Synthesis of Gold Nanoparticles Using p-Aminobenzoic Acid and p-Aminosalicylic Acid as Reducing Agent

Abdul Aji(1*), Eko Sri Kunarti(2), Sri Juari Santosa(3)

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
(3) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
(*) Corresponding Author


Synthesis of gold nanoparticles (AuNPs) by reduction of HAuCl4 with p-aminobenzoic acid and p-aminosalicylic acid as a reducing agent was investigated. This work was conducted in order to determine the optimum condition of AuNPs synthesis and examine the effect of the hydroxyl group in p-aminosalicylic acid towards the size, shape, and stability of the synthesized gold nanoparticles (AuNPs). The optimum condition of the gold nanoparticles synthesis was determined by UV/Vis spectrophotometer, the shape and size of gold nanoparticles were measured by Transmission Electron Microscope (TEM). The synthesis process was started by reacting HAuCl4 and the reducing agents in an aqueous solution at 86 ºC. The initial gold concentration, reducing agents concentration and pH were varied in order to obtain the optimum condition. In the optimum condition, the results showed that p-aminosalicylic acid containing both hydroxyl and amino groups performed higher reduction ability compared to p-aminobenzoic acid that only containing an amino group. Reducing agents which have a hydroxyl group (p-aminosalicylic acid) could produce AuNPs with a smaller concentration of HAuCl4 than p-aminobenzoic acid. Gold nanoparticles that were synthesized with p-aminosalicylic acid were more stable and had a smaller particle size compared to its counterpart that is synthesized with p-aminobenzoic acid.


gold nanoparticles; p-aminosalicylic acid; p-aminobenzoic acid

Full Text:

Full Text PDF


[1] Puddephat, R.J., 2008, Modern Supramolecular Gold Chemistry: GoldMetal Interactions and Applications, (Eds.) Laguna, A., Wiley-VCH, Weinheim, John Wiley & Sons, Inc.

[2] Chen, H., and Schluesener, H.J., 2008, Nanosilver: A nanoproduct in medical application, Toxicol. Lett., 176 (1), 1–12.

[3] Regiel-Futyra, A., Kus-Liśkiewicz, M., Sebastian, V., Irusta, S., Arruebo, M., Stochel, G., and Kyzioł, A., 2015, Development of nontoxic chitosan-gold nanocomposites as efficient antibacterial materials, ACS Appl. Mater. Interfaces, 7 (2), 1087–1099.

[4] Aragay, G., Pino, F., and Merkoçi, A., 2012, Nanomaterials for sensing and destroying pesticides, Chem. Rev., 112 (10), 5317–5338.

[5] Corthey, G., Giovanetti, L.J., Ramallo-López, J.M., Zelaya, E., Rubert, A.A., Benitez, G.A., Requejo, F.G., Fonticelli, M.H., and Salvarezza, R.C., 2010, Synthesis and characterization of gold@gold(I)−thiomalate core@shell nanoparticles, ACS Nano, 4 (6), 3413–3421.

[6] Pal, T., Sau, T.K., and Jana, N.R., 1997, Reversible formation and dissolution of silver nanoparticles in aqueous surfactant media, Langmuir, 13 (6), 1481–1485.

[7] Goia, D.V., and Matijević, E., 1998, Preparation of monodispersed metal particles, New J. Chem., 22 (11), 1203–1215.

[8] Munro, C.H., Smith, W.E., Garner, M., Clarkson, J., and White, P.C., 2002, Characterization of the surface of a citrate-reduced colloid optimized for use as a substrate for surface-enhanced resonance Raman scattering, Langmuir, 11 (10), 3712–3720.

[9] Rodríguez-Sánchez, L., Blanco, M.C., and López-Quintela, M.A., 2000, Electrochemical Synthesis of Silver Nanoparticles, J. Phys. Chem. B, 104 (41), 9683–9688.

