Adsorption of β-Carotene in Isopropyl Alcohol with Decolorized Activated Carbon as Model for β-Carotene Adsorption in Crude Palm Oil

Maria Ulfah(1*), Sri Raharjo(2), Pudji Hastuti(3), Purnama Darmadji(4)

(1) Department of Agricultural Technology, Institute of Agriculture STIPER, Jl. Nangka II, Maguwoharjo, Depok, Sleman 55282
(2) Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No. 1, Bulaksumur, Yogyakarta 55281
(3) Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No. 1, Bulaksumur, Yogyakarta 55281
(4) Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No. 1, Bulaksumur, Yogyakarta 55281
(*) Corresponding Author


The objective of this research was to characterize the porosity of decolorized activated carbon (DAC) and its ability to adsorb β-carotene as a preliminary study to characterize activated carbon as an adsorbent to adsorb carotene from crude palm oil. The capability of DAC to adsorb β-carotene was studied using isopropyl alcohol as a solvent in equilibrium conditions. The adsorption time was specified for 0.5 to 60 min, while the DAC optimum concentration for adsorption of β-carotene was determined at a concentration of 0.5 to 2.5%. Adsorption isotherm study was conducted for a β-carotene concentration of 100 to 500 ppm and at temperatures of 30, 40 and 50 °C at equilibrium time and DAC optimum concentration. The results showed that the DAC had a surface area of 1068.391 m2/g, total pore volume of 0.952 cc/g, mesopore volume of 0.528 cc/g (55.4%) and mesopore radius of 15.26 Å. Based on the levels of β-carotene adsorbed onto the DAC, the equilibrium was reached after adsorption of 5 min. Decolorized activated carbon with a concentration of 1.5% was able to adsorb β-carotene optimally. Adsorption of β-carotene at 40 °C for 60 min according to Freundlich models with the intensity of adsorption of 1.002 and adsorption capacity of 63.358 L/mg. While the adsorption of β-carotene at 50 °C for 60 min in accordance with the model of Langmuir, the adsorption capacity of 33.206 mg/g and the Langmuir adsorption equilibrium constant of 1.721 L/mg.


β-carotene; decolorized activated carbon; porosity; adsorption isotherm

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[1] Zeb, A., and Mehmood, S., 2004, Carotenoids Contents from Various Sources and Their Potential Health Applications, Pak. J. Nutr., 3 (3), 199–204.

[2] Silva, S.M., Sampaio, K.A., Ceriani, R., Verhé, R., Stevens, C., Greyt, W.D., and Meirelles, A.J.A., 2013, Adsorption of carotenes and phosphorus from palm oil onto acid activated bleaching earth: Equilibrium, kinetics and thermodynamics, J. Food Eng., 118 (4), 341–349.

[3] Terao, J., Minami, Y., and Bando, N., 2011, Singlet molecular oxygen-quenching activity of carotenoids: relevance to protection of the skin from photoaging, J. Clin. Biochem. Nutr., 48 (1), 57–62.

[4] Ariviani, S., Raharjo, S., and Hastuti, P., 2011, Potensi mikroemulsi β-karoten dalam menghambat fotooksidasi vitamin c sistem aqueous, Jurnal Teknologi dan Industri Pangan, 22, 33–40.

[5] Ariviani, S., Raharjo, S., Hastuti, P., 2011, Aplikasi mikroemulsi [1]β-karoten untuk menghambat kerusakan fotooksidatif vitamin c pada sari buah jeruk, Agritech, 31 (3), 180–189.

[6] Zeb, A., 2011, Effects of β-carotene on the thermal oxidation of fatty acids, Afr. J. Biotechnol., 10 (68), 15346–15352.

[7] Tang, G., 2010, Bioconversion of dietary provitamin A carotenoids to vitamin A in humans, Am. J. Clin. Nutr., 91 (5), 1468S–1473S.

[8] Lee, H.A., Park, S., and Kim, Y., 2013, Effect of β-carotene on cancer cell stemness and differentiation in SK-N-BE(2)C neuroblastoma cells, Oncol. Rep., 30 (4), 1869–1877.

[9] Othman, N., Manan, Z.A., Alwi, S.R.W., and Sarmidi, M.R., 2010, A review of extraction technology for carotenoids and vitamin e recovery from palm oil, J. Appl. Sci., 10 (12), 1187–1191.

