Differentiation of Curcuma longa, Curcuma xanthorrhiza and Zingiber cassumunar by Thin Layer Chromatography Fingerprint Analysis

https://doi.org/10.22146/ijc.21423

Mohamad Rafi(1*), Eti Rohaeti(2), Ali Miftahudin(3), Latifah K. Darusman(4)

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Kampus IPB Dramaga, Bogor
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Kampus IPB Dramaga, Bogor
(3) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Kampus IPB Dramaga, Bogor
(4) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Kampus IPB Dramaga, Bogor
(*) Corresponding Author

Abstract


Turmeric (Curcuma longa), java turmeric (Curcuma xanthorrhiza) and cassumunar ginger (Zingiber cassumunar) are widely used in traditional Indonesian medicine. These three herbs have relatively similar rhizomes colour so it is difficult to be differentiated especially if they are in powder form. A rapid and reliable method, thin layer chromatography (TLC) fingerprint, has been developed in order to identify, authenticate and differentiate these three herbs through fingerprint profile of chemical compounds. TLC fingerprints of the three herbs were obtained by visualization of separate zones with visible and UV (254 and 366 nm) light. The TLC fingerprint pattern is different each other and showed a specific marker zones respectively. Therefore, TLC fingerprint can be utilized for identification, authentication and differentiation method in quality control of the three herbs tested.

Keywords


Curcuma longa; Curcuma xanthorrhiza; Zingiber cassumunar; TLC fingerprint; quality control

Full Text:

Full Text PDF


References

[1] Soares, P.K., and Scarminio, I.S., 2008, Phytochem. Anal., 19, 1, 78–85.

[2] Zhao, L., Huang, C., Shan, Z., Xiang, B., and Mei, L., 2005, J. Chromatogr. B, 821, 1, 67–74.

[3] Schibli, A., and Reich, E., 2005, J. Planar. Chromatogr. - Mod. TLC, 18, 1, 34–38.

[4] Koll, K., Reich, E., Blatter, A., and Veit, M., 2003, J. AOAC Int., 86, 5, 909–915.

[5] Chen, S.B., Liu, H.P., Tian, R.T., Yang, D.J., Chen, S.L., Xu, H.X., Chan, A.S.C., and Xie, P.S., 2006, J. Chromatogr. A, 1121, 114–119.

[6] Cimpoiu, C., Hosu, A., Briciu, R., Miclaus, V., 2007, J. Planar. Chromatogr. - Mod. TLC, 20, 6, 407–410.

[7] Liu, X.Q., Du, L.L., Li, W.W., Guan, H.F., and Liu, F., 2008, Chem. Pharm. Bull., 56, 175, 75–78.

[8] Ankli, A., Reich, E., and Steiner, M., 2008, J. AOAC Int., 91, 6, 1257–1264.

[9] Widmer, V., Reich, E., and deBatt, A., 2008, J. Planar. Chromatogr. - Mod. TLC, 21, 21–26.

[10] Vermaak, I., Hamman, J.H., and Viljoen, A.M., 2009, S. Afr. J. Bot., 76, 119–124.

[11] Badan Pengawas Obat dan Makanan RI., 2005, Monografi Ekstrak Tumbuhan Obat Indonesia Volume 1, Badan Pengawas Obat dan Makanan RI, Jakarta,

[12] Jiang, Y., David, B., Tu, P., and Barbin, Y., 2010, Anal. Chim. Acta, 657, 1, 9–18.



DOI: https://doi.org/10.22146/ijc.21423

Article Metrics

Abstract views : 4562 | views : 3680


Copyright (c) 2011 Indonesian Journal of Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 


Indonesian Journal of Chemistry (ISSN 1411-9420 /e-ISSN 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

Web
Analytics View The Statistics of Indones. J. Chem.