STUDY ON THE SYNTHESIS OF MONOLAURIN AS ANTIBACTERIAL AGENT AGAINTS Staphylococcus aureus

Galuh Widiyarti; Muhammad Hanafi; Wahyudi Priyono Soewarso

doi:10.22146/ijc.21569

STUDY ON THE SYNTHESIS OF MONOLAURIN AS ANTIBACTERIAL AGENT AGAINTS Staphylococcus aureus

https://doi.org/10.22146/ijc.21569

Galuh Widiyarti^(1*), Muhammad Hanafi⁽²⁾, Wahyudi Priyono Soewarso⁽³⁾

(1) Research Center for Chemistry-Indonesian Institut of Science Kawasan PUSPIPTEK Serpong, Tangerang 15314
(2) Research Center for Chemistry-Indonesian Institut of Science Kawasan PUSPIPTEK Serpong, Tangerang 15314
(3) Departement of Chemistry, Mathematics and Science Faculty, Indonesian University, Depok
(*) Corresponding Author

Abstract

The monolaurin compound had been synthesized from lauric acid and glycerol by using sulfuric acid (H₂SO₄) as catalyst. The synthesis of monolaurin was done by batch esterification on the free solvent system. The esterification reaction was performed on the equivalent mol ratio between lauric acid and glycerol 1:1, in the presence of 5% H₂SO₄, at 130 °C, for 6 hours, produced ester compounds on 59.29%. The products of column chromatography on silica gel purification are monolaurin and dilaurin in amount of 31.05 and 4.48%, respectively. The monolaurin and dilaurin were identified by TLC, FTIR, GC-MS, and NMR spectrometer. The spectral data of monolaurin was compared to spectral data of standard monolaurin. The result of NMR identifications showed that synthesis products were a-monolaurin and a,a'-dilaurin. The antibacterial activity of synthesis products was tested against Staphylococcus aureus. The activity result showed that the antibacterial activity of monolaurin is more active than dilaurin.

Keywords

monolaurin; esterification; identification; antibacterial activity

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