Statistical Modelling of Ultrasonic-Aided Extraction of Elaeis guineensis Leaves for Better-Quality Yield and Total Phenolic Content

https://doi.org/10.22146/ijc.41603

Nissha Bharrathi Romes(1), Mariani Abdul Hamid(2), Siti Ernieyanti Hashim(3), Roswanira Abdul Wahab(4*)

(1) Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor, Malaysia
(2) Institute of Bioproduct Development, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Malaysia
(3) Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor, Malaysia
(4) Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor, Malaysia
(*) Corresponding Author

Abstract


The present study highlighted the statistical modeling of an ultrasonic-aided extraction (UAE) of Elaeis guineensis leaves extract for maximal extraction yield (EY) and total phenolic content (TPC). A Box-Behnken design investigated the effects of ethanol concentration (X1: 0−100%), extraction time (X2: 5−55 min), the solvent-to-solid ratio (X3: 15:1−35:1 mL/g) and sonification amplitude (X4: 20−100 %). Under optimized conditions, the highest EY of 14.38% was attained using 50% (v/v) ethanol:water ratio, 55 min, 35 mL/g solvent-to-solid ratio, 60% sonication amplitude, whereas maximum TPC was 209.70 mg gallic acid equivalent (GAE)/g [50% (v/v) ethanol:water ratio, 30 min, 25 mL/g solvent-to-solid ratio, 60% sonication amplitude]. Second-order polynomial models of EY and TPC showed the R2 value corresponding to 0.9303 and 0.9500, respectively, indicating their significance (p < 0.05) to predict the responses. HPLC chromatograms revealed gallic acid and catechin were present in the UAE extracts. UAE technique afforded better EY (14.38%) and TPC (209.70 mg GAE/g) than maceration (3.73%, 85.23 mg GAE/g) and Soxhlet (6.86%, 102.13 mg GAE/g) extractions, as based on scanning electron micrographs of untreated, UAE, macerated and Soxhlet treated samples. Cell walls of ultrasonic-treated E. guineensis leaves were visibly disrupted to facilitate the higher release of bioactive plant materials, thus justifying the higher EY and TPC. The application of ultrasound appeared to remarkably increase the extraction efficiency of E. guineensis leaves to extract as compared to the conventional methods.


Keywords


ultrasonic-aided extraction; Elaeis guineensis; extraction yield; total phenolic content; response surface methodology

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References

[1] Dal Prá, V., Soares, J.F., Monego, D.L., Vendruscolo, R.G., Freire, D.M.G., Alexandri, M., and da Rosa, M.B. 2016, Extraction of bioactive compounds from palm (Elaeis guineensis) pressed fiber using different compressed fluids, J. Supercrit. Fluids, 112, 51–56.

[2] Dal Prá, V., Lunelli, F.C., Vendruscolo, R.G., Martins, R., Wagner, R., Lazzaretti Jr, A.P., Freire, D.M.G., Alexandri, M., Koutinas, M., Mazutti, M.A., and da Rosa, M.B., 2017, Ultrasound-assisted extraction of bioactive compounds from palm pressed fiber with high antioxidant and photoprotective activities, Ultrason. Sonochem., 36, 362–366.

[3] Obahiagbon, F., 2012, A review: Aspects of the African oil palm (Elaeis guineesis Jacq.) and the implications of its bioactives in human health, Am. J. Biochem. Mol. Biol., 2 (3), 106–119.

[4] Sumathi, S., Chai, S., and Mohamed, A., 2008, Utilization of oil palm as a source of renewable energy in Malaysia, Renewable Sustainable Energy Rev., 12 (9), 2404–2421.

[5] Onoja, E., Chandren, S., Razak, F.I.A., and Wahab, R.A., 2018, Enzymatic synthesis of butyl butyrate by Candida rugosa lipase supported on magnetized-nanosilica from oil palm leaves: Process optimization, kinetic and thermodynamic study, J. Taiwan Inst. Chem. Eng., 91, 105–118.

