Separation of Inorganic Anions and Phenolic Compounds Using Tetraethylene Oxide-Bonded Stationary Phases in Capillary Liquid Chromatography

Roza Linda(1*), Mohamad Rafi(2), Lee Wah Lim(3), Toyohide Takeuchi(4)

(1) Department of Chemistry Education, Faculty of Teacher Training and Education, University of Riau, Kampus Binawidya Km 12.5, Pekanbaru 28293, Indonesia
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Kampus IPB Dramaga, Bogor 16880, Indonesia
(3) Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University, Gifu, Japan
(4) Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University, Gifu, Japan
(*) Corresponding Author


Tetraethylene glycol monomethyl ether (TEGMM) and tetraethylene glycol (TEG) modified silica were synthesized and used in capillary liquid chromatography as stationary phases. The stationary phases were prepared by chemically bonding TEGMM or TEG on silica via reaction with 3-glycidyloxypropyltrimethoxysilane. The present stationary phases were successfully used for the separation of several inorganic anions and phenolic compounds. The TEG-modified silica stationary phase had a better selectivity and higher retention for five anions compared to TEGMM-modified silica stationary phase. The repeatability of retention time for the five anions was satisfactory on both stationary phases. By using 2 mM sodium chloride as eluent, the relative standard deviation values were in ranging from 0.68–3.21 and 2.00–2.16% for analytes in the TEG-and TEGMM-bonded stationary phase, respectively. It was found that the TEG-bonded stationary phase had hydrophilic properties due to the presence of the hydroxyl group at the end of the TEG chains.


capillary liquid chromatography; tetraethylene glycol monomethyl ether; tetraethylene glycol; inorganic anions; phenol compounds

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[1] Dean, T.H., and Jezorek, J.R., 2004, Demonstration of simultaneous anion-exchange and reversed-phase behavior on a strong anion-exchange column, J. Chromatogr. A, 1028 (2), 239—245.

[2] Small, H., Stevens, T.S., and Bauman, W.C., 1975, Novel ion exchange chromatographic method using conductimetric detection, Anal. Chem., 47 (1), 1801–1809.

[3] Qiu, H., Liang, X., Sun, M., and Jiang, S., 2011, Development of silica-based stationary phases for high-performance liquid chromatography, Anal. Bioanal. Chem., 399 (10), 3307–3322.

[4] Buszewski, B., Jaćkowska, M., Bocian, S., Kosobucki, P., and Gawdzik, B., 2011, Functionalized polymeric stationary phases for ion chromatography, J. Sep. Sci., 34 (6), 601–608.

[5] Liu, Y., Du, Q., Yang, B., Zhang, F., Chu, C., and Liang, X., 2012, Silica based click amino stationary phase for ion chromatography and hydrophilic interaction liquid chromatography, Analyst, 137 (7), 1624–1628.

[6] Takeuchi, T., and Ishii, D., 1981, High-performance micro packed flexible columns in liquid chromatography, J. Chromatogr. A, 213 (1), 25–32.

[7] Linda, R., Lim, L.W., and Takeuchi, T., 2013, Poly(ethylene oxide)-bonded stationary phase for separation of inorganic anions in capillary ion chromatography, J. Chromatogr. A, 1294, 117–121.

[8] Lim, L.W., Tokunaga, K., and Takeuchi. T., 2014, Development of chemically bonded crown ether stationary phases in capillary ion chromatography, Chromatography, 35 (2), 95–101.

[9] Aydoğan, C., 2015, A new anion-exchange/hydrophobic monolith as stationary phase for nano liquid chromatography of small organic molecules and inorganic anions, J. Chromatogr. A, 1392, 63–68.

[10] Kawase, I., Lim, L.W., and Takeuchi, T., 2017, Investigation of chromatographic performance of hyperbranched amine-modified stationary phases in ion chromatography, Chromatography, 38 (1), 9–14.

[11] Sun, M., Feng, J., Liu, S., Xiong, C., Liu, X., and Jiang, S., 2011, Dipyridine modified silica-A novel multi-interaction stationary phase for high performance liquid chromatography, J. Chromatogr. A, 1218 (24), 3743–3749.

[12] Lamb, J.D., and Smith, R.G., 1991, Application of macrocyclic ligands to ion chromatography, J. Chromatogr. A, 546, 73–88.

[13] Lamb, J.D., Smith, R.G., and Jagodzinski, J., 1993, Anion chromatography with crown ether-based stationary phase and an organic modifier in the eluent, J. Chromatogr. A, 640 (1-2), 33–40.

[14] Takeuchi, T., Oktavia, B., and Lim, L.W., 2009, Poly(ethylene oxide)-bonded stationary phase for capillary ion chromatography, Anal. Bioanal. Chem., 393 (4), 1267–1272.

[15] Takeuchi, T., and Lim, L.W., 2010, Multifunctional separation mechanism on poly(oxyethylene) stationary phases in capillary liquid chromatography, Anal. Sci., 26 (9), 937–941.

[16] Takeuchi, T., Tokunaga, K., and Lim, L.W., 2013, Separation of inorganic anions on a chemically bonded 18-crown-6 ether stationary phase in capillary ion chromatography, Anal. Sci., 29 (4), 423–427.

[17] Linda R., 2013, Development of stationary phase for separation of inorganic anions and organic compounds in capillary liquid chromatography, Dissertation, Material Engineering Division, Faculty of Engineering and Graduate School of Engineering, Gifu University, Japan.


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