Abdul Rahman(1*), Amri Ismail(2), Desi Jumbianti(3), Stella Magdalena(4), Hanggara Sudrajat(5)

(1) Department of Mechanical Engineering, Malikussaleh University, Jl. Tgk. Chick Ditiro No. 26 Lancang Garam, Po.Box.141, Lhokseumawe 24351, Nanggroe Aceh Darussalam, Indonesia
(2) Department of Chemical Engineering, Malikussaleh University, Jl. Tgk. Chick Ditiro No. 26 Lancang Garam, Po.Box.141, Lhokseumawe 24351, Nanggroe Aceh Darussalam, Indonesia
(3) Biomolecular Engineering Research Institute, 6-2-3, Furuedai, Suita 565-0874, Japan
(4) Biomolecular Engineering Research Institute, 6-2-3, Furuedai, Suita 565-0874, Japan
(5) Faculty of Technobiology, Atma Jaya Catholic University of Indonesia, Jl. Jenderal Sudirman 51, Jakarta 12930, Indonesia
(*) Corresponding Author


In this paper, we report a suitable method for extracellular synthesis of copper oxide nano particles by using Phormidium cyanobacterium. We hypothesize that synthesis of copper oxide nano particles is believed to occur by extracellular hydrolysis of the cationic copper by certain metal chelating anionic proteins/reductase secreted by bacteria under simple experimental conditions like aerobic environment, neutral pH and room temperature. Proteins not only reduce Cu (II) into copper oxide nano particles (CONPs) but also plays significant role in stabilization of formed nanoparticles at room temperature. Further TEM, SEM, XRD and FTIR analysis have confirmed the synthesis of nano particles through microbial route. Extracellular induction of metal chelating proteins/reductase was analyzed by SDS-PAGE.


Synthesis; copper oxide nano particles; Phormidium cyanobacterium

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