Tutik Murniasih(1*), Kyoko Adachi(2)

(1) Research Center for Oceanography Indonesian Institute of Sciences Jl. Pasir putih No. 1 Ancol Timur Jakarta 14430
(2) Marine Biotechnology Institute Co. Ltd., Kamaishi Lab.,3-75-1 Heita, Iwate 026-0001, Kamaishi City
(*) Corresponding Author


In our course of screening for secondary metabolite derived from marine bacterial, we isolate the antimicrobial compound collysmicin A from the ethyl acetate extract of Streptomyces sp Q-629K. Separation of this compound was carried out by silica gel open column chromatography. Purification of an active compound was done using HPLC C18 with acetonitril-water system. Determination of chemical structure was done by 1H, 13C NMR and LC-MS analysis. Collysmicin A was contained in fraction 3, fraction 7.2 and fraction 8.7. The antimicrobial assayed from purified compound Fr.8.7 gave diameter inhibition approximately 13 mm against S. aureus and 12 mm against B. subtilis.


antimicrobial; collismycin A; marine Streptomyces sp

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[1] Berdy, J., 1982, Search and Discovery Methods for Novel Antimicrobials in: Bioactive Metabolites from Microorganisms (M.E. Bushell and U. Grafe, eds), Elsevier, Amsterdam, pp. 3-25.

[2] Beetina, V., 1983, The Chemistry and Biology of Antibiotics, Elsevier, Amsterdam.

[3] Fusetani, N., 2000, Drugs from The Sea. Karger, Basel Switzerland, pp.1-5

[4] Burkholder, P.R., Pfister, R.M., and Leitz, F.P., 1966, Appl. Microbiol. 15:85-89.

[5] Kitahara, T., Naganawa, H., Okazaki, T., Okami, Y., and Umezawa, H., 1975, J. Antibiot. 28:280.

[6] Okazaki, T., Kitahara, T., and Okami, Y., 1975, J. Antibiot. 28:176-184.

[7] Hotta, K., Yoshida, T., Hamada, M., and Okami, Y., 1980, J. Antibiot. 33:1515.

[8] Okami, Y., Okazaki,T., Kitahara, T., and Umezawa, H., 1976, J. Antibiot. 29:1019-1025.

[9] Sato, K., Okazaki, T., Maeda, K., and Okami, Y., 1978, J. Antibiot. 31:632

[10] Shindo, K., Yamagishi, Y., Okada, Y., Kawai, H., 1994, J. Antibiot. vol.47 no.9. 1072-1074.

[11] Tsuge, N., Furihata, K., Shin-Ya, K., Seto, H., 1999, J. Antibiot. vol. 52, No.5 pp105-107.

[12] Gomi, S., Ito, S., Azito, K., Kariya, Y., and Koyama, M. (Meiji Seika Kaisya, Ltd.), (1993) A New Microbial and Antitumor Agent SF2738. Jpn. Kokai 78322 (’93).


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Indonesian Journal of Chemistry (ISSN 1411-9420 /e-ISSN 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

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