Supartono Supartono(1*), Nanik Wijayati(2), Lina Herlina(3), Enny Ratnaningsih(4)

(1) Chemistry Department, Gd. D6-2nd floor, FMIPA UNNES Sekaran Gunungpati Semarang, 50229
(2) Chemistry Department, Gd. D6-2nd floor, FMIPA UNNES Sekaran Gunungpati Semarang, 50229
(3) Biology Department, Gd D6-1st floor, FMIPA UNNES Sekaran Gunungpati Semarang, 50229
(4) Chemistry Department, FMIPA ITB, Jl. Ganesha No. 10 Bandung, 40132
(*) Corresponding Author


The efforts to get a new antibiotic require to be done continuously, because infection diseases still become the main health problems in Indonesia. A new local strain of Bacillus subtilis BAC4 has been known producing an antibiotic that inhibites Serratia marcescens ATCC 27117 growth. Nevertheless, the optimum conditions have not been studied seriously. The objective of this research was to conduct mutation on B. subtilis BAC4 in order to obtain a mutant cell that overproduct in producing antibiotic. The mutation process was performed by using acridine orange of 1 g.L-1 randomly at various volumes. The production of antibiotic was conducted using batch fermentation and antibiotic assay was performed with agar absorption method using S.  marcescens ATCC 27117 as bacteria assay. Research result provided a B. subtilis M10 mutant with overproduction of antibiotic. Characterization of B. subtilis M10 mutant showed that the mutant cell has size of (0.5-1.0) µm x (1.85-2.5) µm; spore has the form of ellipse with thick wavy wall, positive reaction for catalase, and forming acid from glucose and xylose.


mutant; Bacillus; acridin; antibiotics

Full Text:

Full Text Pdf


[1]   Petersen, P.J., Bradford, P.A., Weiss, W.J., Murphy, T.M., Sum P.E., and Projan, S.J., 2002, Antimicrob. Agents Chemother., 46, 8, 2595-2601.

[2]   Tsuji, M., Takema, M., Miwa, H., Shimada, J., and Kuwahara, S., 2003, Antimicrob. Agents Chemother., 47, 8, 2507-2512.

[3]   Griffith, D.C., Harford, L., Williams, R., Lee, V.J., and Dudley, M.N., 2003, Antimicrob. Agents Chemother., 47, 1, 43-47.

[4]   Navarro, P.G., Osso, B.Q., Ortiz, R.G., Parras, P.J., M.d.l., Puentedura, M.I.M., and Gonzalez, M.C.C., 2004, Antimicrob. Agents Chemother., 48, 3, 1058-1060.

[5]   Tsukiyama, R.I., Katsura, H.,  Tokuriki, N., and Kobayashi, M., 2002, Antimicrob. Agents Chemother., 46, 5, 1226-1230.

[6]   Gezer, K., Duru, M.E., Kivrak, I., Turkoglu, A.,  Mercan, N., Turkoglu, H., and Gulcan, S., 2006, Afr. J. Biotechnol., 5, 20, 1924-1928.

[7]   Karthikumar, S., Vigneswari, K., and Jegatheesan, K., 2007, Sci. Res. Essays, 2,4, 101-104.

[8]   Lowenberger, C., Charlet, M., Vizioli, J., Kamal, S., Richman, A., Christensen, B.M.,  and Bullet, P., 1999, J. Biol. Chem., 274, 29, 20092-20097.

[9]   Lugardon, K., Raffner, R., Goumon, Y., Corti, A., Delmas, A., Bulet, P., Aunis, D., and Boutique, M., 2000, J. Biol. Chem., 275, 15, 10745-10753.

[10] Azevedo, E.C., Rios, E.M., Fukushima, K., and Takaki, G.M.C., 1993, Appl. Biochem. Biotechnol., 42, 1-7.

[11] Phillips, I., 1999, J. Antimicrob. Chemother., 44, 6, 725-728.

[12] Bell, R.G., 1991, J. Pharm. Biomed. Anal., 9, 10, 843-847.

[13] Milner, J., Silo-Suh, L., Lee, J.C., He, H., Clardy J., and Handelsman, J., 1996, Appl. Environ. Microbiol., 62, 8, 3061-3065.

[14] Whistler, C., Stockwell, V.O., and Loper, J.E., 2000, Appl. Environ. Microbiol., 66, 7, 2718-2725.

[15] Tamehiro, N., Hosoya, Y.O., Okamoto, S., Ubukata, M., Hamada, M., Naganawa, H., and Ochi, K., 2002, Antimicrob. Agents Chemother., 46, 2, 315-320.

[16] Bernal, G., Illanes, A., and Ciampi, L., 2002, EJB., 5, 1, 12-20.

[17] Moeis, R.M., Ratnaningsih, E., Susanto, A.H., and Liang, O.B., 2000, A New Bacillus Strain Producing Penicillin Acylase, The forth ITB-UKM Joint Seminar on Chemistry, Yogyakarta, 75-81.

[18] Kusumaningrum, H.P., 1999, Determinasi Spesies Bacillus sp. BAC 4 Secara Mikrobiologis dan Molekuler, Tesis Magister, Program Pascasarjana ITB Bandung, 41-66.

[19] Meevootisom, V., Somsuk, P., Prachartam, R., and Feegel, L.W., 1983, Environ. Microb., 46, 1227-1229.

[20] Butcher, R.A., Schroeder, F.C., Fischbach, M.A., Straight, P.D., Kolter, R., Walsh, C.T., and Clardy, J. 2007, PNAS., 104, 5, 1506-1509.

[21] Crueger, W., and Crueger, A., 1984, Biotechnology: A Tectbook of Industrial Microbiology, Sinauer Associates, Inc., Sunderland-USA.

[22] Supartono, Wijayati, N., Herlina L., and Ratnaningsih, E., 2006, Produksi dan Karakterisasi Antibiotika dari Bacillus subtilis BAC4 Galur Lokal Baru, Laporan Penelitian Hibah Bersaing, DP2M Dirjen Dikti Depdiknas RI.Tan, K.  1998


Article Metrics

Abstract views : 356 | views : 634

Copyright (c) 2010 Indonesian Journal of Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.


Indonesian Journal of Chemistry (ISSN 1411-9420 / 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

Analytics View The Statistics of Indones. J. Chem.