The Biotransformation and Biodecolorization of Methylene Blue by Xenobiotic Bacterium Ralstonia pickettii

https://doi.org/10.22146/ijc.65806

Adi Setyo Purnomo(1*), Asranudin Asranudin(2), Didik Prasetyoko(3), Yulinar Dwi Nur Azizah(4)

(1) Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember (ITS), Kampus ITS Sukolilo, Surabaya 60111, Indonesia
(2) Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember (ITS), Kampus ITS Sukolilo, Surabaya 60111, Indonesia
(3) Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember (ITS), Kampus ITS Sukolilo, Surabaya 60111, Indonesia
(4) Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember (ITS), Kampus ITS Sukolilo, Surabaya 60111, Indonesia
(*) Corresponding Author

Abstract


The biotransformation and biodecolorization of methylene blue (MB) dye using the bacterium Ralstonia pickettii was investigated. This experiment was conducted in a nutrient broth (NB) medium after adding MB at 100 mg L–1 concentration. Approximately 98.11% of MB was decolorized after 18 h of incubation. In addition, the metabolic products detected by LC-TOF/MS were Azure A (AA), thionine, leuco-MB, and glucose-MB, which indicated the MB degradation through a reductase that attacked the heterocyclic central chromophore group present in the structure. Moreover, azure A and thionine fragments resulted from the N-demethylase enzyme that attacked the auxochrome group. Thus, this research was assumed to be the first scientific report suggesting the potential to use R. pickettii in the biodecolorization and biotransformation of dye waste, particularly MB.

Keywords


decolorization; biotransformation; xenobiotic bacteria; Ralstonia pickettii; methylene blue

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References

[1] Tkaczyk, A., Mitrowska, K., and Posyniak, A., 2020. Synthetic organic dyes as contaminants of the aquatic environment and their implications for ecosystems: A review, Sci. Total Environ., 717, 137222.

[2] Bommavaram, K., Bhattacharjee, A., Yadav, D., Andra, N., Pandey, P., and Ibrahim, H., 2020, Tea residue as a bio-sorbent for the treatment of textile industry effluents, Int. J. Environ. Sci. Technol., 17 (7), 3351–3364.

[3] Abu-Talha, M., Goswami, M., Giri, B.S., Sharma, A., Rai, B.N., and Singh, R.S., 2018, Bioremediation of Congo red dye in immobilized batch and continuous packed bed bioreactor by Brevibacillus parabrevis using coconut shell biochar, Bioresour. Technol., 252, 37–43.

[4] Purnomo, A.S., and Mawaddah, M.O., 2020, Biodecolorization of methyl orange by mixed cultures of brown-rot fungus Daedalea dickinsii and bacterium Pseudomonas aeruginosa, Biodiversitas, 21 (5), 2297–2302.

[5] Geed, S.R., Samal. K., and Tagade, A., 2019, Development of adsorption-biodegradation hybrid process for removal of methylene blue from wastewater, J. Environ. Chem. Eng., 7 (6), 103439.

[6] Zhou, Y., Lu, J., Zhou, Y., and Liu, Y., 2019, Recent advances for dyes removal using novel adsorbents: A review, Environ. Pollut., 252, 352–365.

[7] Shah, M.P., 2019, Chapter 6 - Bioremediation of azo dye” in Microbial Wastewater Treatment, Eds. Shah, M.P., and Rodriguez-Couto, S., Elsevier, Netherlands, 103–126.

[8] Zaini, M.A.A., Ngiik, T.C., Kamaruddin, M.J., Mohd. Setapar, S.M., and Che Yunus, M.A., 2014, Zinc chloride-activated waste carbon powder for decolourization of Methylene blue, Jurnal Teknologi, 67 (2), 37–44.

[9] Ehrampoush, M.H., Moussavi, G.R., Ghaneian, M.T., Rahimi, S., and Ahmadian, M., 2011, Removal of methylene blue dye from textile simulated sample using tubular reactor and TiO2/UV-C photocatalytic process, Iran. J. Environ. Health Sci. Eng., 8 (1), 35–40.

[10] Kurade, M.B., Waghmode, T.R., Xiong, J.Q., Govindwar, S.P., and Jeon, B.H., 2019, Decolorization of textile industry effluent using immobilized consortium cells in upflow fixed bed reactor, J. Cleaner Prod., 213, 884–891.

