Salahuddin Salahuddin(1*), Muhammad Hanafi(2), Hariyanti Hariyanti(3)

(1) Faculty of Pharmacy, University of Uhamka, Perumnas Klender 13460, Jakarta
(2) Research Centre for Chemistry – LIPI, Kawasan Puspiptek, Tangerang 15314
(3) Faculty of Pharmacy, University of Pancasila, Lenteng Agung, Jakarta 12640
(*) Corresponding Author


The novel compound of 2-hydroxy-N-phenylnicotinamide was synthesized from modification of the UK-3A compound, which was known biologically active to inhibit bacterial and cancer cells growth. Synthesis of this compound was carried out in one-step reaction. Analog UK-3A compound namely 2-hydroxy-N-phenylnicotinamide was synthesized from 2-hydroxynicotinic acid as the starting material using amidation reaction with aniline in presence of DCC/DMAP in pyridine. Analog UK-3A was analyzed and identified using TLC, FT-IR, LC-MS and FT-NMR spectrofotometer. The yield after purification was 42.97%. The IC50 value 85 μg/mL was obtained from cytotoxicity test against P388 Murine Leukemia cells. It indicated that the synthesis products were sufficiently potential for leukemia P388 treatment.


2-hydroxy-N-phenylnicotinamide; UK-3A; Amidation; P388 Murine Leukemia cells

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