Antioxidant Activity of Syzygium polyanthum Extracts

https://doi.org/10.22146/ijc.23545

Mutia Devi Hidayati(1), Taslim Ersam(2), Kuniyoshi Shimizu(3), Sri Fatmawati(4*)

(1) Department of Chemistry, Sepuluh Nopember Institute of Technology (ITS), Jl. Arief Rahman Hakim, Sukolilo, Surabaya 60111
(2) Department of Chemistry, Sepuluh Nopember Institute of Technology (ITS), Jl. Arief Rahman Hakim, Sukolilo, Surabaya 60111
(3) Department of Agro-environmental Science, Faculty of Agriculture, Kyushu University, Fukuoka, 812-8581
(4) Department of Chemistry, Sepuluh Nopember Institute of Technology (ITS), Jl. Arief Rahman Hakim, Sukolilo, Surabaya 60111
(*) Corresponding Author

Abstract


Antioxidant activities of Syzygium polyanthum leaves extracts (methanol, ethyl acetate, dichloromethane and n-hexane) were evaluated by using DPPH (2,2-diphenyl-2-picrylhydrazyl) and ABTS (2,2’-azinobis (3-ethylbenzothiazole-6-sulfonic acid) methods. The methanol extract showed the highest antioxidant activity of DPPH assay among extract with IC50 value of 44.35 mg/mL. In addition, methanol extract also showed the highest antioxidant activity of ABTS assay among extracts with IC50 value of 17.69 mg/mL. This study indicated that the methanol extract of S. polyanthum leaves is potential as antioxidant.

Keywords


antioxidant activity; DPPH; ABTS; Syzygium polyanthum; salam leaves

Full Text:

Full Text Pdf


References

[1] Sarma, A.D., Mallick, A.R., and Ghosh, A.K., 2010, Free Radicals and Their Role in Different Clinical Conditions: An Overview, Int. J. Pharma Sci. Res., 1 (3), 185–192.

[2] Fearon, E.R., 1997, Human cancer syndromes: clues to the origin and nature of cancer, Science, 278 (5340), 1043–1050.

[3] Biskup, I., Golonka, I., Gamian, A., and Sroka, Z., 2013, Antioxidant activity of selected phenols estimated by ABTS and FRAP methods, Postepy Hig. Med. Dosw., 67, 958–963.

[4] Ismiyati, 2013, Aktivitas antihipertensi ekstrak etanol daun salam (Syzygium polyantha [wigth] walp) pada tikus wistar, profil kromatografi lapis tipis serta penetapan kandungan fenolik total dan flavonoid totalnya, Thesis, UGM, Yogyakarta.

[5] Kusuma, I.W., Kuspradini, H., Arung, E.T., Aryani, F., Min, Y.H., Kim, J.S., and Kim, Y.U., 2011, Biological activity and phytochemical analysis of three Indonesian medicinal plants, Murraya koenigii, Syzygium polyanthum and Zingiber purpurea, J. Acupunct. Meridian Stud., 4 (1), 75–79.

[6] Nuratmi, B., Winarno, M.W., and Sundari, S., 1999, Khasiat daun salam (Eugenia polyantha Wight) sebagai antidiare pada tikus putih, Media Litbangkes “Obat Asli Indonesia”, 8 (3-4), 14–17.

[7] Darusman, L.K., Wahyuni, T.W., and Alwi, F., 2013, Acetylcholinesterase inhibition and antioxidant activity of Syzygium cumini, S. aromaticum and S. polyanthum from Indonesia, J. Biol. Sci., 13 (5), 412–416.

[8] Arumugam, B., Manaharan, T., Heng, C.K., Kuppusamy, U.R., and Palanisamy, U.D., 2014, Antioxidant and antiglycemic potentials of a standardized extract of Syzygium malaccense, LWT Food Sci. Technol., 59 (2), 707–712.

[9] Brand-Williams, W., Cuvelier, M.E., and Berset, C., 1995, Use of a free radical method to evaluate antioxidant activity, LWT Food Sci. Technol., 28 (1), 25–30.

[10] Dudonné, S., Vitrac, X., Coutière, P., Woillez, M., and Mérillon, J.M., 2009, Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays, J. Agric. Food Chem., 57 (5), 1768–1774.

[11] Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., and Rice-Evans, C., 1999, Antioxidant activity applying an improved ABTS radical cation decolorization assay, Free Radic. Biol. Med., 26 (9-10), 1231–1237.

[12] Shalaby, E.A., and Shanab, S.M.M., 2012, Comparison of DPPH and ABTS assays for determining antioxidant potential of water and methanol extracts of Spirulina platensis, Indian J. Geo Mar. Sci., 42 (25), 556–564.

[13] Żurowska, D.M., and Wenta, W., 2012, A comparison of ABTS and DPPH methods for assessing the total antioxidant capacity of human milk, Acta Sci. Pol., Technol. Aliment., 11 (1), 83–89.

[14] Prior, R.L., Wu, X., and Schaich, K., 2005, Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements, J. Agric. Food Chem., 53 (10), 4290–4302.

[15] Sultana, B., Anwar, F., and Przybylski, R., 2007, Antioxidant activity of phenolic components present in barks of Azadirachta indica, Terminalia arjuna, Acacia nilotica, and Eugenia jambolana Lam. trees, Food Chem., 104 (3), 1106–1114.



DOI: https://doi.org/10.22146/ijc.23545

Article Metrics

Abstract views : 1978 | views : 2186


Copyright (c) 2017 Indonesian Journal of Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 


Indonesian Journal of Chemisty (ISSN 1411-9420 / 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

Web
Analytics View The Statistics of Indones. J. Chem.