[11] Xiong, J., Guo, S., Zhao, T., Liang, Y., Liang, J., Wang, S., Zhu, H., Zhao, J.R., and Chen, G., 2020, Degradation of methylene blue by intimate coupling photocatalysis and biodegradation with bagasse cellulose composite carrier, Cellulose, 27 (6), 3391–3404.

[12] Boelan, E.G., and Purnomo, A.S., 2018, Abilities of Co-cultures of white-rot fungus Ganoderma lingzhi and bacteria Bacillus subtilis on biodegradation DDT, J. Phys.: Conf. Ser., 1095, 012015.

[13] Sariwati, A., Purnomo, A.S., and Kamei I., 2017, Abilities of co-cultures of brown-rot fungus Fomitopsis pinicola and Bacillus subtilis on biodegradation of DDT, Curr. Microbiol., 74 (9), 1068–1075.

[14] Li, H, Zhang, R, Tang, L., Zhang, J., and Mao, Z., 2014, Evaluation of Bacillus sp. MZS10 for decolorizing Azure B dye and its decolorization mechanism, J. Environ. Sci., 26 (5), 1125–1134.

[15] Schirmer, R.H., Adler, H., Pickhardt, M., and Mandelkow, E., 2011, Lest we forget you -methylene blue, Neurobiol. Aging, 32 (12), 2325.e7–2325.e16.

[16] Rizqi, H.D., and Purnomo, A.S., 2017, The ability of brown-rot fungus Daedalea dickinsii to decolorize and transform methylene blue dye, World J. Microbiol. Biotechnol., 33 (5), 92.

[17] Zhou, S., Du, Z., Li, X., Zhang, Y., He, Y., and Zhang, Y., 2019, Degradation of methylene blue by natural manganese oxides: Kinetics and transformation products, R. Soc. Open Sci., 6 (7), 190351.

[18] Purnomo, A.S., Rizqi, H.D., Fatmawati, S., Putro, H.S., and Kamei, I., 2018, Effects of bacterium Ralstonia pickettii addition on DDT biodegradation by Daedalea dickinsii, Res. J. Chem. Environ., 22, 151–156.

[19] Upendar, G., Dutta, S., Bhattacharya, P., and Dutta, A., 2017, Bioremediation of methylene blue dye using Bacillus subtilis MTCC 441, Water Sci. Technol., 75 (7), 1572–1583.

[20] Habibi, A., and Mehrabi, Z., 2017, Aerobic degradation of methylene blue from colored effluents by Ralstonia eutropha, Pollution, 3 (3), 395–406.

[21] Bharti, V., Vikrant, K., Tiwari, H., Sonwani, R.K., Lee, J., Tsang, D.C.W., Kim, K.H., Saeed, M., Kumar, S., Rai, B.N., Giri, B.S., and Singh, R.S., 2019, Biodegradation of methylene blue dye in a batch and continuous mode using biochar as packing media, Environ. Res., 171, 356–364.

[22] Bankole, P.O., Adekunle, A.A., and Govindwar, S.P., 2019, Demethylation and desulfonation of textile industry dye, thiazole yellow G by Aspergillus niger LAG, Biotechnol. Rep., 23, e00327.

[23] Maniyam, M.N., Hari, M., and Yaacob, N.S., 2020, Enhanced methylene blue decolourization by Rhodococcus strain UCC 0003 grown in banana peel agricultural waste through response surface methodology, Biocatal. Agric. Biotechnol., 23, 101486.

[24] Ryan, M.P., Pembroke, J.T., and Adley, C.C., 2011, Genotypic and phenotypic diversity of Ralstonia pickettii and Ralstonia insidiosa isolates from clinical and environmental sources including high-purity water, diversity in Ralstonia pickettii, BMC Microbiol., 11 (1), 194.

[25] Byrne, A.M., and Olsen, R.H., 1996, Cascade regulation of the toluene-3-monooxygenase operon (tbuA1UBVA2C) of Burkholderia pickettii PKO1: Role of the tbuA1 promoter (PtbuA1) in the expression of its cognate activator, TbuT, J. Bacteriol., 178 (21), 6327–6337.

[26] Purnomo, A.S., Rizqi, H.D., Harmelia, L., Anggraeni, S.D., Melati, R.E., Damayanti, Z.H., Shafwah, O.M., and Kusuma, F.C., 2019, Biodegradation of crude oil by Ralstonia pickettii under high salinity medium, MJFAS, 15 (3), 377–380.

