* Corresponding Author
DETERMINATION of OPTIMUM CONDITION of PAPAIN ENZYME FROM PAPAYA VAR JAVA (Carica papaya)
https://doi.org/10.22146/ijc.21822 Aline Puspita Kusumadjaja(1*), Rita Puspa Dewi(2)
(1) Chemistry Department, Faculty of Mathematics and Natural Sciences State University of Surabaya, Surabaya
(2) Chemistry Department, Faculty of Mathematics and Natural Sciences State University of Surabaya, Surabaya
(*) Corresponding Author
Abstract
A study to investigate the optimum condition of papain enzyme has been carried out. The condition that are investigated are pH and temperature, based on measurement of enzyme activity which is defined as mmole tyrosin that are released in reaction between papain enzyme and casein as substrat per minute. In this research, the papain enzyme was isolated from pepaya burung varietas Java. The enzyme was partially purified by precipitation method using 30% - 50% saturated acetone. The result showed that the optimum conditions of papain enzyme are in pH 6 with activity 2,606 U/mL, and temperature at 50 oC with activity 2,469 U/mL.
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[1] Stryer, L., 1988, Biochemistry, 3rd ed., W. H. Freeman and Company, New York
[2] Lehninger, A.L., 1994, Dasar-Dasar Biokimia, (terj. M. Thenawijaya), Jilid 1, Erlangga, Jakarta
[3] Sadikin, M., 2002, Biokimia Enzim, terbitan ke 1, Widya Medika, Jakarta
[4] Winarno, F.G., 1986, Enzim Pangan, Jakarta, Gramedia
[5] Aisyah, 2002, Manfaat Getah Pepaya, http://www.bio/papain.html
[6] Darwis, A.A., dan Sukara, E., 1990, Isolasi, Purifikasi dan Karakterisasi Enzim, Pusat Antar Universitas Bioteknologi, Institut Pertanian Bogor
[7] Sumartha, I.G., 1990, Oryza, XIV, 25, 10
[8] Murray, R.K., Ganner, D.K., Mayes, P.A., and Rodwell, V.W., 1997, Biokimia Harper, (terj. Andry Hartono), terbitan ke 24, Penerbit Buku Kedokteran EGC, Jakarta
[9] Bergmeyer, H.U., and Grassl, M., 1983, Methods of Enzymatic Analysis, Vol 2, Verlag Chemie, Weinheim
[10] Bollag, D.M., Rozycki, M.D., and Edelstein, S.J., 1996, Protein Methods, 2nd ed., A John Wiley & Sons, Inc., New York
[11] Cornely, K., Keravou, J., and Macvier, T., 1999, J. Chem. Ed., 76, 5, 644
[12] Wirahadikusumah, M., 1981, Biokimia Protein, Enzim dan Asam Nukleat, terbitan ke 2, ITB, Bandung
DOI: https://doi.org/10.22146/ijc.21822
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Indonesian Journal of Chemistry (ISSN 1411-9420 /e-ISSN 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.