Determination of Eugenol in Personal-Care Products by Dispersive Liquid-Liquid Microextraction Followed by Spectrophotometry Using  p -Amino- N,N -dimethylaniline as a Derivatizing Agent

Bahaa Malik Altahir; Omar Abdulazeez; Sarmad Bahjat Dikran; Keith Edward Taylor

doi:10.22146/ijc.56198

Determination of Eugenol in Personal-Care Products by Dispersive Liquid-Liquid Microextraction Followed by Spectrophotometry Using p-Amino-N,N-dimethylaniline as a Derivatizing Agent

https://doi.org/10.22146/ijc.56198

Bahaa Malik Altahir^(1*), Omar Abdulazeez⁽²⁾, Sarmad Bahjat Dikran⁽³⁾, Keith Edward Taylor⁽⁴⁾

(1) Department of Biology, College of Science, University of Baghdad, Baghdad 10071, Iraq
(2) Department of Biology, College of Science, University of Baghdad, Baghdad 10071, Iraq
(3) Department of Chemistry, College of Education for Pure Science – Ibn Al-Haitham, University of Baghdad, Baghdad 10071, Iraq
(4) Department of Chemistry and Biochemistry, University of Windsor, 401 Sunset Avenue, Windsor, Ontario N9B 3P4, Canada
(*) Corresponding Author

Abstract

Two simple methods for the determination of eugenol were developed. The first depends on the oxidative coupling of eugenol with p-amino-N,N-dimethylaniline (PADA) in the presence of K₃[Fe(CN)₆]. A linear regression calibration plot for eugenol was constructed at 600 nm, within a concentration range of 0.25-2.50 μg.mL^–1 and a correlation coefficient (r) value of 0.9988. The limits of detection (LOD) and quantitation (LOQ) were 0.086 and 0.284 μg.mL^–1, respectively. The second method is based on the dispersive liquid-liquid microextraction of the derivatized oxidative coupling product of eugenol with PADA. Under the optimized extraction procedure, the extracted colored product was determined spectrophotometrically at 618 nm. A linear plot within a concentration range of 0.05–1.65 μg.mL^–1 (r = 0.9997) was constructed. The LOD and LOQ were 0.053 and 0.177 μg.mL^–1, respectively. Both methods were tested for the analysis of eugenol in commercial personal-care products, and the results confirmed that the procedures are accurate, precise, and reproducible (RSD < 1%).

Keywords

dispersive liquid-liquid microextraction; derivatizing agent; eugenol; p-amino-N,N-dimethylaniline

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DOI: https://doi.org/10.22146/ijc.56198