ENRICHMENT OF α-( ALPHA) LINOLENIC ACID OF BASIL SEED OIL, Ocinum Basillium L. BY FRACTIONAL CRYSTALIZATION AND CRYSTALIZATION IN UREA INCLUSION COMPLEXES

https://doi.org/10.22146/ijc.21415

Warsito Warsito(1*), Jumina Jumina(2), Chairil Anwar(3), Rurini Retnowati(4), Ahmad Ghanaim(5), Suleman Duengo(6)

(1) Department of Chemistry, Brawijaya University, Jl. Veteran Malang 65145
(2) Chemistry Department, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Yogyakarta 55281
(3) Chemistry Department, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Yogyakarta
(4) Department of Chemistry, Brawijaya University, Jl. Veteran Malang 65145
(5) Department of Chemistry, State Islamic University Maulana Malik Ibrahim, Jl. Gajayana 50 Malang 65145
(6) Department of Chemistry, Gorontalo State University, Jl. Jenderal Sudirman No 6, Gorontalo
(*) Corresponding Author

Abstract


Enrichment of α-(alpha) linolenic acid (ALA) of basil seed oil, Ocinum basilicum L. can be done by fractional crystalization and crystalization of fatty acid in urea inclusion complexes (UIC) methods. In this research, the ALA of fatty acid of basil seed oil was fractionated by fractional crystallization in methanol solution at -3, -13 and -25 °C and by crystallization in urea solution (ratio 1:2) at 4, 2, -6 and -8 °C. The ALA percentages were analyzed by GC and GC/MS. The results showed that percentage of ALA obtained from fractional crystallization at -25 °C increase from 65.16 to 91.40, and acquired from UIC is 98.8 at 2 °C

Keywords


ALA; fractional crystallization; urea inclusion complexes; basil seed oil (Ocinum basilicum L.)

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References

[1] Bist, S., Tao, B.Y., and Mohtar, S.A., 2007, Method for Preparation, Use and Separation of Fatty Acid Esters, United State Patent Application Publication, Pub. No: US 2007/0251141 A1, Date of Pub.: Nov. 1, 2007

[2] Gurbuz, B., Cosge, B., and Kiralan, B., 2007, Int. J. Nat. Eng. Sci., 1, 3, 11–15.

[3] Yang, L., Leung, K.Y., Cao, Y., Huang, Y., Ratnayake, W.M.N., and Zhen-Yu, C., 2005, Br. J. Nutr., 93, 433–438.

[4] Nagao, K., and Yanagita, T., 2005, J. Biosci. Bioeng., 100, 2, 152–1578.

[5] Angers, P., Morales, M.R., and Simon, J.R., 1996, J. Am. Oil Chem. Soc., 73, 3, 393–395.

[6] Guil-Gurerrero, J.L., Campra-Madrid, P., and Navarro-Juárez, 2003, Grasas Aceites, 54, 2, 116–121.

[7] Lopéz-Martinéz, J.C., Campra-Madrid, P., and Guil-Guerrero, J.L., 2004, J. Biosci. Bioeng., 97, 5, 294–298.

[8] Hayes, D.G, Van Alstine, J.M., and Setterwall, F., 2000, J. Am. Oil Chem. Soc., 77, 2, 207–213.

[9] Mendes, A., Lopez da Silva, T., and Reis, A., 2007, Food Technol. Biotechnol., 45, 1, 38–44.

[10] Wu, M., Ding, H., Wang, S., and Xu, S., 2008, J. Am. Oil Chem. Soc., 85, 677–684.

[11] Tou, P.G., and Bahru, J., 2009, Quality of Crude Oils and Fats and Recovery of Minor Components, United State Patent, Patent No : US 7,507,847 B2, Date of Patent : Mar. 24, 2009.

[12] Ohlan, R., Narasimhan, B., Ohlan, S., Narang, R., and Judge, V., 2008, Org. Commun., 1, 2, 24–32.

[13] Sajilata, M.G., Singhal, R.S., and Kamat, M.Y., 2008, Food Chem., 109, 3, 580–586.

[14] Ahmed, S.U., Reddy, K.K., Swathy, S.L., Singh, S.K., Kanjilal, S., Prasad, R.B.N., and Pandey, A., 2009, Food Res. Int., 42, 4, 449–453.



DOI: https://doi.org/10.22146/ijc.21415

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