Semirefined Carrageenan (SRC) Film Incorporated with α-Tocopherol and Persicaria minor for Meat Patties Application

Khadijah Husna Abd Hamid(1), Wan Amnin Wan Yahaya(2), Neenasha Bebe Mohd Nor(3), Alyaa Syahierra Ghazali(4), Siti Kholijah Abdul Mudalip(5), Norashikin Mat Zain(6), Maria Pilar Almajano(7), Nurul Aini Mohd Azman(8*)

(1) Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak 26300, Gambang, Pahang, Malaysia
(2) Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak 26300, Gambang, Pahang, Malaysia
(3) Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak 26300, Gambang, Pahang, Malaysia
(4) Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak 26300, Gambang, Pahang, Malaysia
(5) Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak 26300, Gambang, Pahang, Malaysia
(6) Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak 26300, Gambang, Pahang, Malaysia
(7) Department of Chemical Engineering, Technical University of Catalonia, Avigunda Diagonal 647, Barcelona 08028, Spain
(8) Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak 26300, Gambang, Pahang, Malaysia
(*) Corresponding Author


Semirefined carrageenan (SRC) plasticized with glycerol (G) and incorporated with antioxidants of 0.4% (v/v) of α-tocopherol and Persicaria minor (PM) extract was successfully developed. The objective of this study is to analyze the antioxidant effect of active packaging films from semirefined carrageenan incorporated with α-tocopherol and Persicaria minor on meat patties. Total phenolic content and antioxidant activity of α-tocopherol and PM extract were measured. The effects of α-tocopherol and PM extract incorporated with SRC-based films on meat patties were evaluated using thiobarbituric acid reactive substance (TBARS) assay, metmyoglobin assay, and pH value for 14 days of storage. The films with 0.4% (v/v) of α-tocopherol and PM extract exhibited a lower lipid oxidation in meat patties compared with that of control (SRC film only, p < 0.05). Also, a brown color development of the meat patties of less than 50% was observed at the end of the 14-day storage. Meanwhile, the pH values for all samples decreased throughout the storage period with the SRC+G+α-tocopherol film showed the highest pH value. Hence, the formulation of SRC film with α-tocopherol or PM extract could be used as an alternative packaging for extending the shelf life of food product with high fat content.


active packaging film; meat patties; α-tocopherol; Persicaria minor extract; lipid oxidation

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[1] Farhan, A., and Hani, N.M., 2017, Characterization of edible packaging films based on semi-refined kappa-carrageenan plasticized with glycerol and sorbitol, Food Hydrocolloids, 64, 48–58.

[2] Vieira, M.G.A., da Silva, M.A., dos Santos, L.O., and Beppu, M.M., 2011, Natural-based plasticizers and biopolymer films: A review, Eur. Polym. J., 47 (3), 254–263.

[3] Peelman, N., Ragaert, P., De Meulenaer, B., Adons, D., Peeters, R., Cardon, L., Van Impe, F., and Devlieghere, F., 2013, Application of bioplastics for food packaging, Trends Food Sci. Technol., 32 (2), 128–141.

[4] Campos, C.A., Gerschenson, L.N., and Flores, S.K., 2011, Development of edible films and coatings with antimicrobial activity, Food Bioprocess Technol., 4 (6), 849–875.

[5] Talón, E., Trifkovic, K.T., Nedovic, V.A., Bugarski, B.M., Vargas, M., Chiralt, A., and González-Martínez, C., 2017, Antioxidant edible films based on chitosan and starch-containing polyphenols from thyme extracts, Carbohydr. Polym., 157, 1153–1161.

[6] Shojaee-Aliabadi, S., Hosseini, H., Mohammadifar, M.A., Mohammadi, A., Ghasemlou, M., Ojagh, S.M., Hosseini, S.M., and Khaksar, R., 2013, Characterization of antioxidant-antimicrobial κ-carrageenan films containing Satureja hortensis essential oil, Int. J. Biol. Macromol., 52, 116–124.

[7] Rekemin, N.F.N., and Hanani, Z.A.N., 2017, Physicochemical characterization of kappa-carrageenan (Euchema cottoni) based films incorporated with various plant oils, Carbohydr. Polym., 157, 1479–1487.

[8] Koontz, J.L., 2016, “Packaging Technologies to Control Lipid Oxidation” in Oxidative Stability and Shelf Life of Foods Containing Oils and Fats, Eds., Hu, M., and Jacobsen, C., Elsevier Inc., 479–517.

[9] Serrano-León, J.S., Bergamaschi, K.B., Yoshida, C.M.P., Saldaña, E., Selani, M.M., Rios-Mera, J.D., Alencar, S.M., and Contreras-Castillo, C.J., 2018, Chitosan active films containing agro-industrial residue extracts for shelf life extension of chicken restructured product, Food Res. Int., 108, 93–100.

[10] Trifković, K.T., Milašinović, N.Z., Djordjević, V.B., Krušić, M.T.K., Knežević-Jugović, Z.D., Nedović, V.A., and Bugarski, B.M., 2014, Chitosan microbeads for encapsulation of thyme (Thymus serpyllum L.) polyphenols, Carbohydr. Polym., 111, 901–907.

