Selective Solid-Phase Extraction of Meropenem from Human Blood Plasma Using a Molecularly Imprinted Polymer

Lasmaryna Sirumapea(1*), Mohammad Ali Zulfikar(2), Muhammad Bachri Amran(3), Anita Alni(4)

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bandung Institute of Technology, Jl. Ganesha No. 10, Bandung 40132, West Java, Indonesia; STIFI Bhakti Pertiwi Palembang, Jl. Ariodillah III No. 22, Palembang 30128, South Sumatera, Indonesia
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bandung Institute of Technology, Jl. Ganesha No. 10, Bandung 40132, West Java, Indonesia
(3) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bandung Institute of Technology, Jl. Ganesha No. 10, Bandung 40132, West Java, Indonesia
(4) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bandung Institute of Technology, Jl. Ganesha No. 10, Bandung 40132, West Java, Indonesia
(*) Corresponding Author


This study employed a selective and high adsorption performance for meropenem. Molecularly imprinted polymer for meropenem (MeIP) as the selective sorbent was prepared through a bulk polymerization reaction. Methacrylic acid, ethylene glycol dimethacrylate, benzoyl peroxide, and dimethyl sulfoxide were applied as functional monomer, crosslinker agent, initiator, and solvent, respectively. Scanning electron microscopy, thermogravimetric analysis, Brunauer-Emmett-Teller analysis, and Fourier transform infrared spectroscopy were used to characterize the morphology, pore size, and structure of imprinted polymers. The maximum adsorption capacity was achieved at pH = 3, after 4 h contacted, under 150 rpm, and 25 mg of polymer applied. The maximum adsorption capacity of MeIP for meropenem was 51.963 mg/L; the synthesized polymer had superior selectivity to meropenem compared to that of the other antibiotics (imprinting factor, IF = 2.58). Furthermore, the thermodynamic and kinetic analyses indicated that the results were in accord with the Freundlich model and the pseudo-second-order kinetic model, respectively. MeIP was selective in batch adsorption, and molecularly imprinted solid-phase extraction protocols were selective to meropenem. It was then applied to analyze meropenem in human blood plasma and resulted in 78.52 ± 2.71 of recovery.


meropenem; molecularly imprinted polymer; selective; solid-phase extraction

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[1] Mendez, A.S.L., Steppe, M., and Schapoval, E.E.S., 2003, Validation of HPLC and UV spectrophotometric methods for the determination of meropenem in pharmaceutical dosage form, J. Pharm. Biomed. Anal., 33 (5), 947–954.

[2] Baldwin, C.M., Lyseng-Williamson, K.A., and Keam, S.J., 2008, Meropenem: A review of its use in the treatment of serious bacterial infections, Drugs, 68 (6), 803–838.

[3] Nicolau, D.P., 2008, Pharmacokinetic and Pharmacodynamic of Meropenem, Clin. Infect. Dis., 47 (Suppl. 1), S32–S40.

[4] Beltran, A., Marcé, R.M., Cormack, P.A.G., Sherrington, D.C., and Borrull, F., 2008, Selective solid‐phase extraction of amoxicillin and cephalexin from urine samples using a molecularly imprinted polymer, J. Sep. Sci., 31 (15), 2868–2874.

[5] Tuerk, J., Reinders, M., Dreyer, D., Kiffmeyer, T.K., Schmidt, K.G., and Kuss, H.M., 2006, Analysis of antibiotics in urine and wipe samples from environmental and biological monitoring–Comparison of HPLC with UV-, single MS- and tandem MS-detection, J. Chromatogr. B, 831 (1), 72–80.

[6] Soran, M.L., Lung, I., Opriş, O., Floare-Avram, V., and Coman, C., 2017, Determination of antibiotics in surface water by solid-phase extraction and high-performance liquid chromatography with diode array and mass spectrometry detection, Anal. Lett., 50 (7), 1209–1218.

[7] Oyedeji, A.O., Msagati, T.A.M., Williams, A.B., and Benson, N.U., 2020, Solid-phase extraction and high performance liquid chromatography with diode array detection method for the determination of antibiotic residues in poultry tissues, Chem. Data Collect., 25, 100312.

[8] Amlashi, H.S., Daryasari, A.P., and Soleimani, M., 2019, Molecularly imprinted polymer solid phase extraction followed by high-performance liquid chromatography as an efficient and sensitive technique for determination of meropenem in human plasma and urine, S. Afr. J. Chem., 72, 32–39.

[9] Chow, A.L.J., and Bhawani, S.A., 2016, Synthesis and characterization of molecular imprinting polymer microspheres of cinnamic acid: Extraction of cinnamic acid from spiked blood plasma, Int. J. Polym. Sci., 2016, 2418915.

[10] Duan, Z.J., Fan, L.P., Fang, G.Z., Yi, J.H., and Wang, S., 2011, Novel surface molecularly imprinted sol–gel polymer applied to the online solid phase extraction of methyl-3-quinoxaline-2-carboxylic acid and quinoxaline-2-carboxylic acid from pork muscle, Anal. Bioanal. Chem., 401 (7), 2291–2299.

[11] Kryscio, D.R., and Peppas, N.A., 2012, Surface imprinted thin polymer film systems with selective recognition for bovine serum albumin, Anal. Chim. Acta, 718, 109–115.

