Yohanes Buang(1*)

(1) Laboratory of Applied Biochemistry, Department of Applied Biological Science, Saga University, Saga Shi, Honjo Machi-1, Saga, Japan; Department of Chemistry, Faculty of Science and Engineering, Nusa Cendana University, Jln. Adisucipto Penfui-Kupang
(*) Corresponding Author


The effects of dietary adenine on growth, lipid secretion, and liver lipid contents were studied. Rats were paired-fed 0.25 % adenine diet or a diet without adenine for 10 days. The final body weight and relative liver weight were recorded. Serum lipid profiles were measured using enzyme assay kits. Lipids of liver tissues were extracted and the lipid contents were determined. A peach of liver was prepared to determine the activities of fatty acid synthase (FAS) and fatty acid β-oxidation. The results showed that animals fed an adenine diet reduced slightly body weight and relative liver weight and promoted serum triglyceride (TG), phospholipids (PL), total cholesterol, and high density lipoprotein concentrations. Their promotions were approximately by 74%, 20%, 27%, and 27%, respectively. The liver PL content increased significantly whereas the promotions of liver TG and cholesterol failed to reach significant level. Furthermore, the FAS activity slightly stimulated, but fatty acid β-oxidation significantly decreased. In conclusion: dietary adenine suppressed slightly growth of body weight and relative liver weight but promoted secretion of lipid compounds from liver tissue into extra-hepatic and liver PL content. The enzyme activity of lipogenic was slightly stimulated whereas the lipolytic was significantly inhibited.


adenine; serum lipid; triglyceride; high density lipoprotein; cholesterol

Full Text:

Full Text PDF


[1]   Lehninger, Nelson, and Cox, 1993, Principles of Biochemistry, 2nd ed., Worth Publishers, Inc. USA.

[2]   Stipanuk, M.H., 2006, Biochemical, Physiological, and Molecular Aspects of Human Nutrition, 2nd ed.,, Published by Saunders-Elsevier, New York, 666-672.

[3]   Allen, L., deBenoist, B., and Dary, O., 2006, Guidelines on food fortification with micronutrients, WHO Library Cataloguing-in-Publication Data, 73-80.

[4]   Website http://ghr.nlm.nih.gov/ghr/glossary/adenine. Accessed July 10, 2009.

[5]   Burnstock, G., 2007, Cell. Mol. Life Sci., 64, 12, 1471-1483.

[6]   Chan, E.S.L., Montesinos, M.C., Fernandez, P., Desai, A., Delano, D.L., Yee, H., Reiss, A.B., Pillinger, M.H., Chen, J-F., Schwarzschild, M.A., Friedman, S.L., and Cronstein, B.N., 2009, Br. J. Pharmacol., 148, 8, 1144-1155.

[7]   Vallon, V., Hlbauer, B.M., and Oswald, H., 2006, Physiol. Rev., 86, 901-940.

[8]   Zimmermann, H., 2000, Naunyn-Schmiedeberg's Arch. Pharmacol., 362, 299-309.

[9]   Horiuchi, T., Dietrich, H.H., Tsugane, S., and Dacey Jr., R.G., 2001, Am. J. Physiol. Heart Circ. Physiol., 280, 767-776.

[10] Sairenchi, T., Iso, H., Irie, F., Fukasawa, N., Yamagishi, K., Kanashiki, M., Saito, Y., Ota, H., and Nose, T., 2005, Hypertens. Res., 28, 901-909.

[11] Aller, M.A., Vara, E., García, C., Nava, M.P., Angulo, A., Sánchez-Patán, F., Calderón, A., Vergara, P., and Jaime, A., 2006, World J. Gastroenterol., 12, 42, 6828-6834.

[12] Sánchez-Patán, F., Anchuelo, R., Aller, M.A., Vara, E., García, C., Nava, M.P., and Arias, J., 2008, Lipids in Health and Disease, 7, 4, 1-10.

[13] Blanco-Vaca, F., Escolà-Gil, J.C., Martín-Campos, J.M., and Julve, J., 2001, J. Lipid Res., 42, 1727-1739.

