Removal of Sulphate and Manganese on Synthetic Wastewater in Sulphate Reducing Bioreactor Using Indonesian Natural Zeolite

https://doi.org/10.22146/ijc.22710

Endah Retnaningrum(1*), Wahyu Wilopo(2)

(1) Faculty of Biology, Universitas Gadjah Mada, Indonesia
(2) Geological Engineering Department, Faculty of Engineering, Universitas Gadjah Mada, Jl. Grafika No. 2, Bulaksumur, Yogyakarta 55281
(*) Corresponding Author

Abstract


The present research was conducted to investigate sulphate and manganese removal from synthetic wastewater. The continuous laboratory scale of down-flow fluidized-bed reactor (DFBR) using sulphate reducing bacteria (SRB) consortium and Indonesian natural zeolite as a bacterial support material was designed. At 9 days operation, maximum sulphate and manganese removal was observed to be 23% and 15.4%, respectively. The pH values were also changed to neutral. The population of SRB increased which effect on the raising of their activity for removing sulphate and manganese. Using the scanning electronic microscopy (SEM), it was observed that natural zeolite possesses excellent physical characteristics as a bacterial support material in DFBR. The imaging SEM result of SRB consortium on zeolite surface clearly showed the developed SRB biofilm on that particle. Analysis result of EDX confirmed that manganese was precipitated as manganese–sulfides.


Keywords


DFBR; SRB consortium; natural zeolite; SEM-EDX; manganese-sulfides

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References

[1] Song, H., Yim, G.J., Ji, S.W., Neculita, C.M., and Hwang, T., 2012, Pilot-scale passive bioreactors for the treatment of acid mine drainage: Efficiency of mushroom compost vs. mixed substrates for metal removal, J. Environ. Manage., 111, 150–158.

[2] Neculita, C.M., and Zagury, G.J., 2008, Biological treatment of highly contaminated acid mine drainage in batch reactors: Long-term treatment and reactive mixture characterization, J. Hazard. Mater., 157 (2-3), 358–366.

[3] Boucher, A.M., and Watzin, M.C., 1999, Toxicity identification evaluation of metal-contaminated sediments using an artificial pore-water containing dissolved organic carbons, Environ. Toxicol. Chem., 18 (3), 509–518.

[4] Doyle, C.J., Pablo, F., Lim, R.P., and Hyne, R.V. 2003, Assessment of metal toxicity in sediment pore water from Lake Macquarie, Australia, Arch. Environ. Contam. Toxicol., 44 (3), 343–350.

[5] Nagpal, S., Chuichulcherm, S., Peeva, L., and Livingston, A., 2000, Microbial sulfate-reduction in a liquid-solid fluidized bed reactor, Biotechnol. Bioeng., 70 (4), 370–380.

[6] Steed, V.S., Suidan, M.T., Gupta, M., Miyahara, T., Acheson, C.M., and Sayles, G.D., 2000, Development of a sulfate-reducing biological process to remove heavy metals from acid mine drainage, Water Environ. Res., 72 (5), 530–535.

[7] Kaksonen, H., Franzmann, P.D., and Puhakka, J.A., 2004, Effects of hydraulic retention time and sulfide toxicity on ethanol and acetate oxidation in sulfate-reducing metal-precipitating fluidized bed reactor, Biotechnol. Bioeng., 86 (3), 332–343.

[8] Bayrakdar, A., Sahinkaya, E., Gungor, M., Uyanik, S., and Atasoy, A.D., 2009, Performance of sulfidogenic anaerobic baffled reactor (ABR) treating acidic and zinc-containing wastewater, Bioresour. Technol., 100 (19), 4354–4360.

[9] Nevatalo, L.M., Mäkinen, A.E., Kaksonen, A.H., and Puhakka, J.A., 2010, Biological hydrogen sulfide production in an ethanol–lactate fed fluidized-bed bioreactor, Bioresour. Technol., 101 (1), 276–284.