[10] Zhu, J., Liu, S., Palchik, O., Koltypin, Y., and Gedanken, A., 2000, Shape-controlled synthesis of silver nanoparticles by pulse sonoelectrochemical methods, Langmuir, 16 (16), 6396–6399.

[11] Pastoriza-Santos, I., and Liz-Marzán, L.M., 2002, Formation of PVP-protected metal nanoparticles in DMF, Langmuir, 18 (7), 2888–2894.

[12] Barman, G., Maiti, G., and Laha, J.K., 2013, Bio-fabrication of gold nanoparticles using aqueous extract of red tomato and its use as a colorimetric sensor, Nanoscale Res. Lett., 8 (1), 181–190.

[13] Lu, Y.C., and Chou, K.S., 2008, A simple and effective route for the synthesis nano-silver colloidal dispersions, J. Chin. Inst. Chem. Eng., 39 (6), 673–678.

[14] Leiva, A., Bonardd, S., Pino, M., Saldias, M., Kortaberria, G., and Radić, D., 2015, Improving the performance of chitosan in the synthesis and stabilization of gold nanoparticles, Eur. Polym. J., 68, 419–431.

[15] Bin Ahmad, M., Lim, J.J., Shameli, K., Ibrahim, N.A., and Tay, M.Y., 2011, Synthesis of silver nanoparticles in chitosan, gelatin and chitosan/gelatin bionanocomposites by a chemical reducing agent and their characterization, Molecules, 16 (9), 7237–7248.

[16] Haiss, W., Thanh, N.T.K., Aveyard, J., and Fernig, D.G., 2007, Determination of size and concentration of gold nanoparticles from UV−vis spectra, Anal. Chem., 79 (11), 4215−4221.

[17] Di Carlo, G., Curulli, A., Toro, R.G., Bianchini, C., De Caro, T., Padeletti, G., Zane, D., and Ingo, G.M., 2012, Green synthesis of gold−chitosan nanocomposites for caffeic acid sensing, Langmuir, 28 (12), 5471−5479.

[18] Aziz, M.A., Kim, J.P., and Oyama, M., 2014, Preparation of monodispersed carboxylate-functionalized gold nanoparticles using pamoic acid as a reducing and capping reagent, Gold Bull., 47 (1-2), 127–132.

[19] Nadh, R.V., Sundar, B.S., and Radhakrishnamurti, P.S., 2001, Kinetics of oxidation of aniline, p-aminobenzoic acid, and p-nitroaniline by 2,6-dichloroquinone-4-chloroimide, Russ. J. Phys. Chem., 75 (2), 229–233.

[20] Bala, R., Sharma, R.K., and Wangoo, N., 2016, Development of gold nanoparticles-based aptasensor for the colorimetric detection of organophosphorus pesticide phorate, Anal. Bioanal. Chem., 408 (1), 333–338.

[21] Nita, R., Trammell, S.A., Ellis, G.A., Moore, M.H., Soto, C.M., Leary, D.H., Fontana, J., Talebzadeh, S.F., and Knight, D.A., 2016, Kinetic analysis of the hydrolysis of methyl parathion using citrate-stabilized 10 nm gold nanoparticles, Chemosphere, 144, 1916–1919.

[22] D’souza, S.L., Pati, R.K., and Kailasa, S.K., 2014, Ascorbic acid functionalized gold nanoparticles as a probe for colorimetric and visual read-out determination of dichlorvos in environmental samples, Anal. Methods, 6 (22), 9007–9014.

[23] Susanthy, D., Fadliah, Wahyuni, E.T., and Santosa, S.J., 2017, Synthesis of silver nanoparticles using o-hydroxybenzoic acid, p-hydroxybenzoic acid, and o,p-dihydroxybenzoic acids as reducing agents, Mater. Sci. Forum, 901, 26–31.


Article Metrics

Abstract views : 473 | views : 430

Copyright (c) 2018 Indonesian Journal of Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.


Indonesian Journal of Chemisty (ISSN 1411-9420 / 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

Analytics View The Statistics of Indones. J. Chem.