[10] Muhammad, Choong, T.S.Y., Chuah, T.G., Yunus, R., and Yap, Y.H.T., 2010, Adsorption of β-carotene onto mesoporous carbon coated monolith in isopropyl alcohol and n-hexane solution: equilibrium and thermodynamic study, Chem. Eng. J., 164 (1), 178–182.

[11] Baharin, B.S., Rahman, K.A., Karim, M.I.A., Oyaizu, T., Tanaka, K., Tanaka, Y., and Takagi, S., 1998, Separation of palm carotene from crude palm oil by adsorption chromatography with a synthetic polymer adsorbent, J. Am. Oil Chem. Soc., 75 (3), 399–404.

[12] Barό, A.M., Hla, S.W., and Rieder, K.H., 2003, LT-STM study of self-organization of β-carotene molecular layers on Cu (1 1 1), Chem. Phys. Lett., 369 (1-2), 240–247.

[13] Ulfah, M., Raharjo, S., Hastuti, P., and Darmadji, P., The potential of palm kernel shells activated carbon as an adsorbent for β-carotene recovery from crude palm oil, 2016, AIP Conf. Proc., 1755 (1), 130016.

[14] Dauqan, E.M.A., Sani, H.A, Abdullah, A., and Kasim, Z.M., 2011, Fatty acids composition of four different vegetable oils (red palm olein, palm olein, corn oil and coconut oil) by gas chromatography, International Proceedings of Chemical, Biological and Environmental Engineering (IPCBEE), International Association of Computer Science and Information Technology (IACSIT) Press, Singapore, 31–34.

[15] Lambert, J.B., Shurvell, H.F., Cooks, R.G., 1987, Introduction to Organic Spectroscopy, 1st ed., Macmillan Publication, New York.

[16] Coates, J., 2000, “Interpretation of infrared spectra, a practical approach” in Encyclopedia of Analytical Chemistry, Meyers, R.A. (Ed.), John Wiley & Sons Ltd., Chichester, 10815–10837.

[17] Muslich, Suryadarma, P., and Hayuningtyas, R.I.R., 2009, Kinetika adsorpsi isotermal β-karoten dari olein sawit kasar dengan menggunakan bentonit, Journal Teknologi Industri Pertanian, 19 (2), 93–100.

[18] Ferhan Çeçen, F., and Aktaş, Ö, 2011, Activated Carbon for Water and Wastewater Treatment: Integration of Adsorption and Biological Treatment, Wiley-VCH Verlag GmbH & Co. KGaA, Germany.

[19] Mak, S.M., Tey, B.T., Cheah, K.Y., Siew, W.L., and Tan, K.K., 2009, Porosity characteristics and pore developments of various particle sizes palm kernel shells activated carbon (PKSAC) and its potential applications, Adsorption, 15 (5), 507–519.

[20] Lim, W.C., Srinivasakannan, C., and Balasubramanian, N., 2010, Activation of palm shells by phosphoric acid impregnation for high yielding activated carbon, J. Anal. Appl. Pyrolysis, 88 (2), 181–186.

[21] Okoniewska, E., Lach, J., Ociepa, E., and Stępniak, L., 2013, Removal of selected organic compounds on modified activated carbons, Environ. Prot. Eng., 39 (2), 135–144.

[22] Karabulut, I., Topcu, A., Akmil-Basar, C., Onal, Y., and Lampi, A.M., 2008, Obtaining butter oil triacylglycerols free from ß-carotene and -tocopherol via activated carbon adsorption and alumina-column chromatography treatments, J. Am. Oil Chem. Soc., 85 (3), 213–219.

[23] Sabah, E., Ҫinar, M., and Ҫelik, M.S., 2007, Decolorization of vegetable oils: Adsorption mechanism of β-carotene on acid-activated sepiolite, Food Chem., 100 (4), 1661–1668.

[24] Wu, X.W., Ma, H.W., Zhang, L.T., and Wang, F.J., 2012, Adsorption properties and mechanism of mesoporous adsorbents prepared with fly ash for removal of Cu(II) in aqueous solution, Appl. Surf. Sci., 261, 902–907.

[25] Badr, N., and Al-Qahtani, K.M., 2013, Treatment of wastewater containing arsenic using Rhazya stricta as a new adsorbent, Environ. Monit. Assess., 185 (12), 9669–9681.

[26] Fettouche, S., Tahiri, M., Madhouni, R., Cherkaoui, O., 2015, Removal of reactive dyes from aqueous solution by adsorption onto alfa fibers powder, J. Mater. Environ. Sci., 6 (1), 129–137.


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