[27] Al-Zuhair, S., and El-Naas, M.H., 2012, Phenol biodegradation by Ralstonia pickettii extracted from petroleum refinery oil sludge, Chem. Eng. Commun., 199 (9), 1194–1204.

[28] Zhang, L.L., Leng, S.Q., Zhu, R.Y., and Chen, J.M., 2011, Degradation of chlorobenzene by strain Ralstonia pickettii L2 isolated from a biotrickling filter treating a chlorobenzene-contaminated gas stream, Appl. Microbiol. Biotechnol., 91 (2), 407–415.

[29] Ryan, M.P., Pembroke, J.T., and Adley, C.C., 2007, Ralstonia pickettii in environmental biotechnology: Potential and applications, J. Appl. Microbiol., 103 (4), 754–764.

[30] Purnomo, A.S., Sariwati, A., and Kamei, I., 2020, Synergistic interaction of a consortium of the brown-rot fungus Fomitopsis pinicola and the bacterium Ralstonia pickettii for DDT biodegradation, Heliyon., 6 (6), e04027.

[31] Wahyuni, S., Suhartono, M.T., Khaeruni, A., Purnomo, A.S., Asranudin, Holilah, and Riupassa, P.A., 2016, Purification and characterization of thermostable chitinase from Bacillus SW41 for chitin oligomer production, Asian J. Chem., 28 (12), 2731–2736.

[32] Khan, R., Patel, V., and Khan, Z., 2020, “Chapter 5 – Bioremediation of Dyes from Textile and Dye Manufacturing Industry Effluent” in Abatement of Environmental Pollutants Trends and Strategies, Eds. Singh, P., Kumar, A., and Borthakur, A., Elsevier, Netherlands, 107–125.

[33] Maniyam, M.N., Yaacob, N.S., Azman, H.H., Ab Ghaffar, N.A., and Abdullah, H., 2018, Immobilized cells of Rhodococcus strain UCC 0004 as source of green biocatalyst for decolourization and biodegradation of methyl orange, Biocatal. Agric. Biotechnol., 16, 569–578.

[34] Zeng, G., Cheng, M., and Huang, D., 2015, Study of the degradation of methylene blue by semi-solid-state fermentation of agricultural residues with Phanerochaete chrysosporium and reutilization of fermented residues, Waste Manage., 38, 424–430.

[35] Michelle, Siregar, R.A.N., Sanjaya, A., Lucy, J., and Pinontoan, R., 2020, Methylene blue decolorizing bacteria isolated from water sewage in Yogyakarta, Indonesia, Biodiversitas, 21 (3), 1136–1141.

[36] Jayasinghe, C., Imtiaj, A., Lee, G.W., Im, K.H., Hur, H., Lee, M.W., Yang, H.S., and Lee, T.S., 2008, Degradation of three aromatic dyes by white rot fungi and the production of ligninolytic enzymes, Mycobiology, 36 (2), 114–120.

[37] Singh, S.N., 2015, Microbial Degradation of Synthetic Dyes in Wastewaters, Springer Nature, Switzerland.

[38] Liu, Y.N., Zhou, X., Wang, X., Liang, K., Yang, Z.K., Shen, C.C., Imran, M., Sahar, S., and Xu, A.W., 2017, Hydrogenation/oxidation induced efficient reversible color switching between methylene blue and leuco-methylene blue, RSC Adv., 7, 30080–30085.

[39] Singh, R.L., Singh, P.K., and Singh, R.P., 2015, Enzymatic decolorization and degradation of azo dyes - A review, Int. Biodeterior. Biodegrad., 104, 21–31.

[40] Purnomo, A.S., Rizqi, H.D., Harmelia, L., Anggraeni, S.D., Melati, R.E., Damayanti, Z.H., Shafwah, O.M., and Kusuma, F.C., 2019, Biodegradation of crude oil by Ralstonia pickettii under high salinity medium, MJFAS, 15 (3), 377–380.

[41] Purnomo, A.S., Maulianawati, D., and Kamei, I., 2019, Ralstonia pickettii enhance the DDT biodegradation by Pleurotus eryngii, J. Microbiol. Biotechnol., 29, 1424–1433.

[42] Deska, M., and Kończak, B., 2019, Immobilized fungal laccase as “green catalyst” for the decolourization process – State of the art, Process Biochem., 84, 112–123.

