POTENCY OF MUNG BEAN SPROUT AS ENZYME SOURCE (α-AMILASE)

Suarni Suarni; Rauf Patong

doi:10.22146/ijc.21679

POTENCY OF MUNG BEAN SPROUT AS ENZYME SOURCE (α-AMILASE)

https://doi.org/10.22146/ijc.21679

Suarni Suarni^(1*), Rauf Patong⁽²⁾

(1) Indonesian Cereals Research Institute. Jl. Dr. Ratulangi no.274, Maros 90514. PO.Box.1173 Makassar
(2) Department of Chemistry Faculty of Mathematics and Natural Science Hasanuddin University. Jl. Perintis Kemedekaan KM.10Makassar
(*) Corresponding Author

Abstract

Mung bean sprouts contain enzyme of α-amylase. A research on the effect of the sprout age and sprout varieties to the α-amylase activity and the protein level has been carried out in Laboratorium Bioproses BB Pascapanen Bogor using Full Factorial Random Design with two factorials (1) sprout age; 1, 2, 3, 4, and 5 days as well as (2) varieties of mung bean; Kenari, Bhakti and Parkit. Parameters observed were water and protein content of sprout, pH, activities of α-amylase, and dissolved protein in the enzyme extract. Results showed that the optimum temperature of α-amylase was 30 ºC, the highest protein level of sprout and the highest activity of α-amylase were given by the sprout of Bhakti at the age of three days. The water content in sprout was 65.23%, the protein level was 12.93 %, the dissolved protein in the enzyme extract was 2.88743 mg/mL, pH was 5.45, and the activity of enzyme was 4.09 Unit/mL. The potency of enzyme found in mung bean can be utilized in industries processing materials having high starch content, such as maize flour.

Keywords

sprout of mung bean; activity of α-amylase; protein

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DOI: https://doi.org/10.22146/ijc.21679