[6] Onoja, E., Chandren, S., Razak, F.I.A., Mahat, N.A., and Wahab, R.A., 2018, Oil palm (Elaeis guineensis) biomass in Malaysia: The present and future prospects, Waste Biomass Valorization, 1–19.

[7] Tahir, N.I., Shaari, K., Abas, F., Parveez, G.K.A., Ishak, Z., and Ramli, U.S., 2012, Characterization of apigenin and luteolin derivatives from oil palm (Elaeis guineensis Jacq.) leaf using LC–ESI-MS/MS, J. Agric. Food Chem., 60 (45), 11201–11210.

[8] Mohamed, S., 2014, Oil palm leaf: A new functional food ingredient for health and disease prevention, Int. J. Food Process. Technol., 5 (2), 1000300.

[9] Elias, N., Chandren, S., Attan, N., Mahat, N.A., Razak, F.I.A., Jamalis, J., and Wahab, R.A., 2017, Structure and properties of oil palm-based nanocellulose reinforced chitosan nanocomposite for efficient synthesis of butyl butyrate, Carbohydr. Polym., 176, 281–292.

[10] Sundram, K., Sambanthamurthi, R., and Tan, Y.A., 2003, Palm fruit chemistry and nutrition, Asia Pac. J. Clin. Nutr., 12 (3), 355–362.

[11] Mba, O.I., Dumont, M.J., and Ngadi, M., 2015, Palm oil: Processing, characterization and utilization in the food industry–A review, Food Biosci., 10, 26–41.

[12] Ofori-Boateng, C., Lee, K.T., and Saad, B., 2014, A biorefinery concept for simultaneous recovery of cellulosic ethanol and phenolic compounds from oil palm fronds: Process optimization, Energy Convers. Manage., 81, 192–200.

[13] Yusof, N.Z., Abd Gani, S.S., Hasan, Z.A.A., and Idris, Z., 2018, Skin and eye irritation assessment of oil palm (Elaeis guineensis) leaf extract for tropical application, Int. J. Toxicol., 37 (4), 335–343.

[14] Jaffri, J.M., Mohamed, S., Ahmad, I.N., Mustapha, N.M., Manap, Y.A., and Rohimi, N., 2011, Effects of catechin-rich oil palm leaf extract on normal and hypertensive rats’ kidney and liver, Food Chem., 128 (2), 433–441.

[15] Yusof, N.Z., Abd Gani, S.S., Siddiqui, Y., Mohd Mokhtar, N.F., and Hasan, Z.A.A. 2016, Potential uses of oil palm (Elaeis guineensis) leaf extract in topical application, J. Oil Palm Res., 28 (4), 520–530.

[16] Sharmila, G., Nikitha, V., Ilaiyarasi, S., Dhivya, K., Rajasekar, V., Kumar, N.M., Muthukumaran, K., and Muthukumaran, C., 2016, Ultrasound assisted extraction of total phenolics from Cassia auriculata leaves and evaluation of its antioxidant activities, Ind. Crops Prod., 84, 13–21.

[17] Hammi, K.M., Jdey, A., Abdelly, C., Majdoub, H., and Ksouri, R., 2015, Optimization of ultrasound-assisted extraction of antioxidant compounds from Tunisian Zizyphus lotus fruits using response surface methodology, Food Chem., 184, 80–89.

[18] Neo, Y.P., Ariffin, A., Tan, C.P., and Tan, Y.A., 2010, Phenolic acid analysis and antioxidant activity assessment of oil palm (E. guineensis) fruit extracts, Food Chem., 122 (1), 353–359.

[19] Rao, P., and Rathod, V., 2017, “Phytochemicals: An Insight to Modern Extraction Technologies and Their Applications” in Ingredients Extraction by Physicochemical Methods in Food, Elsevier, 495–521.

[20] Wang, X., Wu, Y., Chen, G., Yue, W., Liang, Q., and Wu, Q., 2013, Optimisation of ultrasound assisted extraction of phenolic compounds from Sparganii rhizoma with response surface methodology, Ultrason. Sonochem., 20 (3), 846–854.

