DETERMINATION AND VALIDATION OF MEBHYDROLINE NAPADISYLATE IN TABLETS BY HPLC

https://doi.org/10.22146/ijc.21610

Lestyo Wulandari(1*)

(1) Department of Chemical Pharmacy, School of Pharmacy, Jember University, Jl. Kalimantan I/2, Jember, 68121
(*) Corresponding Author

Abstract


An accurate and sensitive HPLC method has been developed for the determination of mebhydroline napadisylate in the tablet. The Chromatography was performed on a reversed phase C-18 column, using a mobile phase of acetonitrile : ammonia 25% (80 : 20 v/v) at ambient temperature 25±5 °C and UV detection operates at 320 nm in an overall analysis time of about 15 min, based on peak area. This HPLC method is selective, precise, and accurate and can be used for routine analysis of pharmaceutical preparation in industrial quality-control laboratories.


Keywords


HPLC; mebhydroline napadisylate; validation

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References

[1] ---, 2006, ISO (Informasi Spesialite Obat) Indonesia,Volume XXXV, Ikatan Sarjana Farmasi Indonesia, Jakarta, Indonesia, 123, 126,127, 129, 132, 133

[2] Pharmaceutical Press, 2004, Clarke’s Analysis of Drugs Poisons. 3rd ed.. Vol. 1. London. 517, 523

[3] Pharmaceutical Press, 2004, Clarke’s Analysis of Drugs Poisons. 3rd ed.. Vol. 2. London. 1203

[4] Funk, W., Damman, V., Donnervert, G., 1992; Qualitassicherung in der Analytischen Chemie; VCH; Weinheim, New York, Basel, Cambrige, 1-36, 161-180.

[5] Hahn-Dienstrop, E. 2000, Applied Thin Layer Chromatography; Wiley-VCH Verlag GmbH; Weinheim, 198-205

[6] Huber, L., 2007, Validation and Qualification in Analytical Laboratories. 2nd ed. New York, 140-150

[7] Renger, B., Jehle, H., Fischer, M., and Funk, W. 1995, J. Planar Chromatogr.-Mod TLC, 8, 269-278

[8] Carr, G.P. and Wahlich, J.C. 1990, J. Pharm. Biomed. Anal. 8, 613-618

[9] Ermer, J. 2001, J. Pharm. Biomed. Anal. 24, 755-767

[10] Indrayanto, G and Yuwono, M. 2003, Validation of TLC Analyses. Encyclopedia of Chromatography. New York: Marcel Dekker, Inc. 1-3



DOI: https://doi.org/10.22146/ijc.21610

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Indonesian Journal of Chemistry (ISSN 1411-9420 /e-ISSN 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

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