[11] López, D., Márquez, A., Gutiérrez-Cutiño, M., Venegas-Yazigi, D., Bustos, R., and Matiacevich, S., 2017, Edible film with antioxidant capacity based on salmon gelatin and boldine, LWT Food Sci. Technol., 77, 160–169.

[12] Azman, N.A.M., Gallego, M.G., Segovia, F., Abdullah, S., Shaarani. S.M., and Almajano, M.P., 2016, Study of the properties of bearberry leaf extract as a natural antioxidant in model foods, Antioxidants, 5 (2), 11.

[13] Vital, A.C.P., Guerrero, A., Monteschio, J.D.O., Valero, M.V., Carvalho, C.B., De Abreu Filho, B.A., Madrona, G.S., and Do Prado, I.N., 2016, Effect of edible and active coating (with rosemary and oregano essential oils) on beef characteristics and consumer acceptability, PLoS ONE, 11 (8), 0160535.

[14] Liu, F., Xu, Q., Dai, R., and Ni, Y., 2015, Effects of natural antioxidants on colour stability, lipid oxidation and metmyoglobin reducing activity in raw beef patties, Acta Sci. Pol. Technol. Aliment., 14 (1), 37–44.

[15] Azzi, A., 2017, Many tocopherols, one vitamin E, Mol. Aspects Med., 61, 92–103.

[16] Peh, H.Y., Tan, W.S.D., Liao, W., and Wong, W.S.F., 2015, Vitamin E therapy beyond cancer: Tocopherol versus tocotrienol, Pharmacol. Ther., 162, 152–169.

[17] Noronha, C.M., de Carvalho, S.M., Lino, R.C., and Barreto, P.L.M., 2014, Characterization of antioxidant methylcellulose film incorporated with α-tocopherol nanocapsules, Food Chem., 159, 529–535.

[18] Martins, J.T., Cerqueira, M.A., and Vicente, A.A., 2012, Influence of α-tocopherol on physicochemical properties of chitosan-based films, Food Hydrocolloids, 27 (1), 220–227.

[19] Azman, N.A.M., Skowyra, M., Muhammad, K., Gallego, M.G., and Pablos, M.P.A., 2017, Evaluation of the antioxidant activity of Betula pendula leaves extract and its effects on model foods, Pharm. Biol., 55 (1), 912–919.

[20] Gallego, M.G., Gordon, M.H., Segovia, F., and Pablos, M.P.A., 2016, Gelatine-based antioxidant packaging containing Caesalpinia decapetala and Tara as a coating for ground beef patties, Antioxidants, 5 (2), 10.

[21] Samad, A.F.A., Nazaruddin, N., Sajad, M., Jani, J., Murad, A.M.A., Zainal, Z., and Ismail, I., 2017, Small RNA sequencing for secondary metabolite analysis in Persicaria minor, Genomics Data, 13, 3–4.

[22] Sulaiman, S.F., Sajak, A.A.B., Ooi, K.L., Supriatno, and Seow, E.M., 2011, Effect of solvents in extracting polyphenols and antioxidants of selected raw vegetables, J. Food Compost. Anal., 24 (4-5), 506–515.

[23] Mackeen, M.M., Ali, A.M., El-Sharkawy, S.H., Manap, M.Y., Salleh, K.M., Lajis, N.S., and Kawazu, K., 1997, Antimicrobial and cytotoxic properties of some Malaysian traditional vegetables (ulam), Int. J. Pharmacogn., 35 (3), 174–178.

[24] Mustapha, S., Chandar, H., Abidin, Z.Z., Saghravani, R., and Harun, M.Y., 2011, Production of semi-refined carrageenan from Eucheuma cottonii, J. Sci. Ind. Res., 70 (10), 865–870.

[25] Azman, N.A.M., Segovia, F., Martínez-Farré, X., Gil, E., and Almajano, M.P., 2014, Screening of antioxidant activity of Gentian lutea root and its application in oil-in-water emulsions, Antioxidants, 3 (2), 455–471.

[26] Azman, N.A.M., Husni, S., Almajano, M.P., and Gallego, M.G., 2013, Solvent effect on antioxidant activity and total phenolic content of Betula alba and Convolvulus arvensis, World Acad. Sci. Eng. Technol., 7 (5), 351–356.

[27] Gallego, M.G., Gordon, M.H., Segovia, F.J., Skowyra, M., and Almajano, M.P., 2013, Antioxidant properties of three aromatic herbs (rosemary, thyme and lavender) in oil-in-water emulsions, J. Am. Oil Chem. Soc., 90 (10), 1559–1568.

[28] Azman, N.A.M., Gallego, M.G., Julià, L., Fajari, L., and Almajano, M.P., 2015, The effect of Convolvulus arvensis dried extract as a potential antioxidant in food models, Antioxidants, 4 (1), 170–184.