[21] Wu, Y., Wang, X., Xue, J., and Fan, E., 2017, Plant phenolics extraction from Flos chrysanthemi: Response surface methodology based optimization and the correlation between extracts and free radical scavenging activity, J. Food Sci., 82 (11), 2726–2733.

[22] Xie, Z., Sun, Y., Lam, S., Zhao, M., Liang, Z., Yu, X., Yang, D., and Xu, X., 2014, Extraction and isolation of flavonoid glycosides from Flos Sophorae Immaturus using ultrasonic‐assisted extraction followed by high‐speed countercurrent chromatography, J. Sep. Sci., 37 (8), 957-965.

[23] Amiri, S., Shakeri, A., Sohrabi, M.R., Khalajzadeh, S., and Ghasemi, E., 2019, Optimization of ultrasonic assisted extraction of fatty acids from Aesculus hippocastanum fruit by response surface methodology, Food Chem., 271, 762–766.

[24] Deng, G.F., Xu, D.P., Li, S., and Li, H.B., 2015, Optimization of ultrasound-assisted extraction of natural antioxidants from sugar apple (Annona squamosa L.) peel using response surface methodology, Molecules, 20 (11), 20448–20459.

[25] Zhu, C.P., Zhai, X.C., Li, L.Q., Wu, X.X., and Li, B., 2015, Response surface optimization of ultrasound-assisted polysaccharides extraction from pomegranate peel, Food Chem., 177, 139–146.

[26] Manan, F.M.A., Attan, N., Zakaria, Z., Keyon, A.S.A., and Wahab, R.A. 2018, Enzymatic esterification of eugenol and benzoic acid by a novel chitosan-chitin nanowhiskers supported Rhizomucor miehei lipase: Process optimization and kinetic assessments, Enzyme Microb. Technol., 108, 42–52.

[27] Mohamad, N., Huyop, F., Aboul-Enein, H.Y., Mahat, N.A., and Wahab, R.A., 2015, Response surface methodological approach for optimizing production of geranyl propionate catalysed by carbon nanotubes nanobioconjugates, Biotechnol. Biotechnol. Equip., 29 (4), 732–739.

[28] Ali, A., Lim, X.Y., Chong, C.H., Mah, S.H., and Chua, B.L, 2018, Optimization of ultrasound-assisted extraction of natural antioxidants from Piper betle using response surface methodology, LWT, 89, 681–688.

[29] Dróżdż, P., Šėžienė, V., and Pyrzynska, K., 2017, Phytochemical properties and antioxidant activities of extracts from wild blueberries and lingonberries, Plant Foods Hum. Nutr., 72 (4), 360–364.

[30] Josipović, A., Sudar, R., Sudarić, A., Jurković, V., Kočar, M.M., and Kulundžić, A.M., 2016, Total phenolic and total flavonoid content variability of soybean genotypes in eastern Croatia, Croat. J. Food. Sci. Technol., 8 (2), 60–65.

[31] Zhang, H., Birch, J., Xie, C., Yang, H., Dias, G., Kong, L., and Bekhit, A.E.D. 2018, Optimization of extraction parameters of antioxidant activity of extracts from New Zealand and Chinese Asparagus officinalis L root cultivars, Ind. Crops Prod., 119, 191–200.

[32] Xu, D.P., Zheng, J., Zhou, Y., Li, Y., Li, S., and Li, H.B., 2017, Ultrasound-assisted extraction of natural antioxidants from the flower of Limonium sinuatum: Optimization and comparison with conventional methods, Food Chem., 217, 552–559.

[33] Yang, B., Liu, X., and Gao, Y., 2009, Extraction optimization of bioactive compounds (crocin, geniposide and total phenolic compounds) from Gardenia (Gardenia jasminoides Ellis) fruits with response surface methodology, Innovative Food Sci. Emerg. Technol., 10 (4), 610–615.

