Antibacterial Properties of Biofilm Schiff Base Derived from Dialdehyde Cellulose and Chitosan

https://doi.org/10.22146/ijc.34721

Agung Pratama(1), Firman Sebayang(2*), Rumondang Bulan Nasution(3)

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Sumatera Utara, Jl. Bioteknologi No.1, Kampus USU, Medan 20155, Indonesia
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Sumatera Utara, Jl. Bioteknologi No.1, Kampus USU, Medan 20155, Indonesia
(3) Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Sumatera Utara, Jl. Bioteknologi No.1, Kampus USU, Medan 20155, Indonesia
(*) Corresponding Author

Abstract


Cellulose and chitosan are natural polymers that have been used as biocomposite. The aim of this research is to obtain biofilms from chitosan and oxidized cellulose crosslinks. This research is divided into three steps, i.e., isolation of cellulose from oil palm trunk and oxidation of cellulose using NaIO4 (0.2; 0.4; 0.6; 0.8; 1.0 mg/mL) to obtain dialdehyde cellulose (DAC), crosslink of oxidized cellulose with chitosan (DD = 72.85%) to obtain biofilm of chitosan/DAC (CDAC), and characterization of biofilms. The crosslinked reaction was confirmed by FT-IR analysis that showed the spectrum of Schiff base C=N group at 1651 cm–1. Tensile strength increased gradually when the NaIO4 concentration used was 0.2–0.6 mg/mL, but after those concentrations, the tensile strength slightly decreased. The morphology analysis showed that CDAC had smoother morphology than DAC, which was shown rough and showed some particle indicated the presence of unreacted cellulose. CDAC biofilms that prepared with 1.0 mg/mL NaIO4 showed the greatest antibacterial activity.

Keywords


biofilm; cellulose; chitosan; dialdehyde cellulose, Schiff base

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References

[1] Ren, L., Yan, X., Zhou, J., Tong, J., and Su, X. , 2017, Influence of chitosan concentration on mechanical and barrier properties of cornstarch/chitosan films, Int. J. Biol. Macromol., 105 (Pt 3), 1636–1643.

[2] Mahieu, A., Terrié, C., and Youssef, B., 2015, Thermoplastic starch films and thermoplastic starch/polycaprolactone blends with oxygen-scavenging properties: influence of water content, Ind. Crops Prod., 72, 192–199.

[3] Aider, M., 2010, Chitosan application for active bio-based films production and potential in the food industry: Review, LWT Food Sci. Technol., 43 (6), 837–842.

[4] Dutta, P.K., Tripathi, S., Mehrotra, G.K., and Dutta, J., 2009, Perspectives for chitosan-based antimicrobial films in food applications, Food Chem., 114 (4), 1173–1182.

[5] Yang, W., Wang, Y., Li, X., and Yu, P., 2015, Purification and structural characterization of Chinese yam polysaccharide and its activities, Carbohydr. Polym., 117, 1021–1027.

[6] Tahmouzi, S., and Ghodsi, M., 2014, Optimum extraction of polysaccharides from motherwort leaf and its antioxidant and antimicrobial activities, Carbohydr. Polym., 112, 396–403.

[7] Sinha, S., Astani, A., Ghosh, T., Schnitzler, P., and Ray, B., 2010, Polysaccharides from Sargassum tenerrimum: Structural features, chemical modification, and anti-viral activity, Phytochemistry, 71 (2-3), 235–242.

[8] Ferreira, I.C.F.R., Heleno, S.A., Reis, F.S., Stojkovic, D., Queiroz, M.J.R.P., Vasconcelos, M.H., and Sokovic, M., 2015, Chemical features of Ganoderma polysaccharides with antioxidant, antitumor and antimicrobial activities, Phytochemistry, 114, 38–55.

[9] Liu, J.Y., Feng, C.P., Li, X., Chang, M.C., Meng, J.L., and Xu, L.J., 2016, The Immunomodulatory and antioxidative activity of Cordyceps militaris polysaccharides in mice, Int. J. Biol. Macromol., 86, 594–598.

[10] Bansal, M., Chauhan, G.S., Kaushik, A., and Sharma, A., 2016, Extraction and functionalization of bagasse cellulose nanofibres to Schiff-base based antimicrobial membranes, Int. J. Biol. Macromol., 91, 887–894.

[11] Jayakumar, R., Prabaharan, M., Nair, S.V., and Tamura, H., 2010, Novel chitin and chitosan nanofibres in biomedical applications, Biotechnol. Adv., 28 (1), 142–150.

[12] Nikolajski, M., Wotschadlo, J., Clement, J.H., and Heinze, T., 2012, Amino-functionalized cellulose nanoparticles: preparation, characterization, and interactions with living cells, Macromol. Biosci., 12 (7), 920–925.

[13] Zhang, L., Zhang, Q., Zheng, Y., He, Z., Guan, P., He, X., Hui, L., and Dai, Y., 2018, Study of Schiff base formation between dialdehyde cellulose and proteins, and its application for the deproteinization of crude polysaccharide extracts, Ind. Crops Prod., 112, 532–540.

[14] Shi, L., Ge, H.M., Tan, S.H., Li, H.Q., Song, Y.C., Zhu, H.L., and Tan, R.X., 2007, Synthesis and antimicrobial activities of Schiff bases derived from 5-chloro-salicylaldehyde, Eur. J. Med. Chem., 42 (4), 558–564.

[15] Strnad, S., Sauperl, O., and Fras-Zemljic, L., 2010, “Cellulose Fibres Functionalised by Chitosan: Characterization and Application” in Biopolymers, Eds., Elnashar, M., IntechOpen, 181–200.

[16] Ohwoavworhua, F.O., and Adelakun, T.A, 2005, Phosphoric acid-mediated depolymerization and decrystallization of α-cellulose obtained from corn cob: Preparation of low crystallinity cellulose and some physicochemical properties, Trop. J. Pharm. Res., 4 (2), 509–516.

[17] Liu, X., and Xu, Y., 2014, Preparation process and antimicrobial properties of cross-linking chitosan onto periodate-oxidized bamboo pulp fabric, Fibers Polym., 15 (9), 1887–1894.

[18] Noerati , 2008, Sintesis Kitosan Karboksilat Sebagai Zat Anti Bakteri dan Anti Kusut pada Material Selulosa, Dissertation, Department of Chemistry, ITB, Bandung.

[19] Janjić, S., Kostić, M., Škundrić, P., Lazić, B., and Praskalo, J., 2013, Antibacterial fibers based on cellulose and chitosan, Contemp. Mater., 2 (3), 207–218.

[20] Rangel-Vázquez, N.-A., Guilbert-Garcia, E., Salgado-Delgado, R., Rubio-Rosas, E., Hernandez, E.G., Vargas-Galarza, Z., and Crispin-Espino, I., 2010, Synthesis, and characterization of chitosan-coated dialdehyde cellulose with potential antimicrobial behavior, J. Mater. Sci. Eng., 4 (12), 62–67.

[21] Strnad, S., Sauper, O., and Jazbec, A., and Stana-Kleinschek, K., 2008, Influence of chemical modification on sorption and mechanical properties of cotton fibers treated with chitosan, Text. Res. J., 78 (5), 390–398.

[22] Hou, Q., Liu, W., Liu, Z., Duan, B., and Bai, L., 2008, Characteristics of antimicrobial fibers prepared with wood periodate oxycellulose, Carbohydr. Polym., 74 (2), 235–240.

[23] Pelczar, M.J., 1972, Food Microbiology, 5th ed., McGraw Hill Book Co. Inc., New York, 783



DOI: https://doi.org/10.22146/ijc.34721

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