Synthesis of Graphite Porous Electrode Based on Coconut Shell as a Potential Cathode in Bioelectrosyntesis Cell

Aris Mukimin(1*), Rustiana Yuliasni(2), Nur Zen(3), Kukuh Wicaksono(4), Januar Arif Fatkhurahman(5), Hanny Vistanty(6), Rizal Awaludin Malik(7)

(1) Center of Industrial Prevention Technology, Jl. Ki Mangunsarkoro No. 6, PO Box. 829, Semarang 50136, Indonesia
(2) Center of Industrial Prevention Technology, Jl. Ki Mangunsarkoro No. 6, PO Box. 829, Semarang 50136, Indonesia
(3) Center of Industrial Prevention Technology, Jl. Ki Mangunsarkoro No. 6, PO Box. 829, Semarang 50136, Indonesia
(4) Center of Industrial Prevention Technology, Jl. Ki Mangunsarkoro No. 6, PO Box. 829, Semarang 50136, Indonesia
(5) Center of Industrial Prevention Technology, Jl. Ki Mangunsarkoro No. 6, PO Box. 829, Semarang 50136, Indonesia
(6) Center of Industrial Prevention Technology, Jl. Ki Mangunsarkoro No. 6, PO Box. 829, Semarang 50136, Indonesia
(7) Center of Industrial Prevention Technology, Jl. Ki Mangunsarkoro No. 6, PO Box. 829, Semarang 50136, Indonesia
(*) Corresponding Author


Electrodes, as well as microorganisms, are key materials for the development of bioelectrosynthesis cell reactor. Materials used as electrodes should be inert, crystalline in structure with high surface area and porous morphology, enhancing their electroactive and adsorptive properties. Carbon material derived from coconut shell was modified by simultaneous sintering-activation methods, FeCl3 and ZnCl2 were supplemented at temperature 900 °C at the non-atmospheric condition. The modified carbon was then molded with polyvinyl alcohol as a binder and the temperature was maintained at 80 °C and 10 ton of pressure. Molded carbon was then installed in bioelectrosynthesis cell with a working volume of 200 mL, as a cathode. XRD, BET, and SEM measurements showed the transformation of carbon surface from amorphous into the crystalline, increased surface area (11 times higher) and higher porosity (up to 500 nm). This cathode modification was able to increase current density up to 4 times and reduce CO2 into butyrate, 250 mg/L, in bioelectrosynthesis cell.


coconut shell carbon; bioelectrosynthesis; sintering-activation; carbon dioxide; butyrate

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[1] Lovley, D.R., and Nevin, K.P., 2013, Electrobiocommodities: Powering microbial production of fuels and commodity chemicals from carbon dioxide with electricity, Curr. Opin. Biotechnol., 24 (3), 385–390

[2] Shin, H.J., Jung, K.A., Nam, C.W., and Park, J.M., 2017, A genetic approach for microbial electrosynthesis system as biocommodities production platform, Bioresour. Technol., 245 (Part B), 1421–1429.

[3] Schuchmann, K., and Müller, V., 2014, Autotrophy at the thermodynamic limit of life: a model for energy conservation in acetogenic bacteria, Nat. Rev. Microbiol., 12 (12), 809–821.

[4] Nevin, K.P., Woodard, T.L., Franks, A.E., Summers, Z.M., and Lovley, D.R., 2010, Microbial electrosynthesis : Feeding microbes electricity to convert carbon dioxide and water to multicarbon extracellular organic compounds, MBio, 1 (2), 00103-10.

[5] Jiang, Y., Su, M., Zhang, Y., Zhan, G., Tao, Y., and Li, D., 2013, Bioelectrochemical systems for simultaneously production of methane and acetate from carbon dioxide at relatively high rate, Int. J. Hydrogen Energy, 38 (8), 3407–3502.

[6] Steinbusch, K.J.J., Hamelers, H.V.M., Schaap, J.D., Kampman, C., and Buisman, C.J.N., 2010, Bioelectrochemical ethanol production through mediated acetate reduction by mixed cultures, Environ. Sci. Technol., 44 (1), 513–517.

