The Fermentation of Green Algae (Spirogyra majuscule Kuetz) using Immobilitation Technique of Ca-Alginate for Saccharomyces cerevisiae Entrapment

https://doi.org/10.22146/ijc.21319

Atmanto Heru Wibowo(1*), Lailatul Mubarokah(2), Adhitasari Suratman(3)

(1) Central Chemistry Laboratory, University of Sebelas Maret, Jl. Ir. Sutami No. 36 A, Surakarta 57126
(2) Department of Chemistry, University of Sebelas Maret, Jl. Ir. Sutami No. 36 A, Surakarta 57126
(3) Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281
(*) Corresponding Author

Abstract


A study of batch fermentation of green algae (Spirogyra majuscula Kuetz) from Pengging Lake, Boyolali, Central Java for bioethanol source using immobilization technique of Ca-alginate for Saccaromyces cerevisiae entrapment has been done. The scope of the study emphasized on the best condition for the processes of hydrolysis and fermentation. Concentration of sulfuric acid and hydrolysis time were varied with 0.1, 0.2, 0.3, 0.4 and 0.5 M for 30, 90, 150, 210, 270, 330, 360, 390, 420, and 450 min to obtain the maximum glucose content of UV analysis. Na-alginate : yeast ratio and fermentation time were varied with 1:5, 2:4, 3:3, 4:2 and 5:1 (w/w) for 1, 2, 3, 4, 5, 6 and 7 days. Distillation at 70-80 °C was deployed to purify the fermentation product. The ethanol content in the product was analyzed using gas chromatography-flame ionization detector (GC-FID). The result of study showed that maximum glucose content was obtained 2.1% from 0.2 M sulfuric acid for 6 h of hydrolysis. Maximum ethanol content was obtained 54.1% from 2:4 ratio of Na-alginate : yeast (w/w) for 4 days of fermentation. The study also concludes that immobilization technique of Ca-alginate increase alcohol content compared to without immobilization of green-algae fermentation.

Keywords


Ca-alginate; immobilization; green algae; bioethanol

Full Text:

Full Text PDF



DOI: https://doi.org/10.22146/ijc.21319

Article Metrics

Abstract views : 664 | views : 1710


Copyright (c) 2013 Indonesian Journal of Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 


Indonesian Journal of Chemisty (ISSN 1411-9420 / 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

Web
Analytics View The Statistics of Indones. J. Chem.