Proteins Pre-concentration Using Glycidyl Methacrylate-co-stearyl Methacrylate-co-ethylene Glycol Dimethacrylate Monolith

Ahmed Ali Alkarimi(1*), Kevin Welham(2)

(1) Department of Chemistry, College of Science, University of Babylon, Hilla 51002, Iraq
(2) Department of Chemistry, Faculty of Science and Engineering, University of Hull, Cottingham Road, Hull HU6 7RX, United Kingdom
(*) Corresponding Author


Solid-phase extraction technique is considered as a vital tool in a pre-treatment of different samples. Therefore, it was used as a beneficial and alternative extraction method over the liquid–liquid extraction in diverse applications such as food, environmental, and biological analyses. Glycidyl methacrylate-co-stearyl methacrylate-co-ethylene glycol dimethacrylate (GMA-co-SMA-co-EDMA) monolithic sorbent was prepared as strong cationic-reversed phase sorbent for solid-phase extraction of different proteins. The monolithic sorbent was prepared inside two moulds such as glass microchip device and borosilicate. The morphological properties have been investigated using the Brunauer-Emmett-Teller (BET) model analyzer and scanning electron microscope (SEM), and the formation of the monolithic sorbent was examined using the FT-IR. Eight proteins (cytochrome C, insulin, myoglobin, lysozyme, β-lactoglobulin, trypsin, albumin chicken egg white, and apo-transferrin) with different molecular weight and isoelectric point were investigated for pre-concentration using the SCX/RP sorbent. It was found that the highest extraction recovery was obtained with three proteins cytochrome C, lysozyme, and myoglobin compared with the other proteins. These three proteins were chosen for pre-concentration using the glass microchip. Sorbent performance showed significant results in terms of stability and reproducibility.


stearyl methacrylate; solid phase sorbent; glycidyl methacrylate; extraction

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