[7] Sakai, S., Nakashimada, Y., Yoshimoto, H., Watanabe, S., Okada, H., and Nishio, N., 2004, Ethanol production from H2 and CO2 by a newly isolated thermophilic bacterium, Moorella sp. HUC22-1, Biotechnol. Lett., 26 (20), 1607–1612.

[8] Van Eerten-Jansen, M.C.A.A., Ter Heijne, A., Grootscholten, T.M.I., Steinbusch, K.J.J., Sleutels, T.H.J.A., Hamelers, H.V.M., and Buisman, C.J.N., 2013, Bioelectrochemical production of caproate and caprylate from acetate by mixed cultures, ACS Sustainable Chem. Eng., 1 (5), 513–518

[9] Aryal, N., 2017, Microbial Electrosynthesis for Acetate Production from Carbon Dioxide : Innovative Biocatalysts Leading to Enhanced Performance, Dissertation, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Denmark.

[10] Liu, Y., Hu, Z., Xu, K., Zheng, X., and Gao, Q., 2008, Surface modification and performance of activated carbon electrode material, Acta Phys. Chim. Sin., 24 (7), 1143–1148.

[11] Guo, K., Prévoteau, A., Patil, S.A., and Rabaey, K., 2015, Engineering electrodes for microbial electrocatalysis, Curr. Opin. Biotechnol., 33, 149–156.

[12] Patil, S.A., Arends, J.B.A., Vanwonterghem, I., van Meerbergen, J., Guo, K., Tyson, G.W., and Rabaey, K., 2015, Selective enrichment establishes a stable performing community for microbial electrosynthesis of acetate from CO2, Environ. Sci. Technol., 49 (14), 8833–8843.

[13] Bajracharya, S., ter Heijne, A., Benetton, X.D., Vanbroekhoven, K., Buisman, C.J.N., Strik, D.P.B.T.B., and Pant, D., 2015, Carbon dioxide reduction by mixed and pure cultures in microbial electrosynthesis using an assembly of graphite felt and stainless steel as a cathode, Bioresour. Technol., 195, 14–24.

[14] Jourdin, L., Grieger, T., Monetti, J., Flexer, V., Freguia, S., Lu, K., Chen, J., Romano, M., Wallace, G.G., and Keller, J., 2015, High acetic acid production rate obtained by microbial electrosynthesis from carbon dioxide, Environ. Sci. Technol., 49 (22), 13566–13574.

[15] Zhang, T., Nie, H., Bain, T.S., Lu, H., Cui,M., Snoeyenbos-West, O.L., Franks, A.E., Nevin, K.P., Russell, T.P., and Lovley, D.R., 2013, Improved cathode materials for microbial electrosynthesis, Energy Environ. Sci., 6, 217–224.

[16] Marshall, C.W., Ross, D.E., Fichot, E.B., Norman, R.S., and May, H.D., 2012, Electrosynthesis of commodity chemicals by an autotrophic microbial community, Appl. Environ. Microbiol., 78 (23), 8412–8420.

[17] Angenent, L.T., and Rosenbaum, M.A., 2013, Microbial electrocatalysis to guide biofuel and biochemical bioprocessing, Biofuels, 4 (2), 131–134.

[18] Faraghiparapari, N., and Zengler, K., 2017, Production of organics from CO2 by microbial electrosynthesis (MES) at high temperature, J. Chem. Technol. Biotechnol., 92 (2), 375–381

[19] Dietz, S.D., and Nguyen, V., 2000, Monolithic Carbon Electrodes for Double Layer Capacitors, Proceedings for the 10th International Seminar on Double Layer Capacitors and Similar Energy Storage Devices, Deerfield Beach, Florida.

[20] Sun, L., Tian, C., Li, M., Meng, X., Wang, L., Wang, R., Yin, J., and Fu, H., 2013, From coconut shell to porous graphene-like nanosheets for high-power supercapacitors, J. Mater. Chem. A, 1 (21), 6462–6470.

