PHOTODEGRADATION AND ANTIOXIDANT ACTIVITY OF CHLOROPHYLL a FROM SPIRULINA (Spirulina sp.) POWDER

https://doi.org/10.22146/ijc.21628

Rebecca Christiana(1), Hari Kristopo(2), Leenawaty Limantara(3*)

(1) Magister Biology, Satya Wacana Christian University, Jl. Diponegoro 52-60, Salatiga 50711
(2) Ma Chung Research Center, Universitas Ma Chung, Villa Puncak Tidar N-01, Malang 65151
(3) Magister Biology, Satya Wacana Christian University, Jl. Diponegoro 52-60, Salatiga 50711; Ma Chung Research Center, Universitas Ma Chung, Villa Puncak Tidar N-01, Malang 65151
(*) Corresponding Author

Abstract


Research on chlorophyll a from Spirulina (Spirulina sp.) powder has been done in order to determine chlorophyll a content, to compare degradation pattern and kinetics degradation of chlorophyll a and crude extract as well as to investigate the difference of antioxidant activity of chlorophyll a with or without irradiation. Chlorophyll a content in spirulina powder was calculated by using Porra's equation. Irradiation and recovery ability of chlorophyll a were measured by using volpi light (Intralux 6100) equipped with daylight filter. The antioxidant activity of chlorophyll a was determined by using DPPH method. The results showed that chlorophyll a content was 2.886 μg/g dry weight. Chlorophyll a has the higher stability than crude extract against 60 minutes irradiation at room temperature. Kinetics degradation of chlorophyll a was based on second order while crude extract based on first order. Chlorophyll a and crude extract have recovery ability. The results also showed that antioxidant activity of chlorophyll a was increased after 60 minutes of irradiation.


Keywords


antioxidant activity; chlorophyll a; photodegradation; spirulina powder

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References

[1] Jensen, S. and Knutsen, G., 1993, J. Appl. Phycology, 5, 495-504.

[2] Kozlenko, R. and Henson, R. H., 1998, Healthy & Nat. J., 3, 5.

[3] Manoj, G., Venkataraman, L. V. dan Srinivas, L., 1992, In : Seshadri and Bai. Spirulina. MCRC, 48-154.

[4] Miranda, M. S., Cintra, R. G., Barros, S. M. and Mancini-Filho, J., 1998, Braz. J. Med. Biol. Res, 31, 1057-1079.

[5] Moore, A., 2001, EMBO Reports, European Molecular Biology Organization. 21, no. 61.

[6] Torres-Duran, P. V., Miranda-Zamora, R., Paredes-Carbajal, M. C., Mascher, D., Castillo, B., Diaz-Zagoya, J. C. and Juarez-Oropeza, M. A., 1999, J. Ethnopharmac., 64, 141-147.

[7] Zhi-gang, Z., Zhi-li, L., and Xue-xian, L., 1997, Acta. Botanica Sinica, 39, 77-81.

[8] Brotosudarmo, T. H. P. dan Limantara, L., 2002, http://www.kompas.com/kompas-cetak/0210/29/ iptek/klor30.htm

[9] Buratti, S., Pallegrini, N., Brenna, O. V. and Mannino, S., 2001, J. Agric. Food Chem, 49, 5136-5141.

[10] Limantara, L., 2004, Prosiding Seminar Nasional Aplikasi Sains dan Matematika dalam Industri, hal. 153-168.UKSW.

[11] Okai, Y. and Higashi-Okai, K., 2001, J. Food Sci., 81(15), 1443-1446.

[12] Nakamura, Y., Murakami, A., Koshimizu, K. dan Oshigashi, H., 1996, Canc. Letters, 108, 247-255.

[13] Lanfer-Marquez, U. M., Barros, R. M. C. and Sinnecker, P., 2005, J. Food Res. Int., 38, 885-891.

[14] Gross, J., 1991, Pigment in Vegetables, Chlorophyll and Carotenoids, Van Nostrand Reinhold, New York

[15] Porra, R. J., 1991, Recent Advances and Re-assessment in Chlorophyll Extraction and Assay Procedures for Terrestrial, Aquatic, and Marine Organism, Including Recalcitrant Algae, CRC Press, London.

[16] Rowan, K. S., 1989, Photosynthetic Pigments of Algae, Cambridge University Press, Cambridge.

[17] Wang, B., Yu, Z. R. and Hwang, L. S., 1995, J. Chinese Agricul. Chem. Soc., 33(5), 550-560.

[18] Limantara, L., Koehler, P., Wilhelm, B., Porra, R.J. and Sheer, H., 2006, Photochem. Photobiol, 82, 770-780.

[19] Lee, J. H., Park, J. H., and Choi, J. S., 1996, in : Amin, I., Norazaidah, Y., dan Hainida, K. I. E., 2006, Journal Food Chem., 94, 47-52.

[20] Kabinawa, I. N. K., 2006, Spirulina Ganggang Penggempur Aneka Penyakit, PT. AgroMedia Pustaka, Jakarta,.

[21] Craig, I. W. and Carr, G., 1968, Biochem. J, 106, 361.

[22] El-Baky, H. H. A., 2003, J. Med. Sci., 3(4), 314-324.

[23] Anonim, 1997, IUPAC Compendium of Chemical Terminology, Second edition.

[24] Endo, Y., Usuki, R., and Kaneda, T., 1984, Agricultural Biol.Chem, 48 (4), 985-989.

[25] Merzlyak, M. N., Pogosyan, S. I., Lekhimena, L., Zhigalova, T. V., Khozina, I. F., Cohen, Z., and Khruschev, S. S., 1996, Russian J. Plant Physiology, 43, 2, 160-168.

[26] Fiedor, J., Fiedor, L., Kammhuber, N., Scherz, A., and Scheer, H., 2002, Photochem. Photobiol., 76 (2), 145-152.

[27] Douglas, B. M., 2002, Colour in Food, CRC Press, Cambridge.

[28] Matile, P., Hörtensteiner, S., and Thomas, H., 1999, Annu. Rev. Plant Physiol. Plant Mol. Biol., 50, 67-95.

[29] Feruzzi, M. G. and Blakeslee, J., 2007, Nutrition Res., 27, 1-12.



DOI: https://doi.org/10.22146/ijc.21628

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