[10] Kolmert, Å., and Johnson, D.B., 2001, Remediation of acidic wastewaters using immobilised, acidophilic sulfate-reducing bacteria, J. Chem. Technol. Biotechnol., 76, 836–843.

[11] Tsukamoto, T.K., Killion, H.A., and Miller, G.C., 2004, Column experiments for microbiological treatment of acid mine drainage: low-temperature, low pH and matrix investigations, Water Res., 38 (6), 1405–1418.

[12] Gibert, O., de Pablo, J., Cortina, J.L., and Ayora, C., 2004, Chemical characterization of natural organic substrates for biological mitigation of acid mine drainage, Water Res., 38 (19), 4186–4196.

[13] Sheoran, A.S., and Sheoran, V., 2006, Heavy metal removal mechanism of acid mine drainage in wetlands: A critical review, Miner. Eng., 19 (2), 105–116.

[14] Ouki, S.K., Cheeseman, C.R., and Perry, R., 1994, Natural zeolite utilization in pollution control: a review of applications to metals' effluents, J. Chem. Technol. Biotechnol., 59 (2), 121–126.

[15] Baker, H.M., Massadeh, A.M., and Younes, H.A., 2009, Natural Jordanian zeolite: Removal of heavy metal ions from water samples using column and batch methods, Environ. Monit. Assess., 157 (1-4), 319–330.

[16] Shi, W., Shao, H., Li, H., Shao, M., and Du, S., 2009, Progress in the remediation of hazardous heavy metal-polluted soils by natural zeolite, J. Hazard. Mater., 170 (1), 1–6.

[17] Sánchez, E., and Roque-Malherbe, R.M.A., 1987, Zeolite as support material in anaerobic wastewater treatment, Biotechnol. Lett., 9 (9), 671–672.

[18] Fernández, N., Fdz-Polanco, F., Montalvo, S.J., and Toledano, D., 2001, Use of activated carbon and natural zeolite as support materials in an anaerobic fluidized bed reactor, for vinasse treatment, Water Sci. Technol., 44 (4), 1–6.

[19]Hrenovic, J., Ivankovic, T., and Tibljas, D., 2009, The effect of mineral carrier composition on phosphate-accumulating bacteria immobilization, J. Hazard. Mater., 166 (2-3), 1377–1382.

[20] Postgate, J.R., 1984, The Sulphate Reducing Bacteria, 2nd Ed., University Press, Cambridge, UK, 20–30.

[21] Gallegos-Garcia, M., Celis, L.B., Rangel-Méndez, R., and Razo-Flores, E., 2009, Precipitation and recovery of metal sulfides from metal containing acidic wastewater in a sulfidogenic down-flow fluidized bed reactor, Biotechnol. Bioeng., 102 (1), 91–99.

[22] Kolmert, Å., Wikström, P., and Hallberg, K.B., 2000, A fast and simple turbidimetric method for the determination of sulfate in sulfate-reducing bacterial cultures, J. Microbiol. Methods, 41 (3), 179–184.

[23] Cardell, C., and Guerra, I., 2016, An overview of emerging hyphenated SEM-EDX and Raman spectroscopy systems: Applications in life, environmental and materials sciences, TrAC, Trends Anal. Chem., 77, 156–166.

[24] Borja, R., and Banks, C.J., 1994, Kinetics of anaerobic digestion of soft drink wastewater in immobilized cell bioreactors, J. Chem. Technol. Biotechnol., 60 (3), 327–334.

[25] Borja, R., Sánchez, E., Weiland, P., Travieso, L., and Martín, A., 1993, Effect of natural zeolite support on the kinetics of cow manure anaerobic digestion, Biomass Bioenergy, 5 (5), 395–400.

[26] Milán, Z., Villa, P., Sánchez, E., Montalvo, S., Borja, R., Ilangovan, K., and Briones, R., 2003, Effect of natural and modified zeolite addition on anaerobic digestion of piggery waste, Water Sci. Technol., 48 (6), 263–269.

