Gemini Alam(1*), Puji Astuti(2), Subagus Wahyuono(3), Dinar Sari(4), Mark T Hamman(5)

(1) Department of Pharmacy, Faculty of Mathematic and Natural Sciences, Hasanuddin University, Makassar
(2) Pharmaceutical Biology Department, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta
(3) Pharmaceutical Biology Department, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta
(4) Biology Department, Faculty of Mathematic and Natural Sciences, Sebelas Maret University, Surakarta
(5) Department of Pharmacognosy, Faculty of Pharmacy, Mississippi University
(*) Corresponding Author


Two alkaloid compounds have been isolated from sponge Petrosia sp collected from Bunaken Bay Menado. These compounds have been found to inhibit several human cancer cell proliferation in vitro. Structure elucidation is needed to further characterize these bioactive compounds. The structure is determined by analyzing Ultra Violet (UV), Infra Red (IR), Mass Spectra (MS) and Nuclear Magnetic Resonance (NMR) spectra. Based on spectroscopic data, Thin Layer Chromatography (TLC) co-chromatograph with authentic samples and comparison with reported data the two compounds were identified as Manzamine A and 8-OH Manzamine A, respectively.


Manzamine A; 8-OH Manzamine A; Petrosia sp; structure elucidation

Full Text:

Full Text PDF


[1]   Anonim, 2004, Bidi, 2, 4

[2]   Bergquist, P.R., 1978, Sponges, Hutchinson and Company, London, p.268

[3]   Edrada, R.U., Proksch, P., Wray, V., Witte, L., Muller, W.E.G., and Van Soest, R.W.M., 1996, J.Nat.Prod., 59, 1056-1060

[4]   Ang, K.K.H., Holmes, M.J., Higa, T., Hamann, M.T., and Kara, U.A.K., 2000, Antimicrob. Agents Chemother., 1645-1649

[5]   El Sayed, K.A., Kelly, M., Kara, U.A.K., Ang, K.K.H., Dunbar, D.C., Khan, A.A., and Hamann, M.T., 2001, J.Am. Chem.Soc., 123,1804-1808

[6]   Breton, J.J., and Chabot-Fletcher, M.C., 1997, J. Pharmacol. Exp. Ther., 282, 459

[7]   Nakamura, H., Deng, S., Kobayashi, J., Ohizumi, Y., Tomotake, Y., Matsuzaki, T., Hirata, Y., 1987, Tetrahed. Lett., 28, 621

[8]   Tasdemir D., Mangalindan, G.C., Concepcion, G.P., Verbitski, S.M., Rabindran, S., Miranda, M., Greenstein, M., Hooper, J.N., Harper, M.K., and Ireland, C.M., 2002, , J. Nat. Prod., 65 (2), 210-214

[9]   Alam, G., Sari, D., dan  Astuti, P., 2003, Majalah Obat Tradisional, 8, 25, 20-24

[10] Astuti, P., Alam, G., Wahyuningsih, M.S.H., Sari, D., and Wahyuono, S., 2005a, Majalah Farmasi Indonesia, 16(1), 58-62

[11] Astuti, P., Alam, G., Junaedi, S., Ikawati, M., and Wahyuono, S., 2005b., Media Farmasi, submitted

[12] Astuti, P., Alam, G., Junaedi, S., Ikawati, M., and Wahyuono, S., 2005c, Indonesian Journal of Biotechnology, submitted

[13] Ichiba, T., Sakai, R., Kohmoto, S., and Saucy, G., 1988, Tetrahed. Lett., 29, 25, 3083-3086

[14] Ichiba, T., Corgiat, J.M., and Scheuer, P.J., 1994, J. Nat.Prod., 57, 1, 168-170

[15] Silverstein, R.M., and Webster, F.X., 1997, Spectroscopic Identification of Organic Compounds, 6th edition, John Wiley & Sons, New York


Article Metrics

Abstract views : 1032 | views : 1393

Copyright (c) 2010 Indonesian Journal of Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.


Indonesian Journal of Chemistry (ISSN 1411-9420 / 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

Analytics View The Statistics of Indones. J. Chem.