Optimization on Supercritical CO2 Extraction Process to Improve the Quality of Patchouli Oil by Response Surface Methodology Approach

https://doi.org/10.22146/ijc.26605

Edi Priyo Utomo(1*), Marina Marina(2), Warsito Warsito(3), Egi Agustian(4)

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran, Malang 65145, Indonesia
(2) Department of Agroindustrial Technology, Faculty of Agriculture Technology, Brawijaya University, Jl. Veteran, Malang 65145, Indonesia
(3) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran, Malang 65145, Indonesia
(4) Research Center for Chemistry, Indonesian Institute of Sciences, Kawasan PUSPIPTEK, Serpong – 15314, South Tangerang, Banten, Indonesia
(*) Corresponding Author

Abstract


Until now, the patchouli oil is the largest commodity export of essential oil for Indonesia. But the price of patchouli oil is often unstable due to the quality of oil which fluctuates depending on the components. To improve the performance and quality of patchouli oil had been carried out the purification process using supercritical CO2 fluid extraction method. Optimization conditions of the extraction process using the approach of Response Surface Methodology (RSM) and the influence of independent variables include temperature (35–45 °C), pressure (80–150 atm) and the time of extraction (60–300 min), and then evaluated using a Box-Behnken Design (BBD). Analysis of the components in the extract was carried out by using GC-MS and GC-FID to detect the changes of compositions of the components containing the oil before and after the extraction process. The optimum condition of supercritical extraction within experimental range of the studied variables was at 38 °C, 146.35 atm and 74 min for predicted oil yield of 6.41% and at 41.45 °C, 135.17 atm and 252.62 min and the predicted patchouli alcohol content was 25.34%. The extracted oil is enriched with the main components such as patchouli alcohol compared to the original patchouli oil. The results of RSM evaluation showed consistency between the variables contained in the experiment and the prediction.

Keywords


supercritical CO2; patchouli oil; Response Surface Methods (RSM)

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References

[1] Standard Nasional Indonesia, 2006, Minyak Nilam, BSN, 1–15.

[2] Sulaiman, A., and Harsono, D., 2012, Pengaruh lama penyulingan dan komposisi bahan baku terhadap rendemen dan mutu minyak atsiri dari daun dan batang Nilam (Pogostemon cablin Benth), JRIHH, 4 (2), 16–21.

[3] Jauhari, G., and Fitri, M., 2014, Perbaikan sistem pendingin (condensor) alat penyulingan minyak nilam untuk meningkatkan rendemen, JIT, 8 (1), 28–33.

[4] Caputo, G., Fernández, I.G., Saldaña, M.D.A., and Galia, A., 2013, Advances and perspectives of supercritical fluid technology, J. Chem., 3, 17–20.

[5] Reis-Vasco, E.M.C., Coelho, J.A.P., Palavra, A.M.F., Marrone, C., and Reverchon, E., 2000, Mathematical modelling and simulation of pennyroyal essential oil supercritical extraction, Chem. Eng. Sci., 55 (15), 2917–2922.

[6] Danh, L.T., Truong, P., Mammucari, R., and Foster, N., 2010, Extraction of vetiver essential oil by ethanol-modified supercritical carbon dioxide, Chem. Eng. J., 165 (1), 26–34.

[7] Bocevska, M., and Sovová, H., 2007, Supercritical CO2 extraction of essential oil from yarrow, J. Supercrit. Fluids, 40 (3), 360–367.

[8] Ahmed, Z., Abdeslam-Hassan, M., Ouassila, L., and Danielle, B., 2012, Extraction and modeling of Algerian Rosemary essential oil using supercritical CO2: Effect of pressure and temperature, Energy Procedia, 18, 1038–1046.

[9] Conde-Hernández, L.A., Espinosa-Victoria, J.R., Trejo, A., and Guerrero-Beltrán, J.A., 2017, CO2-supercritical extraction, hydrodistillation and steam distillation of essential oil of rosemary (Rosmarinus officinalis), J. Food Eng., 200, 81–86.

[10] Zhao, T., Gao, F., Zhou, L., and Song, T.Y., 2013, Essential oil from Inula britannica extraction with SF-CO2 and its antifungal activity, J. Integr. Agric., 12 (10), 1791–1798.

[11] Suetsugu, T., Tanaka, M., Iwai. H., Matsubara, T., Kawamoto, Y., Saito, C., Sasaki, Y., Hoshino, M., Quitain, A.T., Sasaki, M., Sakamoto, J., and Goto, M., 2013, Supercritical CO2 extraction of essential oil from Kabosu (Citrus sphaerocarpa Tanaka) peel, Flavour, 2, 18.

