SPECTROSCOPIC AND PHYSICOCHEMICAL METHODS FOR STUDYING THE INTERACTION OF METALLOPORPHYRIN WITH DNA

https://doi.org/10.22146/ijc.21907

Hidenari Inoue(1*)

(1) Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522
(*) Corresponding Author

Abstract


In recent years studies on the interaction of porphyrin with DNA have received much attention because of the importance in DNA-probing and photodynamic therapy of cancer. A variety of spectroscopic methods, e.g. NMR, ESR, Mössbauer, UV-visible absorption, circular dichroism (CD), magnetic circular dichroism (MCD), IR and Raman spectroscopy, have been employed for studying interactions between porphyrin and DNA. Of these spectroscopic methods, only a few instrumental analytical techniques applicable to an aqueous buffer solution of DNA have been particularly developed to investigate porphyrin-DNA interactions. On the other hand, a number of physicochemical methods, e.g. gel electrophoresis, melting temperature measurements and hydrodynamic methods such as viscosity and sedimentation measurements, have been also used for determining the binding modes of porphyrin to DNA. The present lecture will focus on the application of visible absorption, CD and MCD spectroscopy as well as melting temperature and viscosity measurements to studies of porphyrin-DNA interactions.

 


Full Text:

Full Text PDF



DOI: https://doi.org/10.22146/ijc.21907

Article Metrics

Abstract views : 231 | views : 239


Copyright (c) 2010 Indonesian Journal of Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 


Indonesian Journal of Chemisty (ISSN 1411-9420 / 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

Web
Analytics View The Statistics of Indones. J. Chem.