Djoko Agus Purwanto(1*), Retno Pudji Rahayu(2), A. Toto Purnomo(3)

(1) Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Airlangga University
(2) Faculty of Dentistry, Airlangga University
(3) Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Airlangga University
(*) Corresponding Author


Green tea and its polyphenols have been studied extensively as cancer chemopreventive agents in recent years. However, the mechanisms of action are still not clearly understood. Some researchers suggest that immune system plays important role to destroy cancer cells. Because of that reason, the present study was designed to analyse the effects of oral administration standardized green tea water extract on increasing of IFN-g blood concentration and to elucidate possible mechanisms involved in the inhibitory action of the cancer development. Two groups (male and female) of 5 rats have given p.o. administration 1.25% of standardized green tea water extract and got 300 mg of (-)-epigallocatechin gallate (EGCG)/kg body weight, while two groups others (male and female) were used as control. We found that IFN-g blood concentration on male and female Wistar rat are significantly increase with 13.11% and 17.59%, respectively (p<0.05) by ELISA method, whereas control had no increase. These results indicate that standardized green tea water extract has possibility to prevent and suppress cancer development through enhancing IFN-g expression and other immunosurveillance component.


Green tea water extract; IFN-γ; ELISA

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[1] Wu, A.H., Yu, M.C., Tseng, C.C., Hankin, J., and Pike, M.C., 2003, Int. J. Cancer, 106, 4, 574–579.

[2] Fujiki, H., 2005, Chem. Rec., 5, 3, 119–132.

[3] Navindra, P.S., Susanne, M.H., Niu, Y., Rupo, L., Samuel, S.H., and Heber David, H.H., 2006, J. Agric. Food Chem., 54, 1599–1603.

[4] Peters, U., Poole, C., and Arab, L., 2001, Am. J. Epidemiol., 154, 6, 495–503.

[5] Jian, L., Xie, L.P., Lee, A.H., and Binns, C.W, 2004, Int. J. Cancer, 108, 1, 130–135.

[6] Ji, B.T., Chow, W.H., Hsing, A.W., McLaughlin, J.K., Dai, Q., Gao, Y.T., Blot, W.J., and Fraumeni, J.F., Jr., 1997, Int. J. Cancer, 70, 3, 255–258.

[7] Gao, Y.T., McLaughlin, J.K., Blot, W.J., Ji, B.T., Dai, Q., and Fraumeni, J.F., Jr., 1994, J. Natl. Cancer Inst., 86, 11, 855–858.

[8] Yang C.S., and Landau J.M., 2000, J. Nutr., 130, 24092412.

[9] Yang, C.S., Maliakal, P., and Meng, X., 2002, Annu. Rev. Pharmacol. Toxicol., 42, 25–54.

[10] Nagata, C., Kabuto, M., and Shimizu, H., 1998, Nutr. Cancer, 30, 21–24.

[11] Nakachi, K., Suemasu, K., Suga, K., Takeo, T., Imai, K., and Higashi, Y., 1998, Jpn. J. Cancer Res., Bowman, T., Garcia, R., Turkson, J., and Jove, R. 2000, Oncogene, 19, 2474–2488.

[12] Seliger, B., Hammers, S., Höhne, A., Zeidler, R., Knuth, A., Gerharz, C.D., and Huber, C., 1997, Clin. Cancer Res., 3, 573–578.

[13] Reid, G.S.D., Shan, X., Coughlin, C.M., Lassoued, W., Pawel, B.R., Wexler, L.H., Thiele, C.J., Tsokos, M., Pinkus, J.L., Pinkus, G.S., Grupp, S.A., and Vonderheide, R.H., 2009, Clin. Cancer Res., 15, 21, 6602–6608.

[14] Dong, Z., Ma, W., Huang, C., and Yang, C.S., 1997, Cancer Res., 57, 4414–4419.

[15] Seeram, N.P., Henning, S.M., Niu, Y., Lee, R., Scheuller, H.S., and Heber, D., 2006, J. Agric. Food Chem., 54, 5, 1599–1603.

[16] Ochsenbein, A.F., and Zinkernagel. R.M., 2000. Immunol. Today, 21, 624–630.

[17] Miedema, F., Meyaard, L., Koot, M., Klein, M.R., Roos, M.T., Groenink, M., Fouchier, R.A., Van't Wout, A.B., Tersmette, M., and Schellekens, P.T., 1994, Immunol. Rev., 140, 35–72.

[18] Matsunaga, K., Klein, T.W., Friedman, H., and Yamamoto,Y., 2001, Infection and Immunity, 69, 3947–3953.

[19] Bowman, T., Garcia, R., Turkson, J., and Jove, R., 2000, Oncogene, 19, 2474–2488.

[20] Fujiki, H.J., 1999, Cancer Res. Clin. Oncol., 125, 589–597.

[21] Conney, A.H., Lu, Y., Xie, J., and Huang, M., 1999, Proc. Soc. Exp. Biol. Med., 220, 229–233.

[22] Menegazzi, M., Tedeschi, E., Dussin, D., De Prati, A.C, Cavalieri, E., Mariotto, S., and Suzuki, H., 2001, FASEB J., 15, 1309–1311.


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