi.org/10.22146/ijc.68529