FRACTIONATION OF FATTY ACID OMEGA 3, 6 AND 9 FROM SNAIL (Achatina fulica) USING COLOUM CHROMATOGRAPHY

https://doi.org/10.22146/ijc.21739

Winarto Haryadi(1*), Sugeng Triono(2)

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281
(*) Corresponding Author

Abstract


The extraction of fat from snail has been carried out by Soxhlet extractor with petroleum ether solvent. Fatty product from extraction was transesterificated in BF3/methanol solvent for an hour by reflux procedure, then extracted by n-hexane to produce methyl ester fatty acid. Free water fatty acid methyl ester was analyzed by Gas Chromatography-Mass spectrometer (GC-MS). Fatty acid ester was separated from its fractions use column chromatography in n-hexane, n-hexane:dietil eter (2:1 v/v), dietil eter, aseton, ethanol and methanol. This fractions wer also analized by GC-MS. From GC-MS data sheet can be obtained 5 fractions which details are; fraction 1 contains omega 3: 27.54 %, omega 6: 15.40 % and omega 9: 6.77 %. Fraction 2 contains omega 3: 3.08 %, omega 6: 15.62 % and omega 9: 10.72 %. Fraction 3 contains omega 6: 3.57 %, omega 9: 7.02 % and none omega 3 inside it. Omega 3, 6 and 9 can't be identification in fraction 4 and 5.

 

Keywords: extraction, transesterification, column chromatography, GC-MS

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DOI: https://doi.org/10.22146/ijc.21739

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Indonesian Journal of Chemisty (ISSN 1411-9420 / 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

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