THE EFFECT OF PROCESS CONDITIONS IN PREPARATION OF VEGETABLE BROTH AS SAVORY FLAVOR FROM MUNG BEANS (Phaseolus radiatus L.) USING INOCULUM OF Rhizopus-C1

https://doi.org/10.22146/ijc.21592

Agustine Susilowati(1*), Aspiyanto Aspiyanto(2), Hakiki Melanie(3), Yati Maryati(4)

(1) Research Centre for Chemistry, Indonesian Institute of Sciences Kawasan PUSPIPTEK, Serpong, Tangerang - 15314
(2) Research Centre for Chemistry, Indonesian Institute of Sciences Kawasan PUSPIPTEK, Serpong, Tangerang - 15314
(3) Research Centre for Chemistry, Indonesian Institute of Sciences Kawasan PUSPIPTEK, Serpong, Tangerang - 15314
(4) Research Centre for Chemistry, Indonesian Institute of Sciences Kawasan PUSPIPTEK, Serpong, Tangerang - 15314
(*) Corresponding Author

Abstract


Preparation of vegetable broth from mung beans (Phaseolus radiatus L.) in semi pilot scale is an attempt development to get product of savory flavor in larger scale. The aim of this activity was to find out the effect of process multiplication on composition of vegetable broth from mung beans using inoculum of Rhizopus-C1 through brine fermentation in mixtures of inoculum, salt, and mung beans of 26, 23, and 51 %. This activity was conducted in both temperature of fermentation (room temperature and 30 °C), various time of fermentation (0, 2, 4, 6, 8, 10, and 12 weeks) and process scales, namely laboratory scale (300 g) and semi pilot scale (± 25 kg), respectively. The result of experiment indicated that fermentation temperature and time and process scales were tend to affect on composition of product. The length of fermentation time would increase concentrations of dissolved protein, N-amino and reducing sugar, decreased concentrations of fat and Volatile Reduction Substance (VRS), while concentrations of total protein, and water were tend to be constant in laboratory and semi pilot scales at the both process temperatures. Multiplication in preparation process of inoculum (7 kg) using starter of Rhizopus-C1 resulted inoculum with activities of proteolytic of 0.71 U/g, and amilolytic of 17.5 U/g at 56 h of incubation. The whole process in semi pilot scale decreased composition of products. The optimal treatment based on recovery of total protein, and the highest amino acids as N-amino in semi pilot scale was at fermentation temperature of 30 ºC for 10 weeks with concentrations of water of 44.96%, total protein of 11.77% (dry matter), dissolved protein of 8 mg/mL, N-amino of 15.4 mg/mL, reducing sugar of 582.5 mg/mL, fat of 0.26% and, VRS of 90 µeq.reduction/g.


Keywords


Brine fermentation; vegetable broth; mung beans (Phaseolus radiatus L.); Rhizopus-C1; semi pilot

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References

[1] Haseltine, C. W. and Wang, H. L., 1980, Fermented Soybean Food Product, AVI Publishing Company Inc., Westport, Connecticut.

[2] Susilowati, A., and Aspiyanto, 2005, Pengaruh Aktivitas Proteolitik Inokulum Rhizophus sp-PL19 dan Aspergillus sp-K3 Terhadap Komposisi Protein Kaldu Nabati Dari Kacang Hijau (Phaseolus radiatus L.), Prosiding Seminar Nasional Teknologi Inovasi Pascapanen, Balitbang Pascapanen Pertanian, Departemen Pertanian – Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Bogor, ISBN 979-97379-8-2.

[3] Beuchat, L. R., 1987, Fermented Food of Orient, in Biotechnology, Rehm H.J. and Reed G. (eds), Vol. 5, Weinheim.

[4] Susilowati, A., and Aspiyanto, 2005, Potensi Kacang Merah (Phaseolus vulgaris L.) Sebagai Substrat Inokulum Dengan Starter Rhizophus sp-C1 dan Aspergillus sp-K1, Prosiding Seminar Nasional, Jaringan Kerjasama Kimia Indonesia, Yogyakarta, ISSN 0854-4778.

[5] Iskandar, Y. M., Raharti, S. P., and Budiwati, T. A., 2002, Pembuatan Inokulum Menggunakan Isolat Rhizopus-C1 dan Rhizopus-C2 Pada Substrat Campuran, Prosiding Seminar Nasional Tantangan Penelitian Kimia Dalam Era Biologi dan Super Informasi, Pusat Penelitian Kimia - LIPI, Jakarta, ISBN 979-9477-69-7.

[6] Damarjati, J., 2000, Potensi dan Pendayagunaan Sumberdaya Bahan Pangan Loka Serealia, Umbi-umbian dan Kacang-kacangan, Lokakarya Pengembangan Pangan Alternatif, KMRT, HKTI & BPPT, Jakarta.

[7] Husaini, 2000, Optimasi Pendayagunaan Komoditas Pangan yang Kurang Termanfaatkan, Lokakarya Pengembangan Pangan Alternatif, KMRT, HKTI & BPPT, Jakarta.

[8] Winarno, F. G., 2002, Flavor Bagi Industri Pangan, Bogor, M-Brio Press-ISBN 979-3098-SI-1.

[9] Danielle, R. R., Tanaka, T., and McDaniel, A., 2006, J. Physology & Behav 88, 215 – 226.

[10] Martin, R. Y., Natalie, J. G., Sirous, M., and John, P., 2008, J. Physology & Behav, 93, 958 – 966.

[11] Nagodawithana, T., 1993, Enzymes Associate with Savory Flavor Enhancement, in Enzymes in Food Processing, Tilak Nagodawithana & Gerald Reed, Academic Press Inc., San Diego, California, 92101-4311.

[12] Wood B. J. B., 1982, Soy Sauce and Miso, in Rose A. H., Fermented Food, Volume 1, Academic Press, Inc., New York.

[13] Steinkraus, K. H., Cullen, R. E., Pederson, C. S., and Nellis, L. F., 1983, Hand Book of Indigenous Fermented Foods, Marcell Dekker, New York dan Basel.

[14] Nagodawithana, T. W., Savory Flavors in Alan G., Bioprocess, Fragrance, and Color Ingredients, John Wiley & Sons, Inc., New York, 1994.

[15] A.O.A.C., 1995, Official Method of Analysis of Association of Official Analytical Chemistry, Washington D.C.

[16] Asikin, Z., 1986, Penuntun Belajar Kimia: Teori dan Soal, Penerbit Wijaya, Jakarta.



DOI: https://doi.org/10.22146/ijc.21592

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