[43] Misal, S.A., and Gawai K.R., 2018, Azoreductase: A key player of xenobiotic metabolism, Bioresour. Bioprocess., 5 (1), 17.

[44] Xu, L., Sun, J., Qaria, M.A., Gao, L., and Daochen, Z., 2021, Dye decoloring peroxidase structure, catalytic properties and applications: Current advancement and futurity, Catalysts, 11 (8), 955.

[45] Yang, C., Dong, W., Cui, G., Zhao, Y., Shi, X., Xia, X., Tang, B., and Wang, W., 2017, Highly efficient photocatalytic degradation of synergetic effect of TiO2 and P2ABSA, RS Adv., 7 (38), 23699–23708.

[46] Trandafilović, L.V., Jovanović, D.J., Zhang, X., Ptasińska, S., and Dramićanin, M.D., 2017, Enhanced photocatalytic degradation of methylene blue and methyl orange by ZnO:Eu nanoparticles, Appl. Catal., B, 203, 740–752.

[47] Kurade, M.B., Waghmode, T.R., Kagalkar, A.N., and Govindwar, S.P., 2012, Decolorization of textile industry effluent containing disperse dye Scarlet RR by a newly developed bacterial-yeast consortium BL-GG, Chem. Eng. J., 184, 33–41.

[48] Bandounas, L., Pinkse, M., de Winde, J.H., and Ruijssenaars, H.J., 2013, Identification of a quinone dehydrogenase from a Bacillus sp. involved in the decolourization of the lignin-model dye, Azure B, New Biotechnol., 30 (2), 196–204.

[49] Dhanve, R.S., Kalyani, D.C., Phugare, S.S., and Jadhav, J.P., 2009, Coordinate action of exiguobacterial oxidoreductive enzymes in biodegradation of reactive yellow 84A dye, Biodegradation., 20 (2), 245–255.

[50] Jadhav, S.B., Patil, N.S., Watharkar, A.D., Apine, O.A., and Jadhav, J.P., 2013, Batch and continuous biodegradation of Amaranth in plain distilled water by P. aeruginosa BCH and toxicological scrutiny using oxidative stress studies, Environ. Sci. Pollut. Res., 20 (5), 2854–2866.

[51] Ferreira, V.S., Magalhães, D.B., and Kling, S.H., 2000, N-demethylation of methylene blue by lignin peroxidase from Phanerochaete chrysosporium. Stoichiometric relation for H2O2 consumption, Appl. Biochem. Biotechnol., 84-86, 255–265.

[52] Griffin, B.W., and Ting, P.L., 1978, Mechanism of N-demethylation of aminopyrine by hydrogen peroxide catalyzed by horseradish peroxidase, metmyoglobin, and protohemin, Biochemistry, 17 (11), 2206–2211.

[53] Kalme, S.D., Parshetti, G.K., Jadhav, S.U., and Govindwar, S.P., 2007, Biodegradation of benzidine based dye Direct Blue-6 by Pseudomonas desmolyticum NCIM 2112, Bioresour. Technol., 98 (7), 1405–1410.

[54] Kalyani, D.C., Patil, P.S., Jadhav, J.P., and Govindwar, S.P., 2008, Biodegradation of reactive textile dye Red BLI by an isolated bacterium Pseudomonas sp. SUK1, Bioresour. Technol., 99 (11), 4635–4641.

[55] Dawkar, V.V., Jadhav, U.U., Jadhav, S.U., and Govindwar, S.P., 2008, Biodegradation of disperse textile dye Brown 3REL by newly isolated Bacillus sp. VUS, J. Appl. Microbiol., 105 (1), 14–24.

[56] Bumpus, A., and Brock, B.J., 1988, Biodegradation of crystal violet by the white rot fungus Phanerochaete chrysosporium, Appl. Environ. Microbiol., 54 (5), 1143–1150.

[57] Chen, C.H., Chang, C.F., Ho, C.H., Tsai, T.L., and Liu, S.M., 2008, Biodegradation of crystal violet by a Shewanella sp. NTOU1, Chemosphere, 72 (11), 1712–1720.

[58] Wang, Q., Tian, S., and Ning, P., 2013, Degradation mechanism of methylene blue in a heterogeneous Fenton-like reaction catalyzed by ferrocene, Ind. Eng. Chem. Res., 53 (2), 643–649.



DOI: https://doi.org/10.22146/ijc.65806

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