[21] Rampe, M.J., Setiaji, B., and Trisunaryanti, W., 2011, Fabrication and characterization of carbon composite from coconut shell carbon, Indones. J. Chem., 11 (2), 124–130.

[22] Lim, H.N., Huang, N.M., Lim, S.S., Harrison, I., and Chia, C.H., 2011, Fabrication and characterization of graphene hydrogel via hydrothermal approach as a scaffold for preliminary study of cell growth., Int. J. Nanomed., 6, 1817–1823.

[23] Li, Z.Q., Lu, C.J., Xia, Z.P., Zhou, Y., and Luo, Z., 2007, X-ray diffraction patterns of graphite and turbostratic carbon, Carbon, 45 (8), 1686–1695.

[24] Mukimin, A., Vistanty, H., and Crisnaningtyas, F., 2015, Physico-chemical treatment enhancing electroactivity properties of coconut shell-based carbon electrode, Int. J. Appl. Chem., 11 (4), 553–565.

[25] Wang, L., Liu, W., He, Z., Guo, Z., Zhou, A., and Wang, A., 2017, Cathodic hydrogen recovery and methane conversion using Pt coating 3D nickel foam instead of Pt-carbon cloth in microbial electrolysis cells, Int. J. Hydrogen Energy, 42 (31), 19604–19610.

[26] Zhen, G., Zheng, S., Lu, X., Zhu, X., Mei, J., Kobayashi, T., Xu, K., Li, Y.Y., and Zhao, Y., 2018, A comprehensive comparison of five different carbon-based cathode materials in CO2 electromethanogenesis: Long-term performance, cell-electrode contact behaviors and extracellular electron transfer pathways, Bioresour. Technol., 266, 382–388.

[27] Batlle-Vilanova, P., Ganigué, R., Ramió-Pujol, S., Bañeras, L., Jiménez, G., Hidalgo, M., Balaguer, M.D., Colprim, J., and Puig, S., 2017, Microbial electrosynthesis of butyrate from carbon dioxide: Production and extraction, Bioelectrochemistry, 117, 57–64.

[28] Gössner, A.S., Picardal, F., Tanner, R.S., and Drake, H.L., 2008, Carbon metabolism of the moderately acid-tolerant acetogen Clostridium drakei isolated from peat, FEMS Microbiol. Lett., 287, 236–242. doi:10.1111/j.1574-6968.2008.01313.x.

[29] Xiang, Y., Liu, G., Zhang, R., Lu, Y., and Luo, H., 2017, Acetate production and electron utilization facilitated by sulfate-reducing bacteria in a microbial electrosynthesis system, Bioresour. Technol., 241, 821–829.

[30] Villano, M., Aulenta, F., Ciucci, C., Ferri, T., Giuliano, A., and Majone, M., 2010, Bioelectrochemical reduction of CO2 to CH4 via direct and indirect extracellular electron transfer by a hydrogenophilic methanogenic culture, Bioresour. Technol., 101 (9), 3085–3090.

[31] Sangeetha, T., Guo, Z., Liu, W., Cui, M., Yang, C., Wang, L., and Wang, A., 2016, Cathode material as an influencing factor on beer wastewater treatment and methane production in a novel integrated upflow microbial electrolysis cell (Upflow-MEC), Int. J. Hydrogen Energy, 41 (4), 2189–2196.

[32] Guo, Z., Thangavel, S., Wang, L., He, Z., Cai, W., Wang, A., and Liu, W., 2017, Efficient methane production from beer wastewater in a membraneless microbial electrolysis cell with a stacked cathode: The effect of the cathode/anode ratio on bioenergy recovery, Energy Fuels, 31 (1), 615–620.

[33] Liu, W., He, Z., Yang, C., Zhou, A., Guo, Z., Liang, B.. Varrone, C., and Wang, A.J., 2016, Microbial network for waste activated sludge cascade utilization in an integrated system of microbial electrolysis and anaerobic fermentation, Biotechnol. Biofuels, 9, 83.


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