A THERMOPHILIC MICROBE PRODUCING DEXTRANASE FROM HEATED SUGAR CANE

https://doi.org/10.22146/ijc.21794

Afaf Baktir(1*), Zumrotul Koiriyah(2), Ali Rohman(3)

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences University of Airlangga, Surabaya
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences University of Airlangga, Surabaya
(3) Department of Chemistry, Faculty of Mathematics and Natural Sciences University of Airlangga, Surabaya
(*) Corresponding Author

Abstract


A thermophilic aerobe microorganism designated NP4, was isolated from the heated sugar cane. It grew on dextran, and produced a thermoactive extracellular dextranase. Screening and isolation was done by assay of dextranase activity semi quantitatively on solid medium containing blue dextran. It provided several colonies with different morphology exhibited decolourized zones around, on culture plates containing blue dextran 2000R. The screening resulted in isolation of one microbe which efficiently assimilate dextran as carbon source. Dextranase production from the choised strain in liquid medium was conducted at room temperature for 8 hours with shaking speed of 125 rpm. The dextranase enzyme showed optimum pH of 8 and optimum temperature of 60 oC.


Keywords


thermophilic aerobe; sugar cane; dextranase activity

Full Text:

Full Text PDF


References

[1] Gale, A. and Inkerman, P.A., 1993, Int. Sugar J., 95 (1136), 309 – 313.

[2] Safarik, I., and Safarikovat, M., 1992, Biotech. Ap. Biochem., 16, 263 –268.

[3] El-Masry, H.G., 1991, Zentralbl Microbio., 146, 185 –192.

[4] Okushima, M., 1991, J. Genet., 66, 173 – 187.

[5] Baktir, A and Murdiyatmo, U., 2003, Jurnal Matematika dan Ilmu Pengetahuan Alam, 8, 45-50

[6] Murdiyatmo, U, Laksmi, A. Wiwik E.W. and Laksmi, H, 1994, Dekstranase bakterial : Produksi dan Penggunaannya di Pabrik Gula. Prosiding Seminar Hasil Penelitian dan Pengembangan Bioteknologi II, Cibinong.

[7] Nelson, N., 1944, J. Bio. Chem. 153: 375-381.

[8] Lestari, P., 1999, Eksplorasi Enzim Termostabil dari Mikrob Termofil, Hayati 7, 21-25

[9] Wynter, C, Patel, B.K.C, Bain, P, de Jersey, J., Hamilton, S., and Inkerman, P.A., 1996, FEMS Microb. Lett., 140, 271 –276.



DOI: https://doi.org/10.22146/ijc.21794

Article Metrics

Abstract views : 1621 | views : 2959


Copyright (c) 2010 Indonesian Journal of Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 

Indonesian Journal of Chemistry (ISSN 1411-9420 /e-ISSN 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

Web Analytics View The Statistics of Indones. J. Chem.