Nurul Hidayat Aprilita(1*), Rania Bakry(2), Christian W Huck(3), Guenther K Bonn(4)

(1) Chemistry Department, Faculty of Mathematics and Natural Sciences, Gadjah Mada University,
(2) Institute of Analytical Chemistry and Radiochemistry, University of Innsbruck,
(3) Institute of Analytical Chemistry and Radiochemistry, University of Innsbruck,
(4) Institute of Analytical Chemistry and Radiochemistry, University of Innsbruck,
(*) Corresponding Author


Poly(styrene/divinylbenzene) (PS/DVB) monolithic disk was prepared by in situ free-radical copolymerization of styrene and divinylbenzene in the presence of decanol and tetrahydrofuran as porogens. PS/DVB monolithic disks were produced in two different lengths 1.5 mm and 3 mm. The disks were used in reversed phase chromatography of proteins with 0.2 % trifuoroacetic acid (TFA) and 0.2 % TFA in acetonitrile as mobile phase A and B, respectively. The effect of gradient rate, flow rate, temperature and disk length on the separation of proteins were also studied. PS/DVB monolithic disks allow the rapid separation of proteins in reversed phase chromatography.


monolithic disk; poly(styrene/divinylbenzene); proteins

Full Text:

Full Text PDF


[1]   Barut, M., Podgornik, A., Merhar, M., and Strancar, A., 2003, J.Chromatogr.Lib., 67, 51

[2]   Chen, H., and Horváth, C., 1995, J. Chromatogr. A., 705, 3

[3]   Iberer, G., Hahn, R., and Jungbauer, A., 1999, LC.GC, 17, 998

[4]   Hearn, M.T.W., 1991, HPLC of Proteins, Peptides and Polynucleotides, VCH Publisher, Inc

[5]   Strancar, A., Podgornik, A., Barut, M., and Necina, R., 2002, Adv. Biochem Eng/Biotechnol, 76, 49

[6]   Abou-Rebyeh, H., Körber, F., Schubert-Rehgberg, K., Reusch, J., and Josic, D., 1991, J. Chromatogr., 566, 341

[7]   Josic, D., Reusch, J., Löster, K., Baum, O., and Reutter, W., 1992, J. Chromatogr. A., 590, 59

[8]   Belenkii, B.G., Podkladenko, A.M., Kurenbin, O.I., Mal’tsev, V.G., Nasledov, D.G., and Trushin, S.A., 1993,  J. Chromatogr. A., 645, 1

[9]   Dubinina, N.I., Kurenbin O.I., and Tennikova, T.B., 1996, J. Chromatogr. A., 753, 217

[10] Tennikova T.B., and Freitag, R., 2000, J. High Resol. Chromatogr., 23, 27

[11] Burke, T.W.L., Mant, C.T., and Hodges, R.S., 1991, The Effect of Varying Flow-Rate, Gradient-Rate, and Detection Wavelength on Peptide Elution Profiles in Reversed-Phase Chromatography in High- Performance Liquid Chromatography of Peptides and Proteins (Mant C.T., and Hodges, R.S., edt.), CRC press, Boca Raton

[12] Schlabach, T.D., and Wilson, K.J., 1987, J. Chromatogr., 385, 65

[13] Meek, J.L., and Rossetti, Z.L., 1981, J. Chromatogr., 211, 15

[14] Guo, D., Mant, C.T., Taneja, A.K., and Hodges, R.S., 1986, J. Chromatogr., 359, 519

[15] Antia, F.D., and Horvárth, C., 1988, J. Chromatogr., 435, 1

[16] Snyder, L.R., Stadalius, M., and Quarry, M.A., 1983, Anal. Chem., 55, 1412A

[17] Moore, R.M., and Walters, R.R., 1984, J.Chromatogr., 317, 119


Article Metrics

Abstract views : 334 | views : 354

Copyright (c) 2010 Indonesian Journal of Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.


Indonesian Journal of Chemistry (ISSN 1411-9420 / 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

Analytics View The Statistics of Indones. J. Chem.