Application of Functionalized Multi-Walled Carbon Nanotubes for Growth Enhancement of Mustard Seed Germination

Agus Subagio(1*), Erma Prihastanti(2), Ngadiwiyana Ngadiwiyana(3)

(1) Department of Physics, Faculty of Science and Mathematics, Universitas Diponegoro, Jl. Prof. Sudarto, S.H. Tembalang, Semarang 50275, Indonesia
(2) Department of Biology, Faculty of Science and Mathematics, Universitas Diponegoro, Jl. Prof. Sudarto, S.H. Tembalang, Semarang 50275, Indonesia
(3) Department of Chemistry, Faculty of Science and Mathematics, Universitas Diponegoro, Jl. Prof. Sudarto, S.H. Tembalang, Semarang 50275, Indonesia
(*) Corresponding Author


Multi-walled carbon nanotubes (MWCNTs) are one of the nanomaterials that can be applied to agriculture. This work investigates the beneficial effects of MWCNT function on mustard plants. In this study, the material of MWCNTs is functionalized with nitric acid to attach the carboxylic group onto the tube wall. The functionalized MWCNTs were characterized by SEM, TEM, XRD, and FTIR. The MWCNT diameter produced ranges from 20 to 50 nm and the inner diameter is 5 to 10 nm at the pyrolysis temperature of 900 °C. It was found that crystallites of the MWCNTs have (002) and (100) directions. There is a weak peak in MWCNTs prior to the functionalization process due to the presence of metal carbide (Fe3C), which serves as an active catalyst. FTIR results clearly indicate the presence of hydroxyl and carboxylic groups. These functionalized MWCNTs were dispersed into distilled water with various concentrations at 25, 50 and 75 µg/mL. By utilizing an immersion time of 24 h, mustard (Brassica juncea) seeds were soaked in each functionalized and non-functionalized MWCNT solution. Functionalized MWCNT solution at a concentration of 50 µg/mL was found to affect the growth of mustard seeds more significantly.


multi-walled carbon nanotubes; functionalization; mustard; seed; germination

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[1] Veronica, N., Guru, T., Thatikunta, R., and Reddy, S.N., 2015, Role of nano fertilizers in agricultural farming, Int. J. Environ. Sci. Technol., 1 (1), 1–3.

[2] Kottegoda, N., Munaweera, I., Madusanka, N., and Karunaratne, V., 2011, A green slow-release fertilizer composition based on urea-modified hydroxyapatite nanoparticles encapsulated wood, Curr. Sci., 101 (1), 73–78.

[3] Manjunatha, S.B., Biradar, D.P., and Aladakatti, Y.R., 2016, Nanotechnology and its applications in agriculture: A review, J. Farm Sci., 29 (1), 1–13.

[4] Subagio, A., Prihastanti, E., Ngadiwiyana, Rowi, K., and Gufron, A., 2015, Fabrication of NanoChiSil for Application of Fertilizer, Proceeding of the 5th International Seminar on New Paradigm and Innovation on Natural Sciences and Its Application (5th ISNPINSA), 7-8 October 2015, Semarang, Indonesia, 113–116.

[5] Aguilar-Elguezabal, A., Antunez, W., Alonso, G., Espinosa, F., and Miki-Yoshida, M., 2006, Study of carbon nanotubes synthesis by spray pyrolysis and model of growth, Diamond Relat. Mater., 15 (9), 1329–1335.

[6] Mukesh, T., and Jha, A.K., 2017, A review on: carbon nanotubes are vital for plant growth, Am. J. Agric. For., 5 (5-1), 1–9.

[7] Tiwari, D.K., Dasgupta-Schubert, N., Cendejas, L.M.V., Villegas, J., Montoya, L.C., and García, S.E.B., 2014, Interfacing carbon nanotubes (CNT) with plants: Enhancement growth, water and ionic nutrient uptake in maize (Zea mays) and implications for nanoagriculture, Appl. Nanosci., 4 (5), 577–591.

