INVESTIGATION ON THE MORPHOLOGY AND PROPERTIES OF AGGREGATE STRUCTURES OF NATURAL PHOSPHOLIPIDS IN AQUEOUS SYSTEM USING CRYO-TEM

https://doi.org/10.22146/ijc.21372

Dwi Hudiyanti(1*), Tri Joko Raharjo(2), Narsito Narsito(3), Sri Noegrohati(4)

(1) Department of Chemistry, Diponegoro University, Jl. Prof. H. Sudharto, S.H., Tembalang, Semarang, 50275
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281
(3) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281
(4) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, 55281
(*) Corresponding Author

Abstract


Cryogenic transmission electron microscopy (Cryo-TEM) was used to investigate the aggregates morphology and properties of candle tree (Aleurites moluccana) endosperm, sesame (Sesamum indicum L. syn.) seeds, and coconut (Cocos nucifera) endosperm phospholipids in dilute aqueous system. The micrographs showed that candle tree phospholipids formed planar bilayer and cluster of vesicles with lipid droplets, while coconut and sesame phospholipids formed well-defined unilamellar vesicles. The vesicles size could be as small as 50 nm in diameter. Coconut phospholipids also showed a good bending ability. Formation of clusters of vesicles was also found in coconut phospholipids dispersion, but this cluster was easily broken by extrusion through a small pore membrane.

Keywords


vesicle; liposome; Cryo-TEM; phospholipids; coconut; sesame seed; candle tree

Full Text:

Full Text PDF


References

[1] Gregor, C., 1993, Phospholipids Handbook, CRC Press, New York, 29–31.

[2] Leray, C., 2011, Phosphoglycerides, http://www.cyberlipid.org/phlip/pgly02.htm, accessed on November 11, 2011.

[3] Johnsson, M., and Edwards, K., 2003, Biophys. J., 85, 3839–3847.

[4] Bergstrand, M., and Edwards, K., 2001, Langmuir, 17, 11, 3245–3253.

[5] Fricker, G., Kromp, T., Wendel, A., Blume, A., Zirkel, J., Rebmann, H., Setzer, C., Quinkert, R., Martin, F., and Müller-Goymann, C., 2010, Pharm. Res., 27, 8, 1469–1486.

[6] Castor, T.P., 2005, Curr. Drug Deliv., 2, 4, 329–340.

[7] Nohynek, G.J., Lademann, J., Ribaud, C., and Roberts, M.S., 2007, Crit. Rev. Toxicol., 37, 251–277.

[8] Patino, J.M.R., Caro, A.L., Nino, M.R.R., Mackie, A.R., Gunning, A.P., and Morris, V.J., 2007, Food Chem., 102, 2, 532–541.

[9] Hudiyanti, D., 2007, Aggregasi Fosfolipida dalam Medium Akuos, Proceeding of Seminar Nasional Kimia dan Pendidikan Kimia, Department of Chemistry, Diponegoro University, Semarang, 85–90.

[10] Almgren, M., Edwards, K., and Karlsson, G., 2000, Colloids Surf., A, 174, 3–21.

[11] Hudiyanti, D., Raharjo, T.J., Narsito, and Noegrohati, S., 2008, Isolasi dan Karakterisasi Fosfolipida Nabati, Proceeding of Seminar Nasional Kimia dan Pendidikan Kimia, Department of Chemistry, Sebelas Maret University, Surakarta, 222–226.

[12] Weesman, P., Stromstedt, A.A., Malmsten, M., and Edwards, K., 2008, Biophys. J., 95, 9, 4324–4336.

[13] Ferreira, D.A., Bentley M.V.L.B., Karlsson, G., and Edwards, K., 2006, Int. J. Pharm., 310, 203–212.

[14] Israelachvili, J.N., 1992, Intermolecular and Surface Forces, Academic Press, London, 380–382.



DOI: https://doi.org/10.22146/ijc.21372

Article Metrics

Abstract views : 1728 | views : 1574


Copyright (c) 2012 Indonesian Journal of Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 

Indonesian Journal of Chemistry (ISSN 1411-9420 /e-ISSN 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

Web Analytics View The Statistics of Indones. J. Chem.