INHIBITION OF HUMAN LOW-DENSITY LIPOPROTEINS OXIDATION BY Hibiscus radiatus CUV. CALYCES EXTRACT

https://doi.org/10.22146/ijc.21655

Hernawan Hernawan(1*)

(1) Technical Implementation Unit for Development Chemical Engineering Processes, Indonesian Institute Of Sciences, Yogya-Wonosari Street 32nd Km, Gading, Playen, Gunungkidul, Yogyakarta, 55861
(*) Corresponding Author

Abstract


Hibiscus radiatus Cuv calyces extracts rich in polyphenols was screened for their potential to inhibit oxidation of human low-density lipoproteins-cholesterol (LDL-C) in vitro. The inhibition of LDL-C oxidation (antioxidant activity) was determined by measuring the formation of conjugated dienes and thiobarbituric acid reagent substances (TBARS). LDL-C oxidation was carried out in the presence of H. radiatus Cuv calyces extract (20 and 50 μM). CuSO4 (10 μM) was used as the oxidation initiator and  butylated hydroxytoluene (BHT) at 50 μM was used as standard antioxidant. The protective effect of H. radiatus Cuv. calyces extract toward human low-density lipoproteins, complex lipid system was  demonstrated by significant increase lag time (> 103 min), diminished of the propagation rate (44 %), and diminution of conjugated dienes formation 59.42 % (50 μM) compared to control.


Keywords


antioxidant; conjugated dienes; Hibiscus radiatus Cuv; low-density lipoproteins-cholesterol

Full Text:

Full Text Pdf


References

[1]   Shireqi, I., Reddy, P., Brenner, D.E., 2000, Crit. Rev. Oncol/Hematol, 33, 157-167

[2]   Finkel, T., and Holbrook, N.J., 2000, Nature, 408, 239-247

[3]   Pietta P.G., 2000, J. Nat. Prod., 63, 1035-1042

[4]   Frei, B., 1995, Crit. Rev. Food. Sci. Nitr:, 35, 83-98

[5]   Hernawan, and Angwar M., 2007, Study on In Vitro Antioxidant Effect of Hibiscus radiatus Cuv. Calyces Extract.  Proceeding of National Seminar on Sciences and Technology 2007, Lampung University

[6]   Taga, M.S., Miller E.E., Pratt, D.E., 1984, J. Am. Oil Chem. Soc., 61, 928-931

[7]   Sattler, W., Mohr, D., Stocker, R.,1994, Methods in Enzymol:, 233, 469-489

[8]   Esterbauer, H., Streigl, G., Puhl, H., Rothenender, M., 1989, Free. Radic. Ras. Commun., 6, 67-75

[9]   Duh, P.D., Tu, Y.Y., Yen, G.C., 1999, Lebenmittel-Wissenschaft und Technogie., 32, 269-277

[10] Roginsky, V., 2003, Arch Biochem Biophys., 414, 261-270



DOI: https://doi.org/10.22146/ijc.21655

Article Metrics

Abstract views : 375 | views : 338


Copyright (c) 2010 Indonesian Journal of Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 


Indonesian Journal of Chemistry (ISSN 1411-9420 / 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

Web
Analytics View The Statistics of Indones. J. Chem.