[14] Ohashi, R., Mu, H., Wang, X., Yao, Q., and Chen, C., 2005, Oxf. J. Med., 98, 12, 845-856.

[15] Cohn, J.S., Wat, E., Kamili, A., and Tandy, S., 2008, Curr. Opin. Lipidol., 19, 257-262.

[16] Ralevic, V., and Burnstock, Y., 1998, Am. Soc. Pharmacol. Exp. Ther., 50, 3, 413-492.

[17] Julve, J.C. Escola-Gil, J.C., Rotllan, N., Fievet,C., Vallez, E., de la Torre, C. , Ribas, V., Sloan, J.H., and Blanco-Vaca, F., 2010, Arterioscler. Thromb. Vasc. Biol., 30, 2, 232-238.

[18] Hime, N.J., Drew, K.J., Wee, K., Barter, P.J., and Rye, K.A., 2006, J. Lipid Res., 47, 1, 115-122.

[19] Tanaka, T., Doi, K., Mamiya, R.M., Negishi, K., Portilla, D., Sugaya, T., Fujita, T., and Noiri, E., 2009, Am. J. Pathol., 174, 4, 1203-1211.

[20] Chiu, C.H., Lu, T.Y., Tseng Y-Y., and Tzu-Ming Pan, T-M., 2006, Appl. Microbiol. Biotechnol., 71, 2, 238-245.

[21] Kit, Y.Y., Semenov, D.V., Kuligina, E.V., and Richter, V.A., 2000, Biochemistry (Mosc.). 65, 2, 237-43.

[22] Adachi, Y., Sasagawa, I., Tateno, T., Tomaru, M., Kubota, Y., and Nakada T., 2009, Endocrinol. Diabetes, 30, 2, 115-118.

[23] Siahanidou, T., Mandyla, H., Papassotiriou, I., and Anagnostakis, D., 2004, J. Pediatr. Gastroenterol. Nutr., 38, 56–60.

[24] Folch, J., Lees, M., and Sloane-Starley, G.H., 1957, J. Biol. Chem., 226, 497-509.

[25] Fletcher, M.M., 1968, Clin. Chim. Acta, 22, 393-7.

[26] Barlett, G.R., 1959, J. Biol. Chem., 234, 466-468.

[27] Nagao, K., Inoue, N.,Wang, Y.M., and Yanagita, T., 2005, J. Nutr., 135, 9-13.

[28] Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randal, R.J., 1951, J. Biol. Chem., 193, 265-275.

[29] Duncan, D.B., 1955, Biometrics, 11, 1-42.

[30] Hui, T.Y., Olivier, L.M., Kang, S., and Davis, R.A., 2002, J. Lipid Res., 43, 785-793.

[31] Elzinga, B.M., Havinga, R., Baller, J.F.W., Wolters, H., Bloks, V., Mensenkamp, A.R., Kuipers, F., Henkjan, J., and Verkade, H.J., 2002, Biochim. Biophys. Acta, 1573, 9-20.

[32] Eto, N., Miyata, Y., Ohno, H., and Yamashita, T., 2005, Nephrol. Dial. Transplant., 20, 1378-1384.

[33] Yanagita, T., Han, S-Y., Wang, Y-M., Tsuruta, Y., and Anno, T., 2003, Nutrition, 19,140-143.

[34] Buang, Y., Wang, Y.M., Cha, J.Y., Nagao, K., and Yanagita, T., 2005, Nutrition, 21, 867-873.

[35] Buang, Y., Cha, J.Y., Nagao, K., Wang, Y.M., Inoue, N., and Yanagita, T., 2004, J. Nutr. Sci. Vitaminol., 50, 272-276.

DOI: https://doi.org/10.22146/ijc.21469

Article Metrics

Abstract views : 361 | views : 360

Copyright (c) 2010 Indonesian Journal of Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.


Indonesian Journal of Chemistry (ISSN 1411-9420 / 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

Analytics View The Statistics of Indones. J. Chem.