[12] Du, W., Zhou, H., Luo, Z., Zheng, P., Guo, P., Chang, R., Chang, C., and Fu, Q., 2014, Selective determination of penicillin G from tap water and milk samples using surface molecularly imprinted polymers as solid-phase extraction sorbent, Mol. Imprinting, 2 (1), 18–29.

[13] Yan, H., and Row, K.H., 2006, Characteristic and synthetic approach of molecularly imprinted polymer, Int. J. Mol. Sci., 7 (5), 155–178.

[14] Jin, Y.F., Zhang, Y.J., Zhang, Y.P., Chen, J., Zhou, X.M., and Bai, L.Y., 2013, Synthesis and evaluation of molecularly imprinting polymer for the determination of the phthalate esters in the bottled beverages by HPLC, J. Chem., 2013, 903210.

[15] Cielecka-Piontek, J., Paczkowska, M., Lewandowska, K., Barszcz, B., Zalewski, P., and Garbacki, P., 2013, Solid-state stability study of meropenem – solutions based on spectrophotometric analysis, Chem. Cent. J., 7, 98.

[16] Barros, L.A., Custodio, R., and Rath, S., 2016, Design of a new molecularly imprinted polymer selective for hydrochlorothiazide based on theoretical predictions using Gibbs free energy, J. Braz. Chem. Soc., 27 (12), 2300–2311.

[17] Triadhi, U., Zulfikar, M.A., Setiyanto, H., and Amran, M.B., 2018, Effects of (monomer - crosslinker – initiator) composition during non imprinted polymers synthesis for catechin retention, J. Phys.: Conf. Ser., 1013, 012192.

[18] Tomasello, C., Leggieri, A., Cavalli, R., Di Terri, G., and D’Avolio, A., 2015, In vitro stability evaluation of different pharmaceutical products containing meropenem, Hosp. Pharm., 50 (4), 296–303.

[19] Marić, M., Zhang, C., and Gromadzki, D., 2017, Poly(methacrylic acid-ran-2-vinylpyridine) statistical copolymer and derived dual pH-temperature responsive block copolymers by nitroxide-mediated polymerization, Processes, 5 (1), 7.

[20] Roth, T., Fiedler, S., Mihai, S., and Parsch, H., 2016, Determination of meropenem levels in human serum by high‐performance liquid chromatography with ultraviolet detection, Biomed Chromatogr., 31 (5), e3880.

[21] Khan, S.A., Siddiqui, M.F., and Khan, T.A., 2020, Synthesis of poly(methacrylic acid)/montmorillonite hydrogel nanocomposite for efficient adsorption of amoxicillin and diclofenac from aqueous environment: Kinetic, isotherm, reusability, and thermodynamic investigations, ACS Omega, 5 (6), 2843–2855.

[22] Qiu, L., Jaria, G., Gil, M.V., Feng, J., Dai, Y., Esteves, V.I., Otero, M., and Calisto, V., 2020, Core−shell molecularly imprinted polymers on magnetic yeast for the removal of sulfamethoxazole from water, Polymers, 12 (6), 1385.

[23] Sadeghi, S., and Jahani, M., 2013, Selective solid-phase extraction using molecular imprinted polymer sorbent for the analysis of florfenicol in food samples, Food Chem., 141 (2), 1242–1251.

[24] Serunting, M.A., Rusnadi, R., Setyorini, D.A., and Ramadan, B.S., 2018, An effective cerium(III) ions removal method using sodium alginate-coated magnetite (Alg-Fe3O4) nanoparticles, J. Water Supply: Res. Technol. - AQUA, 67 (8), 754–765.

[25] Li, Y., Xiao, H., Pan, Y., and Wang, L., 2018, Novel composite adsorbent consisting of dissolved cellulose fiber/microfibrillated cellulose for dye removal from aqueous solution, ACS Sustainable Chem. Eng., 6 (5), 6994–7002.

[26] Ayawei, N., Ebelegi, A.N., and Wankasi, D., 2017, Modelling and interpretation of adsorption isotherms, J. Chem., 2017, 3039817.

[27] Carvalho, M.N., de Abreu, C.A.M., Benachour, M., Sales, D.C.S., Baraúna, O.S., and da Motta Sobrinho, M.A., 2012, Applying combined Langmuir–Freundlich model to the multi-component adsorption of BTEX and phenol on smectite clay, Adsorpt. Sci. Technol., 30 (8-9), 691–699.

[28] Cao, Y., Xu, W., Wu, X., Li, Y., Li, H., and Huang, W., 2013, Synthesis of a molecularly imprinted polymer on silica-gel surfaces for the selective adsorption of indole from fuel oil, Adsorpt. Sci. Technol., 31 (6), 489–502.

[29] Huang, W., Li, H., Xu, W., Zhou, W., Zhou, Z., and Yang, W., 2012, Selective adsorption of dibenzothiophene using magnetic molecularly imprinted polymers, Adsorpt. Sci. Technol., 30 (4), 331–343.

[30] Douša, M., and Hosmanová, R., 2004, Rapid determination of amoxicillin in premixes by HPLC, J. Pharm. Biomed. Anal., 37 (2), 373–377.


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