[27] Ríos, C.A., Williams, C.D., and Roberts, C.L., 2008, Removal of heavy metals from acid mine drainage (AMD) using coal fly ash, natural clinker and synthetic zeolites, J. Hazard. Mater., 156 (1-3), 23–35.

[28] Motsi, T., Rowson, N.A., and Simmons, M.J.H., 2009, Adsorption of heavy metals from acid mine drainage by natural zeolite, Int. J. Miner. Process., 92 (1-2), 42–48.

[29] Sahinkaya, E., and Yucesoy, Z., 2010, Biotreatment of acidic zinc- and copper-containing wastewater using ethanol-fed sulfidogenic anaerobic baffled reactor, Bioprocess Biosyst. Eng., 33 (8), 989–997.

[30] Sahinkaya, E., 2009, Biotreatment of zinc-containing wastewater in a sulfidogenic CSTR: Performance and artificial neural network (ANN) modelling studies, J. Hazard. Mater., 164 (1), 105–113.

[31] Kaksonen, A.H., Franzmann, P.D., and Puhakka, J.A., 2003, Performance and ethanol oxidation kinetics of a sulfate-reducing fluidized-bed reactor treating acidic metal-containing wastewater, Biodegradation, 14 (3), 207–217.

[32] Sahinkaya, E., and Gungor, M., 2010, Comparison of sulfidogenic up-flow and down-flow fluidized-bed reactors for the biotreatment of acidic metal-containing wastewater, Bioresour. Technol., 101 (24), 9508–9514.

[33] Yamashita, T., Yamamoto-Ikemoto, R., and Zhu, J., 2011, Sulfate-reducing bacteria in a denitrification reactor packed with wood as a carbon source, Bioresour. Technol., 102 (3), 2235–2241.

[34] Fernández, N., Montalvo, S., Guerrero, L., Sánchez, E., Cortés, I., and Travieso, L., 2007, Anaerobic fluidized bed reactor application to tropical fruit wine effluent, Water Sci. Technol., 56 (2), 33–38.

[35] Nikolaeva, S., Sánchez, E., Borja, R., Raposo, F., Colmenarejo, M.F., Montalvo, S., and Jiménez-Rodríguez, A.M., 2009, Kinetics of anaerobic degradation of screened dairy manure by upflow fixed bed digesters: Effect of natural zeolite addition, J. Environ. Sci. Health. Part A Toxic/Hazard. Subst. Environ. Eng., 44 (2), 146–150.

[36] Quintelas, C., Rocha, Z., Silva, B., Fonseca, B., Figueiredo, H., and Tavares, T., 2009, Biosorptive performance of an Escherichia coli biofilm supported on zeolite NaY for the removal of Cr(VI), Cd(II), Fe(III) and Ni(II), Chem. Eng. J., 152 (1), 110–115.

[37] Quintelas, C., Rocha, Z., Silva, B., Fonseca, B., Figueiredo, H., and Tavares, T., 2009, Removal of Cd(II), Cr(VI), Fe(III) and Ni(II) from aqueous solutions by an E. coli biofilm supported on kaolin, Chem. Eng. J., 149 (1-3), 319–324.

[38] Lin, Y.H., Wu, C.L., Li, H.L., and Hsu, C.H., 2011, Verification of model for adsorption and reduction of chromium(VI) by Escherichia coli 33456 using chitosan bead as a supporting medium, Appl. Math. Modell., 35 (6), 2736–2751.

[39] Quintelas, C., Rocha, Z., Silva, B., Fonseca, B., Figueiredo, H., and Tavares, T., 2009, Removal of Cd(II), Cr(VI), Fe(III) and Ni(II) from aqueous solutions by an E. coli biofilm supported on kaolin, Chem. Eng. J., 149 (1-3), 319–324.

[40] Lin, Y.H., Wu, C.L., Li, H.L., and Hsu, C.H., 2011, Verification of model for adsorption and reduction of chromium(VI) by Escherichia coli 33456 using chitosan bead as a supporting medium, Appl. Math. Modell., 35 (6), 2736–2751.



DOI: https://doi.org/10.22146/ijc.22710

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