[8] Rameshaiah, G.N., Pallavi, J., and Shabnam, S., 2015, Nano fertilizers and nano sensors – an attempt for developing smart agriculture, Int. J. Eng. Res. Gen. Sci., 3 (1), 314–320.

[9] Yatim, N.M., Shaaban, A., Dimin, M.F., and Yusof, F., 2015, Statistical evaluation of the production of urea fertilizer-multiwalled carbon nanotubes using Plackett Burman experimental design, Procedia Soc. Behav. Sci., 195, 315–323.

[10] Tonucci, M.C., Gurgel, L.V.A., and de Aquino, S.F., 2015, Activated carbons from agricultural byproducts (pine tree and coconut shell), coal, and carbon nanotubes as adsorbents for removal of sulfamethoxazole from spiked aqueous solutions: Kinetic and thermodynamic studies, Ind. Crops Prod., 74, 111–121.

[11] Park, S., and Ahn, Y.J., 2016, Multi-walled carbon nanotubes and silver nanoparticles differentially affect seed germination, chlorophyll content, and hydrogen peroxide accumulation in carrot (Daucus carota L.), Biocatal. Agric. Biotechnol., 8, 257–262.

[12] Shen, X., Li, S., Zhang, H., Chen, W., Yang, Y., Li, J., Tao, S., and Wang, X., 2018, Effect of multiwalled carbon nanotubes on uptake of pyrene by cucumber (Cucumis sativus L.): Mechanistic perspectives, NanoImpact, 10, 168–176.

[13] Srivastava, A., and Rao, D.P., 2014, Enhancement of seed germination and plant growth of wheat, maize, peanut and garlic using multiwalled carbon nanotubes, Eur. Chem. Bull., 3 (5), 502–504.

[14] Sharma, R., Sharma, A.K., and Sharma, V., 2015, Synthesis of carbon nanotubes by arc-discharge and chemical vapor deposition method with analysis of its morphology, dispersion and functionalization characteristics, Cogent Eng., 2 (1), 1094017.

[15] Kumar, M., and Ando, Y., 2010, Chemical vapor deposition of carbon nanotubes: A review on growth mechanism and mass production, J. Nanosci. Nanotechnol., 10, 3739–3758.

[16] Arora, N., and Sharma, N.N., 2014, Arc discharge synthesis of carbon nanotubes: Comprehensive review, Diamond and Relat. Mater., 50, 135–150.

[17] Chrzanowska, J., Hoffman, J., Małolepszy, A., Mazurkiewicz, M., Kowalewski, T.A., Szymanski, Z., and Stobinski, L., 2015, Synthesis of carbon nanotubes by the laser ablation method: Effect of laser wavelength, Phys. Status Solidi B, 252 (8), 1860–1867.

[18] Vilatela, J.J., Rabanal, M.E., Cervantes-Sodi, F., García-Ruiz, M., Jiménez-Rodríguez, J.A., Reiband, G., and Terrones, M., 2015, A spray pyrolysis method to grow carbon nanotubes on carbon fibres, steel and ceramic bricks, J. Nanosci. Nanotechnol., 15 (4), 2858–2864.

[19] Annu, A., Bhattacharya, B., Singh, P.K., Shukla, P.K., and Rhee, H.W., 2017, Carbon nanotube using spray pyrolysis: Recent scenario, J. Alloys Compd., 691, 970–982.

[20] Khodakovskaya, M.V., Dervishi, E., Mahmood, M., Xu, Y., Li, Z., Watanabe, F., and Biris, A.S., 2009, Carbon nanotubes are able to penetrate plant seed coat and dramatically affect seed germination and plant growth, ACS Nano, 3 (10), 3221–3227.

[21] Tripathi, S., Sonkar, S.K., and Sarkar, S., 2011, Growth stimulation of gram (Cicer arietinum) plant by water soluble carbon nanotubes, Nanoscale, 3 (3), 1176–1181.

[22] Ahmed, D.S., Haider, A.J., and Mohammad, M.R., 2013, Comparison of Functionalization of multiwalled carbon nanotubes treated by oil olive and nitric acid and their characterization, Energy Procedia, 36, 1111–1118.