[12] Liza, M.S., Rahman, R.A., Mandana, B., Jinap, S., Rahmat, R.A., Zaidul, I.S.M., and Hamid, A., 2012, Supercritical fluid extraction of bioactive flavonoid from Strobilanthes crispus (Pecah kaca) and its comparison with solvent extraction, Int. Food Res. J., 19 (2), 503–508.

[13] Reátegui, J.L.P., Machado, A.P.F., Barbero, G.F., Rezende, C.A., and Martínez, J., 2014, Extraction of antioxidant compounds from blackberry (Rubus sp.) bagasse using supercritical CO2 assisted by ultrasound, J. Supercrit. Fluids, 94, 223–233.

[14] Xia, L., You, J., Li, G., Sun, Z., and Suo, Y., 2011, Compositional and antioxidant activity analysis of zanthoxylum bungeanum seed oil obtained by supercritical CO2 fluid extraction, J. Am. Oil Chem. Soc., 88 (1), 23–32.

[15] Beckman, E.J., 2004, Supercritical and near-critical CO2 in green chemical synthesis and processing, J. Supercrit. Fluids, 28 (2-3), 121–191.

[16] Manan, Z.A., Lim, C.S., Rahman, N.S.A., and Alwi, S.R.W., 2008, Modelling of a new supercritical fluid extraction process for palm oil refining, Int. Conf. Sci. Technol. Appl. Ind. Educ., 1–7.

[17] Akanda, M.J., Sarker, M.Z., Ferdosh, S., Manap, M.Y., Rahman, N.N.A., and Kadir, M.O.A., 2012, Applications of supercritical fluid extraction (SFE) of palm oil and oil from natural sources, Molecules, 17 (2), 1764–1794.

[18] Goula, A.M., Ververi, M., Adamopoulou, A., and Kaderides, K., 2017, Green ultrasound-assisted extraction of carotenoids from pomegranate wastes using vegetable oils, Ultrason. Sonochem., 34, 821–830.

[19] Krishnaiah, D., Bono, A., Sarbatly, R., and Fadhilah, S., 2012, Supercritical fluid extraction of kernel cake, Am. J. Food Technol., 7 (3), 168–172.

[20] Mäki-Arvela, P., Hachemi, I., and Murzin, D.Y., 2014, Comparative study of the extraction methods for recovery of carotenoids from algae: Extraction kinetics and effect of different extraction parameters, J. Chem. Technol. Biotechnol., 89 (11), 1607–1626.

[21] Mićić, V., Novaković, D., Lepojević, Ž., Jotanović, M., Pejović, B., Dugić, P., and Petrović, Z., 2011, Supercritical fluid extraction with carbon dioxide at different pressures, Contemp. Mater., 1, 85–87.

[22] Xu, L., Zhan, X., Zeng, Z., Chen, R., Li, H., Xie, T., and Wang, S., 2011, Recent advances on supercritical fluid extraction of essential oils, Afr. J. Pharm. Pharmacol., 5 (9), 1196–1211.

[23] Shojaie, G.R., Shirazi, M.M.A., Kargari, A., and. Shirazi, M.J.A., 2010, Solubility prediction of supercritical fluids extraction by equations of state, J. Appl. Chem. Res., 13, 41–59.

[24] Cvjetko, M., Jokić, S., Lepojević, Ž., Vidović, S., Marić, B., and Redovniković, I.R., 2012, Optimization of the supercritical CO2 extraction of oil from rapeseed using response surface methodology, Food Technol. Biotechnol., 50 (2), 208–215.

[25] Leivisk, K., 2013, Introduction to Experiment Design, Table of Contents, University of Oulu Control Engineering Laboratory, http://www.itl.nist.gov/div898/handbook/, accessed at November 01, 2017.

[26] Shanock, L.R., Baran, B.E., Gentry, W.A., Pattison, S.C., and Heggestad, E.D., 2010, Polynomial regression with response surface analysis: A powerful approach for examining moderation and overcoming limitations of difference scores, J. Bus. Psychology, 25 (4), 543–554.

[27] Bezerra, M.A., Santelli, R.E., Oliveira, E.P., Villar, L.S., and Escaleira, L.A., 2008, Response surface methodology (RSM) as a tool for optimization in analytical chemistry, Talanta, 76 (5), 965–977.



DOI: https://doi.org/10.22146/ijc.26605

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