[29] Xu, Z., Tang, M., Li, Y., Liu, F., Li, X., and Dai, R., 2010, Antioxidant properties of Du-zhong (Eucommia ulmoides Oliv.) extracts and their effects on color stability and lipid oxidation of raw pork patties, J. Agric. Food Chem., 58 (12), 7289–7296.

[30] Jin, S.K., Ha, S.R., and Choi, J.S., 2015, Effect of Caesalpinia sappan L. extract on physico-chemical properties of emulsion-type pork sausage during cold storage, Meat Sci., 110, 245–252.

[31] Distantina, S., Rochmadi, Fahrurrozi, M., and Wiratni, 2013, Preparation and characterization of glutaraldehyde-crosslinked kappa carrageenan hydrogel, Eng. J., 17 (3), 57–66.

[32] Rhim, J.W., and Wang, L.F., 2014, Preparation and characterization of carrageenan-based nanocomposite films reinforced with clay mineral and silver nanoparticles, Appl. Clay Sci., 97-98, 174–181.

[33] Oun, A.A., and Rhim, J.W., 2017, Carrageenan-based hydrogels and films: Effect of ZnO and CuO nanoparticles on the physical, mechanical, and antimicrobial properties, Food Hydrocolloids, 67, 45–53.

[34] Zainuddin, N.K., and Samsudin, A.S., 2018, Investigation on the effect of NH4Br at transport properties in κ–carrageenan based biopolymer electrolytes via structural and electrical analysis, Mater. Today Commun., 14, 199–209.

[35] Man, Y.B.C., Ammawath, W., and Mirghani, M.E.S., 2005, Determining α-tocopherol in refined bleached and deodorized palm olein by Fourier transform infrared spectroscopy, Food Chem., 90 (1-2), 323–327.

[36] Fernández-Agulló, A., Pereira, E., Freire, M.S., Valentão, P., Andrade, P.B., González-Álvarez, J., and Pereira, J.A., 2013, Influence of solvent on the antioxidant and antimicrobial properties of walnut (Juglans regia L.) green husk extracts, Ind. Crops Prod., 42, 126–132.

[37] Murad, M., Abdullah, A., and Mustapha, W.A.W., 2011, Total phenolic content and antioxidant activity of kesum (Polygonum minus), ginger (Zingiber officinale) and turmeric (Curcuma longa) extract, Int. Food Res. J., 18, 529–534.

[38] Qader, S.W., Abdulla, M.A., Chua, L.S., Najim, N., Zain, M.M., and Hamdan, S., 2011, Antioxidant, total phenolic content and cytotoxicity evaluation of selected Malaysian plants, Molecules, 16 (4), 3433–3443.

[39] Gupta, D.S., and Suh, N., 2016, Tocopherols in cancer: An update, Mol. Nutr. Food Res., 60 (6), 1354–1363.

[40] Benzie, I.F.F., and Strain, J.J., 1999, [2] Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration, Methods Enzymol., 299, 15–27.

[41] Müller, L., Theile, K., and Böhm, V., 2010, In vitro antioxidant activity of tocopherols and tocotrienols and comparison of vitamin E concentration and lipophilic antioxidant capacity in human plasma, Mol. Nutr. Food Res., 54 (5), 731–742.

[42] Lee, H.E., Kim, D.H., Park, S.J., Kim, J.M., Lee, Y.W., Jung, J.M., Lee, C.H., Hong, J.G., Liu, X., Cai, M., Park, K.J., Jang, D.S., and Ryu, J.H., 2012, Neuroprotective effect of sinapic acid in a mouse model of amyloid β1–42 protein-induced Alzheimer’s disease, Pharmacol. Biochem. Behav., 103 (2), 260–266.

[43] Krinsky, N.I., 2001, Carotenoids as antioxidants, Nutrition, 17 (10), 815–817.

[44] Wrona, M., Nerín, C., Alfonso, M.J., and Caballero, M.Á., 2017, Antioxidant packaging with encapsulated green tea for fresh minced meat, Innovative Food Sci. Emerg. Technol., 41, 307–313.

[45] Lorenzo, J.M., Batlle, R., and Gómez, M., 2014, Extension of the shelf-life of foal meat with two antioxidant active packaging systems, LWT Food Sci. Technol., 59 (1), 181–188.

[46] Skowyra, M., Falguera, V., Azman, N.A.M., Segovia, F., and Almajano, M.P., 2014, The effect of Perilla frutescens extract on the oxidative stability of model food emulsions, Antioxidants, 3 (1), 38–54.

[47] Kılıç, B., Şimşek, A., Claus, J.R., Karaca, E., and Bilecen, D., 2018, Improving lipid oxidation inhibition in cooked beef hamburger patties during refrigerated storage with encapsulated polyphosphate incorporation, LWT Food Sci. Technol., 92, 290–296.

[48] Jones, R.J., 2004, Observations on the succession dynamics of lactic acid bacteria populations in chill-stored vacuum-packaged beef, Int. J. Food Microbiol., 90 (3), 273–282.


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