Effect of Non Ionic Surfactant Addition to Cellulase Performance in High-Substrate-Loading-Hydrolysis of Palm Oil EFB and Water-Hyacinth

Teuku Beuna Bardant; Sudiyarmanto Sudiyarmanto; Haznan Abimanyu; Aisha Kania Hanum

doi:10.22146/ijc.21326

Effect of Non Ionic Surfactant Addition to Cellulase Performance in High-Substrate-Loading-Hydrolysis of Palm Oil EFB and Water-Hyacinth

https://doi.org/10.22146/ijc.21326

Teuku Beuna Bardant^(1*), Sudiyarmanto Sudiyarmanto⁽²⁾, Haznan Abimanyu⁽³⁾, Aisha Kania Hanum⁽⁴⁾

(1) Research Center for Chemistry, Indonesian Institute of Science, Komplek PUSPIPTEK Serpong Tangerang Banten 15413
(2) Research Center for Chemistry, Indonesian Institute of Science, Komplek PUSPIPTEK Serpong Tangerang Banten 15413
(3) Research Center for Chemistry, Indonesian Institute of Science, Komplek PUSPIPTEK Serpong Tangerang Banten 15413
(4) Department of Chemistry, Faculty of Science, University of Diponegoro, Jl. Prof. Soedharto Kampus Tembalang Semarang
(*) Corresponding Author

Abstract

Enzymatic hydrolysis with high substrate loading of palm oil (Elaeis guineensis) empty fruit bunch (EFB) and water-hyacinth (Eichhornia crassipes) were investigated as a prior part of ethanol production from lignocelluloses. Commercial surfactant Span 85 and Tween 20 were used as cellulase performance enhancer in hydrolysis process with substrate loading above 20% (^w/_w). Cellulase performances were compared based on hydrolysis conversion. Hydrolysis conversions of EFB using cellulase with concentration 10 and 15 FPU/g-substrate was 38.55% and 88.80% respectively. Addition 2% (^v/_v) of Tween 20 to EFB hydrolysis reaction with cellulase concentration 10 FPU/g-substrate gave the conversion 87.30%. This addition enhance the cellulase performance up to 226.5% or similar with the performance of cellulase 15 FPU/g substrate. Addition 2% (^v/_v) of Span 85 to the similar reaction only enhances cellulase performance to 174.7%. Hydrolysis conversion of boiling-pretreated water-hyacinth and autoclave-pretreated water-hyacinth using cellulase 15 FPU/g-substrate was 45.84% and 52.29% respectively. Addition 2% (^v/_v) of Tween 20 and Span 85 to boiling-pretreated water-hyacinth hydrolysis with cellulase concentration 15 FPU/g-substrate enhance cellulase performance of 128.9% and 153.5% respectively. Addition
1% (^v/_v) of Tween 20 and Span 85 to the similar reaction with cellulase concentration 10 FPU/g-substrate gave conversions 51.00% and 53.79% respectively, or similar with conversion of autoclave-pretreated water-hyacinth hydrolysis with 15 FPU/g-substrate.

Keywords

cellulose enzymatic hydrolysis; Tween 20; Span 85; bioethanol lignocellulose

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DOI: https://doi.org/10.22146/ijc.21326