Preparation, Characterization, and  In Vitro  Hemocompatibility of Glutaraldehyde-Crosslinked Chitosan/Carboxymethylcellulose as Hemodialysis Membrane

Khabibi Khabibi; Dwi Siswanta; Mudasir Mudasir

doi:10.22146/ijc.61704

Preparation, Characterization, and In Vitro Hemocompatibility of Glutaraldehyde-Crosslinked Chitosan/Carboxymethylcellulose as Hemodialysis Membrane

https://doi.org/10.22146/ijc.61704

Khabibi Khabibi⁽¹⁾, Dwi Siswanta⁽²⁾, Mudasir Mudasir^(3*)

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia; Department of Chemistry, Faculty of Sciences and Mathematics, Diponegoro University, Jl. Prof. H. Soedarto, S.H., Tembalang, Semarang 50275, Indonesia
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
(3) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
(*) Corresponding Author

Abstract

This study aims to examine the manufacture, characterization, and in vitro hemocompatibility of glutaraldehyde-crosslinked chitosan/carboxymethyl cellulose (CS/CMC-GA) as a hemodialysis membrane. The CS/CMC-GA membrane was prepared using the phase inversion method with 1.5% CS and 0.1% CMC. The chitosan was crosslinked with glutaraldehyde in various monomers ratios, and the membranes formed were characterized by FTIR, SEM, and TGA. Furthermore, the hydrophilicity, swelling, porosity, mechanical strength, and dialysis performance of the membranes against urea and creatinine were systematically examined, and their in-vitro hemocompatibility tests were also conducted. The results showed that the CS/CMC-GA membranes have higher hydrophilicity, swelling, porosity, mechanical strength, and better dialysis performance against urea and creatinine than chitosan without modification. In addition, the hemocompatibility test indicated that the CS/CMC-GA membranes have lower values of protein adsorption, thrombocyte attachment, hemolysis ratio, and partial thromboplastin time (PTT) than that of pristine chitosan. Based on these results, the CC/CMC-GA membranes have better hemocompatibility and the potential to be used as hemodialysis membranes.

Keywords

chitosan; CMC; membrane; hemodialysis

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DOI: https://doi.org/10.22146/ijc.61704