[23] Takada, T., Santida, D., and Abe, S., 2010, Suitable conditions for sidewall carboxylation of multiwalled carbon nanotubes, Nano Biomed., 2 (2), 147–152.

[24] Jun, L.Y., Mubarak, N.M., Yon, L.S., Bing, C.H., Khalid, M., and Abdullah, C, 2018, Comparative study of acid functionalization of carbon nanotube via ultrasonic and reflux mechanism, J. Environ. Chem. Eng., 6 (5), 5889–5896.

[25] Khodakovskaya, M.V., de Silva, K., Biris, A.S., Dervishi, E., and Villagarcia, H., 2012, Carbon nanotubes induce growth enhancement of tobacco cells, ACS Nano, 6 (3), 2128–2135.

[26] Begum, P., Ikhtiari, R., and Fugetsu, B., Matsuoka, M., Akasaka, T., and Watari, F., 2012, Phytotoxicity of multi-walled carbon nanotubes assessed by selected plant species in the seedling stage, Appl. Surf. Sci., 262, 120–124.

[27] Jeon, I.Y., Chang, D.W., Kumar, N.A., and Baek, J.B., 2011, “Functionalization of Carbon Nanotubes” in Carbon Nanotubes-Polymer Nanocomposites, Eds., Yellampalli, S., IntechOpen, London.

[28] Hamilton, R.F., Wu, Z., Mitra, S., and Holian, A., 2018, The effects of varying degree of MWCNT carboxylation on bioactivity in various in vivo and in vitro exposure models, Int. J. Mol. Sci., 19 (2), 354.

[29] Edward, E.R., Antunes, E.F., Botelho, E.C., Baldan, M.R., and Corat, E.J., 2011, Evaluation of residual iron in carbon nanotubes purified by acid treatments, Appl. Surf. Sci., 258 (2), 641–648.

[30] Stancu, M., Ruxanda, G., Ciuparu, D., and Dinescu, A., 2011, Purification of multiwall carbon nanotubes obtained by AC arc discharge method, Optoelectron. Adv. Mater. Rapid Commun., 5 (8), 846–850.

[31] Pisal, S.H., Harale, N.S., Bhat, T.S., Deshmukh, H.P., and Patil, P.S., 2014, Functionalized multi-walled carbon nanotubes for nitrogen sensor, IOSR J. Appl. Chem., 7 (11), 49–52.

[32] Das, R., Abd Hamid, S.B., Ali, M.E., Ramakrishna, S., and Yongzhi, W, 2015, Carbon nanotubes characterization by X-ray powder diffraction – A review, Curr. Nanosci., 11 (1), 23–35.

[33] Lambin, P., Loiseau, A., Culot, C., and Biro, L.P., 2002, Structure of carbon nanotubes probed by local and global probes, Carbon, 40 (10), 1635–1648.

[34] Wulandari, S.A., Arifin, Widiyandari, H., and Subagio, A., 2018, Synthesis and characterization carboxyl functionalized multi-walled carbon nanotubes (MWCNT-COOH) and NH2 functionalized multi-walled carbon nanotubes (MWCNT-NH2), J. Phys. Conf. Ser., 1025, 012005.

[35] Cui, H., Yan, X., Monasterio, M., and Xing, F., 2017, Effects of various surfactants on the dispersion of MWCNTs–OH in aqueous solution, Nanomaterials, 7 (9), 262.

[36] Lee, J., Kim, M., Hong, C.K., and Shim, S.E., 2007, Measurement of the dispersion stability of pristine and surface-modified multiwalled carbon nanotubes in various nonpolar and polar solvents, Meas. Sci. Technol., 18 (12), 3707–3712.

[37] Chen, G., Qiu, J., Liu, Y., Jiang, R., Cai, S., Liu, Y., Zhu, F., Zeng, F., Luan, T., and Ouyang, G., 2015, Carbon nanotubes act as contaminant carriers and translocate within plants, Sci. Rep., 5, 15682.


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