EVALUATION OF RE-USED HPTLC PLATE FOR QUALITATIVE AND QUANTITATIVE ANALYSIS

https://doi.org/10.22146/ijc.21743

Lestyo Wulandari(1*)

(1) Pharmacy Study Program, University of Jember, Jl. Kalimantan I/2 Jember 68121
(*) Corresponding Author

Abstract


The eficiency chromatography of HPTLC plate is better than conventional TLC plate but it more expensive. Due to the higher price of HPTLC plate compare to conventional TLC plate, the application of ";re-used"; HPTLC plate has been tested. After being used for analyzing samples, the plates were developed again with the mobile phase, then dried for 60 min before using for the next measurement. The qualitative chromatography profile showed that ";re-used"; HPTLC plate has less contaminant than new HPTLC plate and the RSD of the precision quantitative testing of the plates reused  three times showed a value > 2%.


Keywords


reused; HPTLC; TLC; quantitative; qualitative

Full Text:

Full Text Pdf


References

[1] Dienstrop, E.H., 2000, Applied Thin Layer Chromatography, Wiley-VCH Verlag GmbH, Weinheim

[2] Sherma, J. and Bernand, F., 2003, Handbook Of Thin Layer Chromatography, 3rd Ed, Easton, Pennsylvania, USA, Lafayette College

[3] Anonim, 2001, The United State Pharmacopoeia 25-National Formulary 20, Asia Ed; Rockville, United States Pharmacopoeial Convention

[4] Anonim, 2000, British Pharmacopoeia 2000, London, The Stationary Office

[5] Anonim, 1995, Farmakope Indonesia, Ed IV, Jakarta, Departemen Kesehatan Republik Indonesia

[6] Indrayanto, G., and Yuwono, M., 2002, Validation of TLC Analyses in Encyclophedia of Chromatography, New York, USA, Marcel Dekker

[7] Wulandari L, and Indrayanto G, 2003, J. Liq. Chromat. & Related Tech, 26, 2709-2717

[8] Anonim, 2003, Camag Bibliography Service, Cumulative CD Version 1.09, Camag Muttenz.



DOI: https://doi.org/10.22146/ijc.21743

Article Metrics

Abstract views : 1763 | views : 6568


Copyright (c) 2010 Indonesian Journal of Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 

Indonesian Journal of Chemistry (ISSN 1411-9420 /e-ISSN 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

Web Analytics View The Statistics of Indones. J. Chem.