[34] Marzuki, N.H.C., Hamid, M.A., and Wahab, R.A., 2018, Assessment of fatty acid composition and response surface optimization of ultrasonic-assisted extraction of phenolic compounds from Pouteria campechiana pulp, Malays. J. Fundam. Appl. Sci., 14 (2), 269–277.

[35] Chua, S.C., Tan, C.P., Mirhosseini, H., Lai, O.M., Long, K., and Baharin, B.S., 2009, Optimization of ultrasound extraction condition of phospholipids from palm-pressed fiber, J. Food Eng., 92 (4), 403–409.

[36] López, C.J., Caleja, C., Prieto, M., Barreiro, M.F., Barros, L., and Ferreira, I.C.F.R., 2018, Optimization and comparison of heat and ultrasound assisted extraction techniques to obtain anthocyanin compounds from Arbutus unedo L. fruits, Food Chem., 264, 81–91.

[37] Jibril, S., Basar, N., Sirat, H.M., Wahab, R.A., Mahat, N.A., Nahar, L., and Sarker, S.D., 2018, Application of Box–Behnken design for ultrasound‐assisted extraction and recycling preparative HPLC for isolation of anthraquinones from Cassia singueana, Phytochem. Anal., 30 (1), 101–109.

[38] Shalmashi, A., 2009, Ultrasound‐assisted extraction of oil from tea seeds, J. Food Lipids, 16 (4), 465–474.

[39] Sheng, Z., Wang, Y., Wan, P., and Li, Y., 2014, Ultrasound-assisted extraction of total flavonoids from leaves of Syringa oblata Lindl., Lat. Am. Appl. Res., 44 (2), 131–135.

[40] Şahin, S., and Şamlı, R., 2013, Optimization of olive leaf extract obtained by ultrasound-assisted extraction with response surface methodology, Ultrason. Sonochem., 20 (1), 595–602.

[41] Ghitescu, R.E., Volf, I., Carausu, C., Bühlmann, A.M., Gilca, I.A., and Popa, V.I., 2015, Optimization of ultrasound-assisted extraction of polyphenols from spruce wood bark, Ultrason. Sonochem., 22, 535–541.

[42] Tomšik, A., Pavlić, B., Vladić, J., Ramić, M., Brindza, J., and Vidović, S., 2016, Optimization of ultrasound-assisted extraction of bioactive compounds from wild garlic (Allium ursinum L.), Ultrason. Sonochem., 29, 502–511.

[43] Esclapez, M., García-Pérez, J., Mulet, A., and Cárcel, J., 2011, Ultrasound-assisted extraction of natural products, Food Eng. Rev., 3 (2), 108.

[44] Vajić, U.J., Grujić-Milanović, J., Živković, J., Šavikin, K., Gođevac, D., Miloradović, Z., Bugarski, B., and Mihailović-Stanojević, N., 2015, Optimization of extraction of stinging nettle leaf phenolic compounds using response surface methodology, Ind. Crops Prod., 74, 912–917.

[45] Li, T., Qu, X.Y., Zhang, Q.A., and Wang, Z.Z., 2012, Ultrasound-assisted extraction and profile characteristics of seed oil from Isatis indigotica Fort, Ind. Crops Prod., 35 (1), 98–104.

[46] Minjares-Fuentes, R., Femenia, A., Garau, M., Meza-Velázquez, J., Simal, S., and Rosselló, C., 2014, Ultrasound-assisted extraction of pectins from grape pomace using citric acid: A response surface methodology approach, Carbohydr. Polym., 106, 179–189.

[47] Ahmad, N., Hasan, Z.A.A., Muhamad, H., Bilal, S.H., Yusof, N.Z., and Idris, Z., 2018, Determination of total phenol, flavonoid, antioxidant activity of oil palm leaves extracts and their application in transparent soap, J. Oil Palm Res., 30 (2), 315–325.



DOI: https://doi.org/10.22146/ijc.41603

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