Preparation of Poly-(GMA-EDA-β-CD-co-TMPTMA) Monolith as High Performance Liquid Chromatography Chiral Stationary Phase Column

Stevin Carolius Angga; Dias Septiana; Suci Amalia; Warsito Warsito; Elvina Dhiaul Iftitah; Akhmad Sabarudin

doi:10.22146/ijc.38556

Preparation of Poly-(GMA-EDA-β-CD-co-TMPTMA) Monolith as High Performance Liquid Chromatography Chiral Stationary Phase Column

https://doi.org/10.22146/ijc.38556

Stevin Carolius Angga^(1*), Dias Septiana⁽²⁾, Suci Amalia⁽³⁾, Warsito Warsito⁽⁴⁾, Elvina Dhiaul Iftitah⁽⁵⁾, Akhmad Sabarudin⁽⁶⁾

(1) Department of Chemistry, Brawijaya University, Jl. Veteran, Malang 65145, Indonesia
(2) Department of Chemistry, Brawijaya University, Jl. Veteran, Malang 65145, Indonesia
(3) Department of Chemistry, Brawijaya University, Jl. Veteran, Malang 65145, Indonesia
(4) Department of Chemistry, Brawijaya University, Jl. Veteran, Malang 65145, Indonesia
(5) Department of Chemistry, Brawijaya University, Jl. Veteran, Malang 65145, Indonesia
(6) Department of Chemistry, Brawijaya University, Jl. Veteran, Malang 65145, Indonesia
(*) Corresponding Author

Abstract

An enantiomer molecule consisted of the chiral atom has different structure conformations, which exhibit different activities as well. Yet, its separation considerably difficult since ordinary separation could not separate both molecules. One of the popular enantioseparations which are often used was using organic polymer monolithic column modified by ethylenediamine-β-cyclodextrin (EDA-β-CD) as the enantioseparations site. The aim of this research was to produce chiral stationary phase column for enantioseparations of (±)-citronellal. It was conducted by preparing monolithic column using monomer glycidyl methacrylate (GMA), trimethylolpropane trimethacrylate (TMPTMA) as crosslinker, 1-propanol, 1,4-butanediol, and water as pore-forming agents (porogens) in the presence of α,α'-azoisobutyronitrile (AIBN) as radical initiator inside polyetheretherketone (PEEK) tubing. It was then modified with EDA-β-CD synthesized from β-CD. Finally, it was installed as a high-performance liquid chromatography column. The result shows the produced chiral stationary phase column could separate (±)-citronellal at a retention time of 44.76 and 45.71 min.

Keywords

enantioseparations; cyclodextrin; organic polymer monolith; methacrylate; high-performance liquid chromatography

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DOI: https://doi.org/10.22146/ijc.38556